Hal Incandenza
Active Member
[The following info is from High Times #422, p.64]
Micro cloning is sort of like regular cloning, but done sterile and on a minature scale in jars. Its quickly becoming the way that most clones are made and genetics are kept, which is why you should read this.
As an example; Almost all the plant at a garden shop are TC
Think limitless numbers of plants that are identical, but without the huge mother rooms and problems that were used to.
The process in a nut-shell, is this: Take small tip cuttings from a mother plant and wash them with soap and water, then alcohol, then diluted bleach water. These cleaned tips are then put in a special gelatiin mix in small jars to grow without roots as a micro bonsai. ''What special gelatin?'' It comes in TC kits: officially, its purified water,standard nutrient salts, vitamins and amino acids, branching hormone, peservative, and gellan gum to make it all gelatinous.
Every five weeks or so, the cultures will grow from one node to approximately five and then are cut apart and put into five new jars. This is called the multiplication stage: repeat until you have your desired amount. When you need clones you siply cut apart as before but use rooting hormone instead of branching hormone and wait two weeks. At that point transfer to standard clone tray with grow medium. now you have a whole tray of rooted clones.
PROS:
*Keep 100 stains under a single t5, and only have to rejar every 5-8 weeks.
*no more watering.
*Genetic drift is eliminated, Even damaged genetics can be brought back to their origanal vigor.
*Less watage for mothers.
*Bugs and mold are no longer.
*There is no other practical way to provide high numbers of clones that are all short with a high node count and at the same stage of growth.
CONS:
*You have to plan 5 weeks ahead, versus 2 for traditional cloning.
*Theres a 10 week initial lead time to deal with.
*If you have a aversion to being clean you may have some problems.
You can buy kits for this.
------------------------------
Here are some TC kits I found:
1. http://www.led-grow-master.com/PlantTissue.html
2. http://www.bghydro.com/BGH/itemdesc.asp?ic=PRAPTCCK
------------------------------
Micro Propagation Video
-----------------------------
Does anyone here use this method?
Micro cloning is sort of like regular cloning, but done sterile and on a minature scale in jars. Its quickly becoming the way that most clones are made and genetics are kept, which is why you should read this.
As an example; Almost all the plant at a garden shop are TC
Think limitless numbers of plants that are identical, but without the huge mother rooms and problems that were used to.
The process in a nut-shell, is this: Take small tip cuttings from a mother plant and wash them with soap and water, then alcohol, then diluted bleach water. These cleaned tips are then put in a special gelatiin mix in small jars to grow without roots as a micro bonsai. ''What special gelatin?'' It comes in TC kits: officially, its purified water,standard nutrient salts, vitamins and amino acids, branching hormone, peservative, and gellan gum to make it all gelatinous.
Every five weeks or so, the cultures will grow from one node to approximately five and then are cut apart and put into five new jars. This is called the multiplication stage: repeat until you have your desired amount. When you need clones you siply cut apart as before but use rooting hormone instead of branching hormone and wait two weeks. At that point transfer to standard clone tray with grow medium. now you have a whole tray of rooted clones.
PROS:
*Keep 100 stains under a single t5, and only have to rejar every 5-8 weeks.
*no more watering.
*Genetic drift is eliminated, Even damaged genetics can be brought back to their origanal vigor.
*Less watage for mothers.
*Bugs and mold are no longer.
*There is no other practical way to provide high numbers of clones that are all short with a high node count and at the same stage of growth.
CONS:
*You have to plan 5 weeks ahead, versus 2 for traditional cloning.
*Theres a 10 week initial lead time to deal with.
*If you have a aversion to being clean you may have some problems.
You can buy kits for this.
------------------------------
Here are some TC kits I found:
1. http://www.led-grow-master.com/PlantTissue.html
2. http://www.bghydro.com/BGH/itemdesc.asp?ic=PRAPTCCK
------------------------------
Micro Propagation Video
-----------------------------
Does anyone here use this method?
