Plant tissue culturing

[TerrapinBlazin]

This is my first post in this section, and I wanted it to be about something more “advanced” than low stress training.

My background is in microbiology/mycology. I made the switch from mushrooms to weed because I don’t have enough space for a proper lab and too much spawn was getting contaminated and ruining my yields. Anyway, I digress.

I still have all my culturing gear, including a custom built (by yours truly) 2x2 foot laminar flow HEPA cabinet, and right now it’s all mothballed. I would love to use it again, hence my interest in tissue culturing. My understanding is that any sample of plant tissue is capable of producing roots and meristems under the right conditions, and I’m just interested in a little more info. If anyone has some instructional material that would point me in the right direction, that’s all I really want.

I’ve known how to culture on agar since high school. All the lab work is pretty easy for me, but I don’t know anything about the nutrient media or incubation process. I know this is a kind of “out there” thing to post a question about, but I’m only asking because I’m already equipped to do it. Thanks in advance.

 

[Chip Green]

Do a search, for tissue culture posts from the member @SCJedi
Something I'm planning on delving into in the near future, as I also have experience in freelance mycology, back before the turn of the century....

 

[TerrapinBlazin]

Nice. Yeah I’m sure I can do it with the flow hood, and what I’m most interested in is slanting/tubing cultures of my best phenos for long term storage like I have with my mushroom cultures. I don’t know if that’s doable, but tissue cultures take up a lot less space than mother plants. Archiving killer phenos with minimal senescence would obviously be the main goal, but I’m interested to see how it works for propagation, or for a backup cloning method if a cutting fails and the only specimen is flowering. I don’t have room in my tent to re-veg anything so this would be awesome for keeping a library of the best phenos and salvaging the ones that would otherwise be gone after flowering.

 

[hexthat]

these four little explants came from a 4 oz canning jar. keeping it small for that cold storage for 8 months.... trying to lower HLVd

MS Cannabis Multiplication Basal Salt Mixture with meta-Topolin

MS Cannabis Multiplication Basal Salt Mixture with meta-Topolin

phytotechlab.com

this is pre made stuff without agar

 

[xtsho]

these four little explants came from a 4 oz canning jar. keeping it small for that cold storage for 8 months.... trying to lower HLVd

View attachment 4521744

MS Cannabis Multiplication Basal Salt Mixture with meta-Topolin

MS Cannabis Multiplication Basal Salt Mixture with meta-Topolin

phytotechlab.com

this is pre made stuff without agar

I've been looking into tissue culture for awhile. I just haven't had the time to do enough research before I start working with it. I did have this link to PhytoTech Labs bookmarked but I see you're not using one of the kits they offer. That's why I need to do more research as I don't want to buy equipment and supplies I won't need. I've been wanting to dabble in this for awhile. Just not for cannabis but for other plant species as well. Neat stuff.

Kits

PhytoTech Labs is a leading manufacturer of plant tissue culture media with equipment, supplies, and components available to support plant production and research. An experienced team in customer and technical service backs every interaction and product. Our refined manufacturing processes...

phytotechlab.com

 

[hexthat]

I've been looking into tissue culture for awhile. I just haven't had the time to do enough research before I start working with it. I did have this link to PhytoTech Labs bookmarked but I see you're not using one of the kits they offer. That's why I need to do more research as I don't want to buy equipment and supplies I won't need. I've been wanting to dabble in this for awhile. Just not for cannabis but for other plant species as well. Neat stuff.

Kits

PhytoTech Labs is a leading manufacturer of plant tissue culture media with equipment, supplies, and components available to support plant production and research. An experienced team in customer and technical service backs every interaction and product. Our refined manufacturing processes...

phytotechlab.com

Kits are good if you have the money for them. I do everything as ghetto and low budget as possible.

 

[xtsho]

Kits are good if you have the money for them. I do everything as ghetto and low budget as possible.

I'm the king of low budget. That's why I haven't rushed to buy a kit. That and I too many other projects going on right now. But tissue culture is something I will do in the near future.