Look for roots coming out of the bottom as an indication to transplant, another indication is you having to water them frequently meaning once a day or so which tells you the roots are filling up the pot and are drinking quick. I grow full organic using no bottled nutes so I would transplant to a 3 gallon then right before flower up to 5 or 7 but if you just want to make 1 transplant definitely go up to 5 right away