HpLVd, information, Vendors, Testing and Reviews

sunsetdaydreamer

sunsetdaydreamer

Well-Known Member
I guess considering most sellers are NOT testing, shall we make a list of sellers who definitely Are testing and perhaps putting test results to those who we know definitely have got hplvd..?
I started following a guy on Instagram today called
Joesramahi. I know he offers a strain clean up service but I've not messaged him at all he also seems to focus on hplvd
This is his Instagram account

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Also...

Nodelabs
Dark heart
Black sheep farms

All do tissue culture and offer services to remove viruses from clones. So if you have anything valuable that you don't want to lose forever, I'd contact those or if you know of any labs who provide the service list any others here in this thread..
 
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LGND

Well-Known Member
Here's a Gorilla Butter from Pink Box Clones that tested positive for HPLVD. My other clone stock all tested negative. I'm taking a tissue culture class to hopefully setup my own lab to refresh all my genetics in the future. From what I've seen the only way to cure HPLVD is to perform a hot treatment on a plant for a month then take a tissue culture via the meristem.



 
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LGND

Well-Known Member
I use an Aero cloner for my clones and asked dark heart about transmission and this is their response:

"It has a very low possibility if not zero possibility of transmitting through an aero cloner. If there is a wound sight, that is the low possibility. I asked our scientist and he agreed. Hplvd is transmitted through wound sites and puning is the largest known cause. "
 
sunsetdaydreamer

sunsetdaydreamer

Well-Known Member
Yeah but most likely would use the same scissors for them. And I also read sap being how it transmits which I would think would be in the cloner water
 
sunsetdaydreamer

sunsetdaydreamer

Well-Known Member
LGND said:
Here's a Gorilla Butter from Pink Box Clones that tested positive for HPLVD. My other clone stock all tested negative. I'm taking a tissue culture class to hopefully setup my own lab to refresh all my genetics in the future. From what I've seen the only way to cure HPLVD is to perform a hot treatment on a plant for a month then take a tissue culture via the meristem.



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Definitely has that flat outward growth thing
Thanks for the picture
 
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LGND

Well-Known Member
sunsetdaydreamer said:
Yeah but most likely would use the same scissors for them. And I also read sap being how it transmits which I would think would be in the cloner water
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Cleaning scissors between each plant should be standard practice for people wanting to try and avoid the virus. 70% Iso alcohol minimum but 10% bleach solution would be best.

For the sap I really can't say. That's one experiment I don't want to try lol.
 
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Mattbryson

Well-Known Member
LGND said:
Cleaning scissors between each plant should be standard practice for people wanting to try and avoid the virus. 70% Iso alcohol minimum but 10% bleach solution would be best.

For the sap I really can't say. That's one experiment I don't want to try lol.
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If you don't mind whats dark heart charge per test? I just sent them a email wouldn't mind getting my mothers tested just for peace of mind ? Thanks for your time
 
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Mattbryson

Well-Known Member
LGND said:
They charge $25 per sample. I sent out my samples with cold packs just in case to keep them fresh. Turn around time was two business days from when they received the samples.
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Thanks for the response definitely going send samples in to get a clean bill of health i stop buying cuts cause all the stuff going around seems everyone has a dirty cut in there garden crazy
 
sunsetdaydreamer

sunsetdaydreamer

Well-Known Member
LGND said:
Cleaning scissors between each plant should be standard practice for people wanting to try and avoid the virus. 70% Iso alcohol minimum but 10% bleach solution would be best.

For the sap I really can't say. That's one experiment I don't want to try lol.
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Well I guess now if you start collecting then yes would definitely do that although I'm sure I've read that leaf to leaf contact can also spread it.
 
sunsetdaydreamer

sunsetdaydreamer

Well-Known Member
LGND said:
Here's a Gorilla Butter from Pink Box Clones that tested positive for HPLVD. My other clone stock all tested negative. I'm taking a tissue culture class to hopefully setup my own lab to refresh all my genetics in the future. From what I've seen the only way to cure HPLVD is to perform a hot treatment on a plant for a month then take a tissue culture via the meristem.



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Apical Meristem culture is for disease or virus removal. I'm not sure if regular Meristem culture is enough to get clean cultures. Ive never heard of hot treatments but I've read studies on cold treatments plus virozole.
 
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xtsho

xtsho

Well-Known Member
Easy solution. Just don't get the so called "Great Cuts". If they're so great then why do they carry a pathogen?

Screw cuts, the latest and greatest breeders, seed drops, etc... I'll stick to growing seeds of landrace strains, make my own crosses, and grow great cannabis without dealing with any of this soap opera drama.

I don't bring any foreign clones into my grow. Everything starts from seeds I sprout.
 
