STRAINLY

Rurumo

Well-Known Member
I have but it wasn't with root testing it was the petioles. That shit aint SFV OG tho its GG4
Same. I used Genie labs, and used petiol from months old leaf, 3 of AJN's strains tested Neg (this was pre-Gushers, when the positives started rolling in). One plant tested positive out of 4 strains tested at that time (Cereal Milk-not from AJN), so I'm pretty confident in the lab, and protocols. Genie takes root material now too, not a bad option if you don't need 10 tests at a time, and the price has gone down a bit too.
 

JustBlazin

Well-Known Member
Runtz and Marshmello OG I have. Even before the root test recommendation using 3-5 samples from the petioles was pretty accurate, up 90’s%..
Stem and petioles test are only 30-75% accurate as per tumi study. I would never base my plants being negative using those percentage. That's exactly how this viroid got so rampant passing positive plants that test negative with stem and petioles tests.
I would only trust root testing to say a plant is negative.
My whole garden was wiped out do to these stupid stem and petioles tests saying positive plants were negative.
 

nxsov180db

Well-Known Member
Stem and petioles test are only 30-75% accurate as per tumi study. I would never base my plants being negative using those percentage. That's exactly how this viroid got so rampant passing positive plants that test negative with stem and petioles tests.
I would only trust root testing to say a plant is negative.
My whole garden was wiped out do to these stupid stem and petioles tests saying positive plants were negative.
That's on the new growth that's only 30 something percent. I wouldn't ever and never have taken new growth petioles. The percentages of accuracy listed is for 1 sample, when you're taking 3-5 samples it would be pretty unlikely that you would get a false negative but sure it could happen... The odds of a 70% outcome not coming in 5 times in a row is 1.875%. Root testing being 100% only makes sense of course, and I like being able to test multiple moms in 1 test.
 

JustBlazin

Well-Known Member
That's on the new growth that's only 30 something percent. I wouldn't ever and never have taken new growth petioles. The percentages of accuracy listed is for 1 sample, when you're taking 3-5 samples it would be pretty unlikely that you would get a false negative but sure it could happen... The odds of a 70% outcome not coming in 5 times in a row is 1.875%. Root testing being 100% only makes sense of course, and I like being able to test multiple moms in 1 test.
If it was that easy to get positive results from testing like that I doubt the industry would be plagued by this and dark heart would probably still be in business, if you dont test the exact right branch your not going to get a positive result. But that's just my opinion
 

nxsov180db

Well-Known Member
If it was that easy to get positive results from testing like that I doubt the industry would be plagued by this and dark heart would probably still be in business, if you dont test the exact right branch your not going to get a positive result. But that's just my opinion
Numbers and math don't lie. The outbreak is because no one is testing really, how many mega nurseries with 200 mother plants are testing? Dark heart doesn't really test what you send them unless you're an actual company so that's a problem, look at AJN.. Then you've got the DIY tests some people are using that can only detect high viroid levels.. When you mention the need to test the exact branch that would only hold true if the plant had very recently been infected. Any clone you buy that you veg into a teen or mom should pretty much have a viroid load of some level in any part of it. I've spent a few thousand in testing with Tumi over the last year and a half, I had a ton of positives at first, I've yet to have a mom that tested negative now test positive since switching to the root test. But I also used 5 samples per vial when it was the petiole. I have a clone I bought a few months ago (before the root tests) from pig tail gardens, let it veg for a week or so, took 3-4 petiole samples, and it came back as a low viroid level. I think I've had 3 so far that have came back as a low viroid level. Tumi suggested on all 3 occasions to retest in a few weeks and asked about possible cross contamination with the samples but I just tossed whatever failed. Those 3 times are about as close to a false negative as I've had.
 

waterproof808

Well-Known Member
Is Dark heart really out of business?

