Tissue Culture

born2killspam

Well-Known Member
That is a nice link.. One thing it didn't mention though that can be greatly helpful is working in proximity to a flame like a torch/candle when transferring sterilized tissue to the sterile culture.. The heat created a massive up-flow that helps keep any airborne pathogens from falling/collecting on surfaces nearby.. Ontop of that, a diy Pasteur flask will help prevent infection during the growth phase.. Not that tough to rig something similar up.. I found these practices extremely helpful when I was culturing yeast sources..
 

coconutbeach

Well-Known Member
Or it can be done with no flame at all and just soaking the instruments in 70% isopropyl alcohol from drug store. I found the rising air in the storage tub could draw in cooler contaminated air from the lower front side across the clean surfaces, and it aviods heat and hot instruments.
 
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