Anybody growing mushrooms willing to talk???

Aeroknow

Well-Known Member
If they are in the sheath and rad sterilized they are just fine. I don't sterilize further, no flame, no antiseptic. It's all in technique. Rapidity, isolation and redundancy. One needle, one syringe, one jar or bag.
I feel really good about my technique. I’ll do a round here without sterilizing the brand new sterilized needles. Or try to one of these days It’s been very hard to allow myself to do that. Don’t wanna contam a jar. Maybe i’ll do like the last 1/3 of a quart? But def aint trusting that on a master.
I do however inject 3 of my grain bags with one 30cc syringe and needle and I don’t sterilize the needle in between bags. This is in front of a flow hood. Took me a minute to trust myself to do that but it works fine. I hardly ever contaminate any of my grain bags and I pump out allot.
 

Aeroknow

Well-Known Member
Yeah, those are silly.
Hey they do work. He was gonna wait til they colonized and then dump em into a tub if i remember correctly.
I grow in bags. Everyone does now. It’s how all the bulk grows are done now. I make my own though for pennies. I make everything. Actually for about 7 dollars a pound. I will argue that mono tubs are outdated but i’m also worried about all the waste i’m making and also tried to make tubs work.
 

Fangthane

Well-Known Member
Hey they do work. He was gonna wait til they colonized and then dump em into a tub if i remember correctly.
Yeah, that was the plan. At least someone is paying attention. They worked great for me the first time around; just managed to strike out this time. But I guess it was just a silly idea to start. :rolleyes:
 

Aeroknow

Well-Known Member
Yeah, that was the plan. At least someone is paying attention. They worked great for me the first time around; just managed to strike out this time. But I guess it was just a silly idea to start. :rolleyes:
My money is on the fact that you shot em up with ms syringe. At least the two of them. If you shot em up with LC you would have been good. Just like i had said about offering some JF spawn if you lived here in NorCal i’d kick you down some super potent syringe of the JF lc. Like, hard to inject cause it’s so thick.
Cant really be certain that you shot up some spores, let alone compatible spores.
there is a chance that MAYBE the grain was too wet in the all in one bag but unlikely because the bulk substrate in there would most likely keep the grain at a decent moisture level. Unless it too was too wet.
Not sure how they make the all in one bags but most companies make their grain bags in a no soak no simmer fashion. You really need to mix that shit up to redistribute the moisture within the grains before you knock it up. That is the easy way, which is what i do with my grain for spawn so i’d imagine thats what they do? They dump a measured(weighed)amount of grain. Then water for that grain then they dump some field capacity bulk substrate in there? Then autoclave?
 
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Fangthane

Well-Known Member
My money is on the fact that you shot em up with ms syringe. At least the two of them. If you shot em up with LC you would have been good. Just like i had said about offering some JF spawn if you lived here in NorCal i’d kick you down some super potent syringe of the JF lc. Like, hard to inject cause it’s so thick.

there is a chance that MAYBE the grain was too wet in the all in one bag but unlikely because the bulk substrate in there would most likely keep the grain at a decent moisture level. Unless it too was too wet.
If anything, the grain level seemed dry to me, at least compared to the first bags I used that worked out fine. They also seemed fairly loosely packed. Like a loose granular mass of grains as opposed to something that was a moist cohesive block. Shitty explanation; just can't think of any better way to describe. MS syringes are all I've ever had access to, and didn't seem to have kind of negative impact on my first run. No idea. Maybe I did manage to do something magnificently stupid without realizing it, but can't think of a single thing I did appreciably different this time around. Guess I'll have to fully rethink my next attempt. I think it'd be pointless for a once a year grower to buy a pressure cooker and the various ingredients on hand, and my first run told me that the AIO bags are anything but silly. Just didn't work out this time for whatever reason.
 

