papablunt
Active Member
I stumbled upon this extremely tantalizing article....which is linked below....and below that is the actual process from the article....but the article is way more amusing. Genetically engineered food gives me the creeps, but I may have to make an exception now. Gives new meaning to orange crush.
http://sttf.org/wires/1998/11/09/oranges-that-get-you-high/
Biochem 101: How to design a Cannabis-equivalent citrus plant
Step One:
Biochemically isolate all the required enzymes for the production of THC.
Step Two:
Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes.
Step Three:
Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium.
Step Four:
Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited.
http://sttf.org/wires/1998/11/09/oranges-that-get-you-high/
Biochem 101: How to design a Cannabis-equivalent citrus plant
Step One:
Biochemically isolate all the required enzymes for the production of THC.
Step Two:
Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes.
Step Three:
Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium.
Step Four:
Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited.
