skunkushybrid
New Member
Finally got a bonafide root on my leaf clone. As soon as it takes properly I'm going to put it in a little pot and grow it alongside the others.
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wow, didn't figure on such an amazing response.
I did it exactly the same way as I clone normally. Soaked the rockwool overnight in plain water (ph'd to 5.5, as this takes the alkalinity out of the rockwool), sprayed the leaf twice a day with luke warm water, also ph 5.5. I keep the water luke warm by leaving it close to the light. Usual diagonal slice across the stem. I also like to groove the tip of the stem, but on saying that I don't think it makes much difference.
The cloning gel I use is really cheap. I buy them in a pack of six, usually you are meant to place a cutting straight into a pot... but I empty all the pots into a larger container and use this as my rooting gel. It's by a company called gel2root. 2.99 (pounds) for six pots. I can't see myself ever needing to buy rooting gel again. Each pot contains 125ml of rooting gel.
Thats what I was going to suggest. Tissues cultures come from leaf discs anyway. You could maybe try using or copy the ingredient in tissue culture agar too.I think I may be able to help on this one, the addition of gibberellic acid and kinetin should be able to force some of the leaf's cells to differenciate into actual growth tips. The application may need to be very precise with the use of soaked gauze wraped around the stem in order to force it to penetrate the cells but it should be posable. These hormones are commonly used in plant tissue culture for helping the plant grow out of a callus, the callus often being taken from leaf material. I have both hormones so I will try this out and get back to you guys... oh and they are cheap chemicals so maybe I can figure out the recipe for everyone else.