Aeroknow

Well-Known Member
If anything, the grain level seemed dry to me, at least compared to the first bags I used that worked out fine. They also seemed fairly loosely packed. Like a loose granular mass of grains as opposed to something that was a moist cohesive block. Shitty explanation; just can't think of any better way to describe. MS syringes are all I've ever had access to, and didn't seem to have kind of negative impact on my first run. No idea. Maybe I did manage to do something magnificently stupid without realizing it, but can't think of a single thing I did appreciably different this time around. Guess I'll have to fully rethink my next attempt. I think it'd be pointless for a once a year grower to buy a pressure cooker and the various ingredients on hand, and my first run told me that the AIO bags are anything but silly. Just didn't work out this time for whatever reason.
If only doing it once a year, promise me you’ll only buy an LC syringe/s . Message me if you’re looking for a trusted site to buy from.
 

canndo

Well-Known Member
Hey they do work. He was gonna wait til they colonized and then dump em into a tub if i remember correctly.
I grow in bags. Everyone does now. It’s how all the bulk grows are done now. I make my own though for pennies. I make everything. Actually for about 7 dollars a pound. I will argue that mono tubs are outdated but i’m also worried about all the waste i’m making and also tried to make tubs work.
First, you can't shake the bag which lengthens colonization time. Second, you can't signal the mycelium that it is running out of nutrient. You can't level the substrate and then level the casing on top. It's pretty haphazard and uncontrolled. I have no problem with bags at all. But these sorts of things defeat the whole growing cycle.
 

Aeroknow

Well-Known Member
First, you can't shake the bag which lengthens colonization time. Second, you can't signal the mycelium that it is running out of nutrient. You can't level the substrate and then level the casing on top. It's pretty haphazard and uncontrolled. I have no problem with bags at all. But these sorts of things defeat the whole growing cycle.
Not too sure where you’re going with this. But if you’re referring to what i said earlier about some of my grain bags lagging behind, i’m pretty sure its a distribution of moisture thing in my bags. I do a no soak no simmer with a milo and millet combo. You MUST shake up the bags hella good, while still hot from the PC, in order to redistribute the moisture. Some batches i end ip letting cool over night before opening and mixing them up. Which is allot harder to do. It is usually gonna be some of those bags that some of them lag behind the others. Once i mix them up again they take off.
like i had said, i hardly lose any bags to contamination.
 

canndo

Well-Known Member
Not too sure where you’re going with this. But if you’re referring to what i said earlier about some of my grain bags lagging behind, i’m pretty sure its a distribution of moisture thing in my bags. I do a no soak no simmer with a milo and millet combo. You MUST shake up the bags hella good, while still hot from the PC, in order to redistribute the moisture. Some batches i end ip letting cool over night before opening and mixing them up. Which is allot harder to do. It is usually gonna be some of those bags that some of them lag behind the others. Once i mix them up again they take off.
like i had said, i hardly lose any bags to contamination.

I was talking about the pre-made bags with the dirt on top.

That you can't shake them after they are innoculated.
 

natureboygrower

Well-Known Member
If they are in the sheath and rad sterilized they are just fine. I don't sterilize further, no flame, no antiseptic. It's all in technique. Rapidity, isolation and redundancy. One needle, one syringe, one jar or bag.
I ran your jar method two times, 24 1-quart jars, and only had one fail. I pretty much followed all your cleaning and sterilizing steps ( including vacuuming 24 hours or so ahead of time) followed by lysol spraying the room. A lot of comments in that thread about overkill, but it worked great for me and sometimes it's easy to overlook smaller things that matter.
 

canndo

Well-Known Member
I ran your jar method two times, 24 1-quart jars, and only had one fail. I pretty much followed all your cleaning and sterilizing steps ( including vacuuming 24 hours or so ahead of time) followed by lysol spraying the room. A lot of comments in that thread about overkill, but it worked great for me and sometimes it's easy to overlook smaller things that matter.

I'm glad, remember the method is only for first time application and not for any sort of bulk. I may post one of those eventually.
 

natureboygrower

Well-Known Member
I'm glad, remember the method is only for first time application and not for any sort of bulk. I may post one of those eventually.
I was curious whether or not you could inoculate in the jars then do the shoeboxes like these guys are doing. Guess that answers that.
The jar method worked great, almost too good. Those jars of mine grew mushrooms for almost 3 months, which would be my only complaint. But perfect for someone who wants fresh mushrooms every week.
 

Aeroknow

Well-Known Member
I was talking about the pre-made bags with the dirt on top.