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Applesauceisgood

Well-Known Member
Tangerine_ said:
I'd think just a little guttation would be enough to vector it leaf to leaf.
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I doubt it. A virus would most likely be present in the phloem and not in xylem sap. Aphid stylets are used to puncture the phloem and it's a way transfer can occur, however, literature I've read indicates aphid transfer is not taking place. I think plant-to-plant transfer would require a cut or puncture of the stem or petiole to then contaminate an open wound of a neighboring plant. Plants are typically able to seal wounds quickly. Plants brushing up against each other repeatedly with a force such as a fan might facilitate the transfer. Transfer of phloem sap via mechanical cutting tools lacking proper disinfection seems to be the main cause for concern. Something I point out is household bleach is typically sub 10% sodium hypochlorite. People need to pay attention to how long tools are dipped and at what solution strength. I suspect people are quickly dipping their pruning tools in 6% bleach and calling it a done deal. This lack of attention to detail is why the virus will continue to spread, even among many vendors claiming to be clean.
 
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Applesauceisgood

Well-Known Member
xtsho said:
Easy solution. Just don't get the so called "Great Cuts". If they're so great then why do they carry a pathogen?

Screw cuts, the latest and greatest breeders, seed drops, etc... I'll stick to growing seeds of landrace strains, make my own crosses, and grow great cannabis without dealing with any of this soap opera drama.

I don't bring any foreign clones into my grow. Everything starts from seeds I sprout.
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The virus isn't an enigma. Great cuts are called as such because they have typically been vetted for many years by respected people and found to have archival qualities. Sourcing a vetted cut and spending $50 for labwork is more efficient than popping hundreds or thousands of seeds to find a plant that might possess half the desirable traits. It isn't logical to disregard cuts that displayed elite traits, which were potentially maintained for decades because poor grower practices lead to pathogen contamination. If you can't take basic precautions when acquiring outside genetics, searching for a win over a lifetime of seed popping is the way to go.
 
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xtsho

xtsho

Well-Known Member
Applesauceisgood said:
The virus isn't a mystery. Great cuts are called as such because they have typically been vetted for many years by respected people and found to have a combination of traits that make them archival. It's more efficient to source a great cut and spend $50 testing it for pathogens compared to popping hundreds or thousands or seeds to find a plant that might possess half the desirable traits. Diminishing cuts that displayed elite traits then maintained for long periods of time because they picked up a pathogen along the way because of poor user practice isn't logical.
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You don't have to pop thousands of seeds to find something great. That's a myth that needs to go away.
 
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Applesauceisgood

Well-Known Member
xtsho said:
You don't have to pop thousands of seeds to find something great. That's a myth that needs to go away.
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Finding the equivalent of ECSD (insert 30 other elites) in seeds resulting from an amateur pollen chucker is winning the lottery. Yes, it can happen but the time, space, and energy dedicated to finding an equal are many times more than the price you'll pay for a sourced + tested "great cut". For little money and a $50 test pathogen test, you can source one of the best plants crowdsourced out of tens of thousands of attempts. How many ghost og or bubba kush have you stumbled upon that captured the attention of thousands of growers and altered what people smoked and bred with for multiple decades? I understand the fun and reward of seed popping but it's a silly debate. It's only winnable if you muddy the waters by implying great cuts aren't great because they are contaminated and you don't want to pay the penny to eliminate the risk. I can also agree if you're approaching it in terms of seeking a niche effect not found in many elites.
 
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xtsho

xtsho

Well-Known Member
Applesauceisgood said:
Finding the equivalent of ECSD (insert 30 other elites) in seeds resulting from an amateur pollen chucker is winning the lottery. Yes, it can happen but the time, space, and energy dedicated to finding an equal are many times more than the price you'll pay for a sourced + tested "great cut". For little money and a $50 test pathogen test, you can source one of the best plants crowdsourced out of tens of thousands of attempts. How many ghost og or bubba kush have you stumbled upon that captured the attention of thousands of growers and altered what people smoked and bred with for multiple decades? I understand the fun and reward of seed popping but it's a silly debate. It's only winnable if you muddy the waters by implying great cuts aren't great because they are contaminated and you don't want to pay the penny to eliminate the risk. I can also agree if you're approaching it in terms of seeking a niche effect not found in many elites.
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I don't believe in these "Elite" strains. There are plenty of others making and popping great stuff from seed. They're just not these "Known" breeders with connections winning some "Cup". I've grown stuff from seed as good or better than anything I've come across in the 4+ decades I've been smoking weed.

I've never bought in to the hype. Just recently I got a call from a guy I gave some plants to. Some of them came from seeds of my own pollen chucks. The guy was livid. He wanted to know what it was he was growing because it was so great. He didn't seem satisfied when I told him it was just a pollen chuck I made. He wanted to hear that it was some "X" strain from some super "X" breeder. He seemed disappointed that it wasn't.

Who wants a great strain from a nobody like me? It's not pre-1990 super Smash Bling Monkey Skunk from Billy Big Banger Best breeder Ever. You can't brag to your buddies.

The cannabis strain/breeder scene is one big soap opera I don't need and want nothing to do with. I'll still be growing and smoking weed as good or better without worrying about that world. Cartoon and Candy names and packaging is something I have no need for.
 
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