Or_Gro’s Tissue Culture Journal

led1k

led1k

Well-Known Member
Or_Gro said:
Simple, sterile glove box:

Click to expand...
This popped up as upper left choice after watching the video. Plastic collars rather than cardboard tubes and weather stripping for the win? I think I would use the Henry's 900 rather than plastic epoxy.
 
Or_Gro

Or_Gro

Well-Known Member
led1k said:
This popped up as upper left choice after watching the video. Plastic collars rather than cardboard tubes and weather stripping for the win? I think I would use the Henry's 900 rather than plastic epoxy.
Click to expand...
A good one! Lots out there, for diff uses; this was well-thought out and well-made.

Couple thoughts, now that i’ve seen a bunch:

1. Take the Keep It Simple, Stupid approach, just the necessary modification, bells and whistles are a waste of time - you’re working in a tote, for godsakes!

2. Biggest pvc diameter you can get for ports - to get hands thru

3. Narrowest length pvc ports (on sideview) - for range of motion; you don’t need 2 pvc fittings as above. If you need 2 pieces, get a screwable flange and base...

4. For a stronger glove-port connection, attach gloves to pvc port using a worm-drive hose clamp, seal with duct tape.

5. Use gloves that are way bigger than your hands...you will be reminded of this, if you forget...when trying to pull your hands out of them.

6. Consciously decide whether you want tote lid to be the base or the top of your glovebox.

7. Learn how to sanitize glovebox and how to use sterile technique....or you will be pissed at all the time and money you wasted.

https://biocyclopedia.com/index/biotechnology_methods/tissue_culture_techniques/sterile_methods_in_plant_tissue_culture.php
 
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led1k

led1k

Well-Known Member
Or_Gro said:
A good one! Lots out there, for diff uses; this was well-thought out and well-made.

Couple thoughts, now that i’ve seen a bunch:

1. Take the Keep It Simple, Stupid approach, just the necessary modification, bells and whistles are a waste of time - you’re working in a tote, for godsakes!

2. Biggest pvc diameter you can get for ports - to get hands thru

3. Narrowest length pvc ports (on sideview) - for range of motion; you don’t need 2 pvc fittings as above. If you need 2 pieces, get a screwable flange and base...

4. For a stronger glove-port connection, attach gloves to pvc port using a worm-drive hose clamp, seal with duct tape.

5. Use gloves that are way bigger than your hands...you will be reminded of this, if you forget...when trying to pull your hands out of them.

6. Consciously decide whether you want tote lid to be the base or the top of your glovebox.

7. Learn how to sanitize glovebox and how to use sterile technique....or you will be pissed at all the time and money you wasted.

https://biocyclopedia.com/index/biotechnology_methods/tissue_culture_techniques/sterile_methods_in_plant_tissue_culture.php
Click to expand...
Excellent points. Another take with 500k views. Not sure about the fold it for storage part but using a vacuum cleaner HEPA bag for intake and vacuum for suction seems like a great setup. I'm guessing sealing/closing the suction side before turning off the vaccum would help.
 
Or_Gro

Or_Gro

Well-Known Member
led1k said:
Excellent points. Another take with 500k views. Not sure about the fold it for storage part but using a vacuum cleaner HEPA bag for intake and vacuum for suction seems like a great setup. I'm guessing sealing/closing the suction side before turning off the vaccum would help.
Click to expand...
There’s another one where the guy has gas connections, for filling with argon....
 
Or_Gro

Or_Gro

Well-Known Member
led1k said:
F that! What did you get? Mine (https://www.horticulturesource.com/tissueponics-starter-kit-36-p27106)
arrived yesterday and I only broke one test tube unpacking. 35 tubes and counting...
Click to expand...
I already have the one you received...and i plan to start it early next week w cannabis and some other cuttings, as soon as my clone tent is freed up. This is basically an in vitro cloning kit, that takes 8-11 weeks to get a transplantable rooted cutting. I do same thing in my diy aerocloner, in 10-14 days.

The real benefit of tissue culture of canabis is the multiplication/replication stage, where you can exponentially expand the number of clones.

This kit is the one i linked above, where they f’d up the order.

AC10C65A-5820-4782-966D-FFC0214ADE71.jpeg

It is the kit that enables you to go from cutting to fully rooted miniature plant in vitro, like this:

5BBB97E8-649C-4752-8E95-3E670479E078.jpeg

I’ll start this one in a few weeks.
 
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led1k

led1k

Well-Known Member
Or_Gro said:
I already have the one you received...and i plan to start it early next week w cannabis and some other cuttings, as soon as my clone tent is freed up. This is basically an in vitro cloning kit, that takes 8-11 weeks to get a transplantable rooted cutting. I do same thing in my diy aerocloner, in 10-14 days.

The real benefit of tissue culture of canabis is the multiplication/replication stage, where you can exponentially expand the number of clones.

This kit is the one i linked above, where they f’d up the order.

View attachment 4346838

It is the kit that enables you to go from cutting to fully rooted miniature plant in vitro, like this:

View attachment 4346836

I’ll start this one in a few weeks.
Click to expand...
I'm with you on the aerocloner! Just ran a batch and went 14/14 with 12-17 days to transplant. Dredge the roots in Mykos and into solos.

Maybe I jumbled up my memory but I thought the tissueponics kit allows to double an explant in size in 2 weeks and then straight into rapid rooters. Reading that back it now sounds far fetched to double but maybe it's the sucrose? I'm hoping to make a clone of a plant already well into flower. What do you think? Chance in hell?
 
led1k

led1k

Well-Known Member
No hepa filter or vacuum here. KISS for sure. "Super super clean way, a couple sprays with alcohol..."
 
Or_Gro

Or_Gro

Well-Known Member
led1k said:
I'm with you on the aerocloner! Just ran a batch and went 14/14 with 12-17 days to transplant. Dredge the roots in Mykos and into solos.

Maybe I jumbled up my memory but I thought the tissueponics kit allows to double an explant in size in 2 weeks and then straight into rapid rooters. Reading that back it now sounds far fetched to double but maybe it's the sucrose? I'm hoping to make a clone of a plant already well into flower. What do you think? Chance in hell?
Click to expand...
I’d try it...it’s the way to learn....take something near bottom.....
 
Or_Gro

Or_Gro

Well-Known Member
led1k said:
No hepa filter or vacuum here. KISS for sure. "Super super clean way, a couple sprays with alcohol..."
Click to expand...
Do it in a small, draft-free space...i’m gonna use an empty tent to put them in the tubes...

Notice on instructions, he doesn't say anything about how/when to put nutrient into tubes....make sure you review on vids before starting....
 
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