Is It Possible
- Thread starter dwcannan
- Start date
Blackhash
Active Member
Very well possible, its called Cross Breeding.
It's actually how Albino Penis Envy came as a strain. It is PF albino and Penis Envy cross breeded together. APE also drops clear spores, kinda cool
It's not a simple process though, and it requires that you have vast knowledge on how to use agar.
Basically, you isolate a single spore on agar and let it colonize then use that to inoculate a jar of substrate.
This mycelium blob will be unable to fruit, mind you.
Then once the jar is about 90% colonized, you must add the spores from the strain you wish to cross, once thats finished colonizing, you fruit it.
And that's the strain you created, but there's no use unless you preserve it.
So let some mushrooms fully mature, then spore print it and make a syringe, and there you go, your own strain!
I wish to one day cross breed some strains, but that would be WAAY in the future.
It's actually how Albino Penis Envy came as a strain. It is PF albino and Penis Envy cross breeded together. APE also drops clear spores, kinda cool
It's not a simple process though, and it requires that you have vast knowledge on how to use agar.
Basically, you isolate a single spore on agar and let it colonize then use that to inoculate a jar of substrate.
This mycelium blob will be unable to fruit, mind you.
Then once the jar is about 90% colonized, you must add the spores from the strain you wish to cross, once thats finished colonizing, you fruit it.
And that's the strain you created, but there's no use unless you preserve it.
So let some mushrooms fully mature, then spore print it and make a syringe, and there you go, your own strain!
I wish to one day cross breed some strains, but that would be WAAY in the future.
Bwpz
Well-Known Member
After my first grow I'm going to get an agar kit, I like the idea of it, the fact that you can grow a little culture and just throw it in the grain without needing any needles or anything.Very well possible, its called Cross Breeding.
It's actually how Albino Penis Envy came as a strain. It is PF albino and Penis Envy cross breeded together. APE also drops clear spores, kinda cool
It's not a simple process though, and it requires that you have vast knowledge on how to use agar.
Basically, you isolate a single spore on agar and let it colonize then use that to inoculate a jar of substrate.
This mycelium blob will be unable to fruit, mind you.
Then once the jar is about 90% colonized, you must add the spores from the strain you wish to cross, once thats finished colonizing, you fruit it.
And that's the strain you created, but there's no use unless you preserve it.
So let some mushrooms fully mature, then spore print it and make a syringe, and there you go, your own strain!
I wish to one day cross breed some strains, but that would be WAAY in the future.
Blackhash
Active Member
Yes agar is definitely something to look into 
I'm ordering a pack of 10 syringes off mushbox just so I have more than 2, because as of right now, I cant do a LC because both my syringes have spores in them
.
And here is a nice agar kit : http://www.mushbox.co/pour_agar_plate.html , if you type the discount code shroomtalk you get $5 off which narrows the total down to $19.99.
I'm ordering a pack of 10 syringes off mushbox just so I have more than 2, because as of right now, I cant do a LC because both my syringes have spores in them
.And here is a nice agar kit : http://www.mushbox.co/pour_agar_plate.html , if you type the discount code shroomtalk you get $5 off which narrows the total down to $19.99.
Bwpz
Well-Known Member
Yeah I was gonna get that one, their shipping is outrageous lol. And thanks for that coupon, that'll helpYes agar is definitely something to look into
I'm ordering a pack of 10 syringes off mushbox just so I have more than 2, because as of right now, I cant do a LC because both my syringes have spores in them
And here is a nice agar kit : http://www.mushbox.co/pour_agar_plate.html , if you type the discount code shroomtalk you get $5 off which narrows the total down to $19.99.
I've got 2 syringes but they'll both be used by my tubs and I'm gonna inject 4 LC's, 2 of each.
Blackhash
Active Member
How are you doing your LC's?
The reason I cant do one is because it will create a vacuum if you just stick a syringe in there and start sucking it up.
The way to get rid of a vacuum is to use an empty syringe with the plunger removed, stick it in there, put the plunger in, then suck up a syringe full of LC.
The reason I cant do one is because it will create a vacuum if you just stick a syringe in there and start sucking it up.
The way to get rid of a vacuum is to use an empty syringe with the plunger removed, stick it in there, put the plunger in, then suck up a syringe full of LC.
dwcannan
Active Member
damn well that dont seem like its all that hard i woulda thought it would have been way more complicated well i guess it is since idk what agar is yet and yeah those albino penis eny's are the shit i tried some im clearwater with my friends brother and we both were trippin balls for like twelve house of just one good size one
Blackhash
Active Member
Agar is using petri dishes with agar substrate on it to colonize with myc.
Lets say you do a bulk grow, and there is a monster fucking shroom on there, you can isolate that shroom and put it on agar, and you get some myc of that shroom.
So when you fruit the sub that you got from that monster shroom, you get some more monster shrooms.
Of course, it can be expanded on and before you really learn agar I suggest you either start with the PF tek or just go straight to grain.
Lets say you do a bulk grow, and there is a monster fucking shroom on there, you can isolate that shroom and put it on agar, and you get some myc of that shroom.
So when you fruit the sub that you got from that monster shroom, you get some more monster shrooms.
Of course, it can be expanded on and before you really learn agar I suggest you either start with the PF tek or just go straight to grain.
Bwpz
Well-Known Member
Would my method of injecting my LC's with microspores work?
Blackhash
Active Member
I was researching LC's and came to the conclusion that you only need 1-2 cc of MS. So in reality you could use 1 syringe, do 8 jars MS inoculation, then do 2 LC's and have an empty syringe to inject other jars with said LC's. But, if you're trying to get 2 LC's of different strains, you could leave 1 cc in one syringe, then inject the other LC with 1cc of the other strain. But then you would either have to trash the rest of the MS syringe, or just inject another grain jar.
Bwpz
Well-Known Member
That was my plan
8 jars of APE and 2 accompanying LC's, and then 8 jars of PF Albino and 2 LC's for that.
canndo
Well-Known Member
You can dilute your spore solution - until there are very few spores 10 to 20 per ml. Innoculate your dish and then lift each colony out and place it in it's own dish. Then you can experiement with joining a bit of one colony with a bit of another and grow the results out. If you had a microscope that resolves fine enough you could check for clamp connections. Evidence of clamp connection usually indcates that you have a dikaryotic mycelial mat (it will likely fruit). You can also exchange genetic information from one dikaryotic mat to another and form a new strain that way. This is where agar works well as you can see the results in the sectoring of your mycelia. You can also place a few different types of spores on a dish and look for the most powerful sector, take a bit of that sector and transfer it to another dish. Odds are if you do that several times you will get a new mushroom type (not necessarily a new strain). This is a good way to get yourself a host of mushroom types with very little work - then you can grow out each type and see what characteristics each has.