natureboygrower
Well-Known Member
I think taking a couple days off from microdosing is a good idea. Might help reset things a bit so tolerance doesn't increase too dramatically.
Yeah. I don’t think you’re supposed to do it continuously.I think taking a couple days off from microdosing is a good idea. Might help reset things a bit so tolerance doesn't increase too dramatically.
Yeah, suppress inoculations are pretty hit or missMan. I wish you were dealing with a liquid culture. If you think about it, allot of things need to happen just right if you go squirting some spores in a bag.
I think the smartest thing you can do is squirt what you’re gonna squirt into one spot. Agitate the syringe up as much as you can first.
What is your go to method when you are dealing with spores just curious.Yeah, suppress inoculations are pretty hit or miss
Agar dishes and then sector selection then another dish then liquid culture then test.What is your go to method when you are dealing with spores just curious.
Agar dishes and then sector selection then another dish then liquid culture then test.
Nice.Agar dishes and then sector selection then another dish then liquid culture then test.
Yes, one drop is plentyNice.
Question. From a swab/spore print, that’s what i do. From a spore syringe you do the same?
Just seems like in a 10cc spore syringe there really isn’t much spores to squirt out on an agar plate. One drop?
You can't do sector selection if you swab the dish. Spores need to find complementary spores and make clamp connections. The closer the spores are together the quicker they select their mates and begin mycelium migration across the plate. So you place your drop dead center of the dish.You could also squirt some of the solution out into a clean shot glass, and use an inoculation loop to stir around in it, and then streak it across the agar surface in a small Z pattern. Or use the T or quadrant methods, etc, which further Isolates any possible contams by spreading it around into multiple different sectors, getting more and more thinned out for each one as you go.
I just watched it again, it's not quite what I described but what is to keep one from simply attaching a needle to the end of the tube and just using that as your inoculation syringe rather than filling individual syringes? Replace the needle after so many innculations.
Kinda like this setup?
Essentially true but it still depends. If I have contamination I "race" the mycelium. This involves germinating a few spores and then placing another "slab" of agar on top sandwitching the grow between the agar. This limits the growth to two dimensions. If the contamination is bacterial (which it most often is) the bacteria will not travel but the mycelium will grow away from the infected spot.I thought the purpose of streaking the patterns with a loop was to spread as many possible contaminants away from the spores over the surface as possible. The thin loop picks up just the right amount of spores you need, without the extra water if you just dip it in. You can also smear out a drop the same way too.
All MSS should be considered non sterile as is right.. so why not go ahead and streak your first plate every time that way? I suppose it would be the best method if you knew your spore solution or print was in fact contaminated, and trying to salvage anything you can.. In the above gif, you would only transfer from sector 5 towards the middle, which would be the most thinned out. More likely to find something clean to take from in that area right? Then, transfer it to the middle of a new plate as usual...
'Stamets Stack' works for me.Yeah. I don’t think you’re supposed to do it continuously.
I did it for a month, my buddy makes them from my strains, i did feel refreshed but i also take enough to trip as often as i can.
But i’d treat micro dosing like shrooming. You take a gram one day. It’s gonna take 2 grams the next day. So maybe 30 days on 30 days off? Then 30 on?
Or just trip balls once a week. That’s about what i do lol.
I imagine that you could hear even moist pieces. You could inject 20 cc or so of sterile waterAnd here's the new AIO bag I just got in the mail today. So dry that if you give it a little shake you can actually hear the pieces rattling around. I'm far from an experienced eye, but doesn't look to be anywhere near field cap to me. Just can't fucking win with these things lately.
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