Dead Spores

canndo

canndo

Well-Known Member
Through a series of stupid mistakes on my part, I isolated a particular strain I don't want. Fine. I went back to my collection of syringes of various strains and did some dish work with them - only, nothing germinates - NOTHING.

They have been kept in the crisper of my fridge for about 6 months and nothing in them is viable any more. I don't think the crisper has ever been the subject of freezing so what do you folks think happened?
 
T

tibberous

Well-Known Member
I had the exact same thing happen, no idea why.

Did you have them as syringes or where you going spores > agar? I think it might be that the spores need more moisture than the agar provides (just to get going)
 
canndo

canndo

Well-Known Member
syringes - so they were wet, which might be the problem. I've revived 10 year old spores from a dry state on agar.
 
dwcannan

dwcannan

Active Member
you guys know how to use a spore print if so could you tell me how man im just leanrin this shit and ordered the print intead of suringe ive done my research of the prorecces of growing them i just cant find nothin on using a print only suringes?? and any idea how im supposed to germinate the spores in with the rye grains in the jar??
 
dwcannan

dwcannan

Active Member
so basically i would be better off just ordering the suringe with the spores already in it im getting the stuff from spores101 online know anything about them??
 
Bwpz

Bwpz

Well-Known Member
dwcannan said:
so basically i would be better off just ordering the suringe with the spores already in it im getting the stuff from spores101 online know anything about them??
Click to expand...
You can use Spore101, or SporeWorks, or even Micro-Supply.

If you're a beginner just order the syringe.
 
dwcannan

dwcannan

Active Member
oh ok so i can use the print i see well in that case where can i get a suringe at any ideas??
 
Bwpz

Bwpz

Well-Known Member
dwcannan said:
oh ok so i can use the print i see well in that case where can i get a suringe at any ideas??
Click to expand...
You can get them on Spores101 for 9.99+ shipping.

Like I said, if you're a beginner just stick with the needles for the first grow, and then make your own prints.
 
dwcannan

dwcannan

Active Member
well i know they have the suringe with the spores already in them but i ordered the print on accident not paying attention so i would rather just get an empty suringe and use the print instead of buy a full suringe but where the hell do i get one at?
 
Bwpz

Bwpz

Well-Known Member
dwcannan said:
well i know they have the suringe with the spores already in them but i ordered the print on accident not paying attention so i would rather just get an empty suringe and use the print instead of buy a full suringe but where the hell do i get one at?
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It's a pack of 10 for 9.99+shipping.

Also, since you're already going to have needles, look into making a LC (Liquid Culture) so that you can fill up needles and put them directly into your jars. Here's a link:

http://www.shroomtalk.com/forum/index.php?/topic/33975-hippy-piss/
 
dwcannan

dwcannan

Active Member
thats what i need to put into the spores in the rye right the make them germinate before i actually start growin them
 
Bwpz

Bwpz

Well-Known Member
You put the spores from the print into the needle. Then, use the needle to inject your rye jars and a few LC jars. From now on, you can just use your needle to get liquid from the LC and inject that into the rye jars, instead of buying needle or using prints.

Look up each step above on shroomery or shroomtalk, it will show you how to do each if you don't know.
 
dwcannan

dwcannan

Active Member
sweet thanks guys i really appreciate it like i said this is a new experiment for me you can only learn through trial and error im buyin a kit from spores101 so im thinkin that should make all this a little easier instead of gettin all the supplies seperatly so with the liquid culture the spores are in there and i can just fill a suringe and inject it into the rye jars and be good to go
 
Bwpz

Bwpz

Well-Known Member
Here is a finished LC, a lil bit after you inject it with spores:



You shake that up and break it down a lil (some people put a piece of glass or a marble in there to aid in this) and you can pull it into your needle and then inject it into your spawn jar.
 
canndo

canndo

Well-Known Member
get yourself some petri dishes and some agar and make your own cultures - it is very very easy to do with prints and you will have an unlimited supply of culture.
 
Bwpz

Bwpz

Well-Known Member
canndo said:
get yourself some petri dishes and some agar and make your own cultures - it is very very easy to do with prints and you will have an unlimited supply of culture.
Click to expand...
You got a beginner-friendly agar tutorial? I'd like to learn something new tonight anyways, I haven't accomplished shit xD
 
dwcannan

dwcannan

Active Member
ok cool man this is so helpful would i be better off gettin the agar since i already ordered a spore print on accident?? how long does it take to germinate in the LC
 
canndo

canndo

Well-Known Member
Get some agar and a few sleeves of disposable dishes. Follow the directions on the agar (50 grams per liter of water), sterilize the mix for 40 minutes and then let it cool just enough so the solution is still liquid. Pour a little bit in each dish and then let it solidify. If you have syringes, squirt a fraction of a ml into each dish and swirl the dish around. If you have prints, use a sterile loop, or wire or even a scalpel and scratch a bit of spores onto the knife blade - then smear the spores onto the surface of the agar. Then wait.
 
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