Side note: I saw first class genetics picked up a bunch of cuts from the emerald cup and a bunch of them tested positive for HLVd
 

JustBlazin

Well-Known Member
I was talking about the site you used to be able to see what clones they had that's not up anymore darkhearnursery.com that is linked on there twitter account. I've seen that site you linked and am not sure what to think about it. Can't really do anything on it but contact them?
Then there's the fact there's no mention of root testing or any valuable info on the viroid plus it doesn't seem like there's been any updates since last winter. The twitter account hasn't been updated since July along with the Instagram account before it got deleted.
Are they still doing clone drops?
Would think they would announce them somewhere if they were?
 

jcdws602

Well-Known Member
I was talking about the site you used to be able to see what clones they had that's not up anymore darkhearnursery.com that is linked on there twitter account. I've seen that site you linked and am not sure what to think about it. Can't really do anything on it but contact them?
Then there's the fact there's no mention of root testing or any valuable info on the viroid plus it doesn't seem like there's been any updates since last winter. The twitter account hasn't been updated since July along with the Instagram account before it got deleted.
Are they still doing clone drops?
Would think they would announce them somewhere if they were?
I honestly don’t know. I was just linking the website I knew worked but yes I concur that the website is just a lot information without any direct information on how to purchase any of their services/products. I personally don’t have any interest in what they are offering. But who knows I can only speculate with the limited amount of information I’m working with.
 

Rurumo

Well-Known Member
Numbers and math don't lie. The outbreak is because no one is testing really, how many mega nurseries with 200 mother plants are testing? Dark heart doesn't really test what you send them unless you're an actual company so that's a problem, look at AJN.. Then you've got the DIY tests some people are using that can only detect high viroid levels.. When you mention the need to test the exact branch that would only hold true if the plant had very recently been infected. Any clone you buy that you veg into a teen or mom should pretty much have a viroid load of some level in any part of it. I've spent a few thousand in testing with Tumi over the last year and a half, I had a ton of positives at first, I've yet to have a mom that tested negative now test positive since switching to the root test. But I also used 5 samples per vial when it was the petiole. I have a clone I bought a few months ago (before the root tests) from pig tail gardens, let it veg for a week or so, took 3-4 petiole samples, and it came back as a low viroid level. I think I've had 3 so far that have came back as a low viroid level. Tumi suggested on all 3 occasions to retest in a few weeks and asked about possible cross contamination with the samples but I just tossed whatever failed. Those 3 times are about as close to a false negative as I've had.
You make some good points. I totally believe that (besides the profit motive) testing companies tell people to test 3+ times just because they know most people are just sending in fresh samples (low viroid levels) from Strainly cuts and the first test will be a false negative for a lot of those (except maybe for roots now? idk). I always like to grow out a cut for a month in isolation before testing it, but that's just an arbitrary # and might not make a difference, it just makes sense to me.
 

F_T_P!

Well-Known Member
You make some good points. I totally believe that (besides the profit motive) testing companies tell people to test 3+ times just because they know most people are just sending in fresh samples (low viroid levels) from Strainly cuts and the first test will be a false negative for a lot of those (except maybe for roots now? idk). I always like to grow out a cut for a month in isolation before testing it, but that's just an arbitrary # and might not make a difference, it just makes sense to me.
Sometimes the AIDS doesnt show up til you flip or of it gets stressed out. I say run it all the way giving it different stresses along the way to see if it triggers. Only way to be sure imo.
 
quel fiore automatico è stato appena elencato nelle ultime 24 ore …
Il tuo buffo fratello...queste sono le normali righe S1 View attachment 5218140
[/CITAZIONE]
Ho anche comprato semi dal deposito di semi sociali.
10 semi RS11 e 5 semi Dolato.
5 Dolato fioriscono da 3 settimane.
Ho buttato via 5 semi di RS11. 1 seme è entrato in fioritura con luce a 18/6. Altri 4 semi sono rimasti molto piccoli in veg. Ho mostrato la foto a un amico che ha acquistato RS11 da GSRH. Mi ha detto che non è genuina.
Ho visto foto di dolato in giro. E sono totalmente diversi dalle foglie.
Scusa per il mio cattivo inglese.View attachment 20221223_131922.jpg
 
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Ogexoticsonly

Active Member
About two weeks ago I tested with dark heart. I still have their email so I didn’t check the website, but they responded that day. Had the test results three days after they received the samples. In their email packet they send for submission they say you can send root samples.
 

slacker140

Well-Known Member
Weedmaps used to be full of dark heart clones in the Los Angeles area and now I don't see any listed. So it appears they quit doing drops.
 
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