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  1. coconutbeach

    Plant Tissue Culture Question

    Thanks eDude. We are always trying to keep it simple and successful. You may be interested in one of our simplified methods set up after so much travel and TC presentations...liquid tissue culture starts. Here is a short write-up and pics. Good luck.
  2. coconutbeach

    Tissue culture and cannabis?

    Hi Native. It would be my pleasure. The instructions are the same and we will be producing a cannabis tissue culture kit as soon as we get the legal "go". Here are some TC cannabis pics of the kits in action and a tip sheet for making starts in liquid medium. The media in the Microclone kit...
  3. coconutbeach

    Tissue culture and cannabis?

    Your wish is granted.
  4. coconutbeach

    once again...cloning from fan leaf

    In tissue culture, plants can be created from leaves but it is best when those leaves have been produced themselves in tissue culture. the auxins in the medium seem to create callus tissue and roots in the ordinary course of TC cloning. Sterilizing leaves to put in tissue culture most often...
  5. coconutbeach

    what do I need for tissue culture

    How has that worked out? The kit was assembled for $250 as a bargain to going to the trouble of building one's own kit. Everything in the kit is vital and users are encouraged to build onto it and source new needed items, particularly vessels like baby food jars. Everybody on this list should...
  6. coconutbeach

    Is It Possible To Clone Autos With Tissue Culture?

    It is being tested at this moment using Iranian Autoflower varieties. Will let you know how it works out. Seed starts will be introduced into culture and the donor plants and cultures grown under 18 hours light. Will the tissue culture plants flower in culture even though they are so tiny...
  7. coconutbeach

    Tissue culture and cannabis?

    The first Microclone grow racks held multiple vessels of over 30 varieties on one 2x4 ft shelf under two 54-watt t5's. Regular clone ROOTING is much faster because of the resources put into creating the 6" + cuttings. We still always have some rooted clones to back up the cultures and prove...
  8. coconutbeach

    Tissue culture and cannabis?

    Check out Microclone Cannabis Tissue Culture. Search Google.
  9. coconutbeach

    Cloning a clone of a clone?

    The protocol for cannabis has been used effectively and sold commercially for over ten years now. Wang et al published the first papers because they could do the research in China. Microclone tissue culture kits are sold in hydro stores and online at planttc.com to medically and recreationally...
  10. coconutbeach

    Tissue culture and cannabis?

    What a great space. I am in CO as much as I am at home these days. Your space can make all the clones you need and you will likely still have space left. You will need a filtered air area for growing and handling, kitchen for media prep and dishes, and rooting trays and racks. OUr biggest...
  11. coconutbeach

    Tissue culture and cannabis?

    A typical wash is 10% bleach for 15-20 minutes, however, we much prefer 500ppm of dichlor because the Cl evaporates and typically does need the additional sterile water soak. Dichlor is included in the Microclone TC kits.
  12. coconutbeach

    Tissue culture and cannabis?

    Check out Microclone tissue culture kits at planttc.com or at Alaska Jacks in Palmer.
  13. coconutbeach

    Tissue culture and cannabis?

    Startup cost of Microclone tissue culture. Kit, pressure cooker, tub, rack lights. Less than $400 for three months growth and first 150 rooted clones.
  14. coconutbeach

    Tissue culture and cannabis?

    Four and six ounce polypropylene and glass jars are best for gel culture. We are also using 23mm plastic tubes for introductions and starts. All are included in the Microclone tissue culture kits. planttc.com Make it easy and use 1-1/2" clean vegetative branch tips.
  15. coconutbeach

    tissue culture kit

    Check out our videos for Microclone tissue culture. We do coffee for fun in our workshops from cuttings and from seed. We created the kits in 2005 when we realized we could add food grade preservatives that make tissue culture very successful at home. I have been growing tc plants in my...
  16. coconutbeach

    Deep Tissue Culture

    Clean, vigorous, vegetative branch tips are best for starts. About 1-1/2" tall.
  17. coconutbeach

    Deep Tissue Culture

    That was our kit, the Microclone tissue culture kit. I happened to pick up the mag in Hollywood, CA and saw the article, then realized the photos were my hands! Google "Microclone" for videos and see cannabis tissue culture on Facebook. I wanted to correct the assumption everything had to be...
  18. coconutbeach

    Looking for small carbon Filter

    Is your Walmart filter still working? A 4" Phresh filter can be found at Far North or Alaska Jacks for $100 and last for years and is only about 8 inches long.
  19. coconutbeach

    Plant Tissue Culture

    Branching plant in tissue culture. Each jar yields 5 to 8 new clones per month. From Super Starts kit purchased online. Our best results were on TDZ formula. May have to ask specifically. Included in our kit.
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