That you can't shake them after they are innoculated.
Ah! He might have just been taking the cakes out of the bag and putting them in tub/s. Or keeping the cakes in the bag and cutting off the rest?
 

Aeroknow

Well-Known Member
Sure, but the dirt was in there. Why bother?
I’ve never done an all in one bag but the way i understand you do it is once the grain is colonized you mix it all together with the bulk substrate. I’ve seen people(online)take that then fully colonized cake and do all sorts of stuff with it, like putting it into a monotub, besides just fruiting in the bag.
In a small setup It is a pretty safe way to get to fruiting as long as the Lc and the needle is clean :-)
 

canndo

Well-Known Member
I’ve never done an all in one bag but the way i understand you do it is once the grain is colonized you mix it all together with the bulk substrate. I’ve seen people(online)take that then fully colonized cake and do all sorts of stuff with it, like putting it into a monotub, besides just fruiting in the bag.
In a small setup It is a pretty safe way to get to fruiting as long as the Lc and the needle is clean :-)
The whole point is to expand the mycelium in the cheapest, fastest and safest way possible.

Now, there is no better way than monoculture. This means you can Taylor all your methods to a single "phenotype".
The organism will fruit in predictable ways and for a predictable number of flushes.

You also want the entire system to yield its entire potential in the fewest number of flushes.

You want to get away from sterility as quickly as possible because sterility is the antithesis of "natural".

The more exotic the mixtures the more difficult you make it for no real reason.

Check your variables.

Light
Moisture
Temperature
Ph

Ph is the most under managed but is the key to ongoing successful large scale grows.

Manipulation of light is more important than anything but ph.

Anyone, ANYONE can grow a large amount of this species.... once or twice.

The skill is in being able to repeat the process ongoingly.
 
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Aeroknow

Well-Known Member
The whole point is to expand the mycelium in the cheapest, fastest and safest way possible.

Now, there is no better way than monoculture. This means you can Taylor all your methods to a single "phenotype".
The organism will fruit in predictable ways and for a predictable number of flushes.

You also want the entire system to yield its entire potential in the fewest number of flushes.

You want to get away from sterility as quickly as possible because sterility is the antithesis of "natural".

The more exotic the mixtures the more difficult you make it for no real reason.

Check your variables.

Light
Moisture
Temperature
Ph

Ph is the most under managed but is the key to ongoing successful large scale grows.

Manipulation of light is more important than anything but ph.

Anyone, ANYONE can grow a large amount of this species.... once or twice.

The skill is in being able to repeat the process ongoingly.

So, moving steadily from a mere few strands of mycelium to a usable spawn to a large amount of a specific lot of a general bulk material and initiating fruiting conditions as quickly as possible is how it all works together.

Orchestrating that pinning sequence is how you get the very highest yield in the shortest time with the least amount of energy or money expended.

Straw and or straw manure mixes are the most efficient with cubes, bar none.

Substrate depths of 8 to 12 inches are optimum but they carry huge risks that must be managed.

With this combination and judicious use of water crystals, one can get the most on a per square foot (not cubic foot as surface area is all that growers are really concerned with).

In this way you can completely expend the nutrients in the substrate in two flushes. Then the spent substrate is turned out and the process is repeated. This means the substrate and casing never gets to an acidic state. If it never gets to a stage that enables trich growth, no contamination occurs.

The species doesn't really care about continuing fresh air, so long as there is no stagnant air and the co2 levels never get beyond 5000 ppm, fruit will be abundant. Good lighting will encourage dense pin sets and deep nutritional availability will guarantee few if any aborts.

Bags, jars, bins, trays, buckets, these are non essentials. People get hung up with the mechanics, they never have to with this species.
My newest cube variety that i’m in love with is Gandalf and i grow allot of different cubes. 80g’s dried from first flush in an XLS bag. That’s a solid 1/2lb in a monotub first flush. 24 days from spawn to bulk. I only do first flushes because of efficiency. I hate monotubs. Every single one of those growbags make it. They make it to a second and a third but i grow more mushies keeping the new bags going in. Like i had said before, i got some kind of spore load going on in my old bud flowering room and i’m not alone. Bag growing with LC works great. I have no need to do a grain to grain transfer either to me that’s old tek.
 
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