canndo
Well-Known Member
We all learn as we go.
Anybody growing mushrooms willing to talk???
- Thread starter 7L!fTeD24
- Start date
We all learn as we go.
canndo
Well-Known Member
Yep, we continue to learn.
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natureboygrower
Well-Known Member
Yeah, well I knew it was 10minutes when you originally said 5, because I read and followed the can directions when I sprayed my room down. Which, as an fyi, spraying large amounts of it will keep surfaces wet for 10 minutes. Especially this time of year while it's cold.
I'm not pushing lysol and i will take your word on the bleach.
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MintyDreadlocks
Well-Known Member
Big ol' chonker of Mexico Mazatapec. She sure looks dangerous. My Orange Octane girl in the background. (Black Farm Genetix)
38g Wet
38g Wet
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canndo
Well-Known Member
Yeah, 10, not 5. And it also says you shouldn't "saturate" surfaces so I really don't know. Also, it's different for different things... but most of them are bacteria and not spores.
So I really have no idea of how good it is for what we do. I use the lysol wipes in order to feel good about having done something that at least smells clean.
MintyDreadlocks
Well-Known Member
If stored in syringe there is a special luer lok tip that allows for gas exchange. If in a jar it needs to be modified for gas exchange.
canndo
Well-Known Member
But the mycelium is submerged in the liquid. Does it draw from dissolved oxygen? If so, will a failure to agitate it slow growth?
MintyDreadlocks
Well-Known Member
Something along those lines, yeah. I don't think it would slow growth tremendously and in some cases little agitation is better. But you might have uncolonized liquid culture in some spots making your syringe pulls less consistent with living mycelium. Agitating keeps the threads evenly distributed in the liquid solution. Instead of the threads combining and creating that cakey mushroom mass. Think of it like the break and shake method. When you break up your grain bags you distribute that mass of mycelium throughout the substrate creating dozens of inoculation points essentially.
Stirring should only be done once a day to get the fastest growth rates. People who leave them on fancy stir plates for 24/7 are working against themselves and making the process much longer than it needs to be. If I break and shake my bags everyday I'd never have any mushrooms. So seems redundant to stir for 24 hours.
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canndo
Well-Known Member
I am not
Thanks, I am not much worried about that conglomeration effect as I am not growing that particular species. I have noticed that constant stirring serves no purpose but when I have done so it was too slow to have dissolved much oxygen. When I do spin it, I do so to the point of cavitation (well, there are dispursed bubbles anyway). I just wonder if it makes a difference.
Drop That Sound
Well-Known Member
7L!fTeD24
Well-Known Member
I'm thinking about doing this when I get my flow hood. This way, I can just take the lure lock syrinches and screw them on and suck the LC out. Then what I'm done Screw another syringe onto it until i'm ready to draw again.
7L!fTeD24
Well-Known Member
What do you guys think about this? I'm using R TV for my ships on my LC jars And usually when I've been drawing out of them I put a piece of micropore tape over the hole in the ship that was created from the hot needle, Instead of doing that now. I just put another dab of R TV over the hole.
7L!fTeD24
Well-Known Member
I like this new lc recipe, used to eyeball a pinch of Lme and only use that. Now I'm using Lme, peptone, and Karo, weighing out the recommended amounts. Seems to have colonized way faster And it is colonizing rapidly at 19 days. Had to put them in the fridge. I wonder if these additives like karo give some legs to the nutrition Allowing the mycelium to be fed longer, in turn keeping the mycelium healthier for a longer period of time. I kind of slacked and didn't swirl my jars at all for about 3 days, And that's when I got some of the white Floaters on top, Which didn't happen before unless I let them sit for a week or two without swirling. So to be safe I do it everyday now. I pulled out of my first jar today and it sucked up surprisingly easy For how Thick the mycelium looks.
7L!fTeD24
Well-Known Member
I restarted everything from scratch, Emptied out the tent, scrubbed it down, tore carpet out, and cleaned the room really good. Then what I did was made a bunch of shoeboxes, the spawn jars looked really good But wanted to keep every one of them isolated trying to narrow down the source of trich. So these shoeboxes should give me an idea of how things are going to go after all the cleaning and if that was the problem at all. Enigma, Pe7, Blue magnolia, Ape, Hillbilly, and Jmf all going. And many more to come if things go right. At this point I'm kind of afraid to put all my eggs in one basket Doing larger monotubs With five quarts of spawn. I may stick with the shoe boxes Until I get a flow hood In a month.
7L!fTeD24
Well-Known Member
Nice bud and boomers. My maz always came out tiny. Yes that plant has me itching to grow some plants l o l. Does anybody have any problems growing Both. I feel like they could definitely cross contaminate Especially because I use a lot of microbial products And organic nutrients. Maybe it would be a loss of a risk using synthetic nutrients.
canndo
Well-Known Member
How big are your syringes?
canndo
Well-Known Member
Don't use hot needles, firing your needles really serves no purpose. Firing scalpels does because you are definitely making contact with mycelium or spores.
Needles are cheap and you can successfully autoclave them over and over again.
We mycological folk learn a super power, you have it I'm sure. You can "see" bacterial and fungal spores in the air.
Not really but you can aquire a sense of what is hanging in the air. You are aware if your operating edges have made physical contact with things that may bear contamination. You may initially be surprised at how few spores light on your syringe from application to application.
Oh I know you are struggling with contamination sources and so you should be extra meticulous but try to learn this "second sight".
It will spare you from unproductive procedures.
Drop That Sound
Well-Known Member
Seeing that syringe filling station setup makes me wonder if you really need to use them at all, other that the initial purchase of a culture. Could you maybe just rig up a whole automated system using multiple different peristaltic pumps, and transfer everything through tubing and ports instead of doing injections?
I wonder if you could turbo charge the LCs even more by making some kind of bio reactor hydro setup, like they do with tissue culture explants to get even more constant DO for faster multiplication.. Maybe even put a small titanium cell with a little bit of current to produce some HHO gas through electrolysis. I've been reading lately about the benefits of hydrogen gas therapies, and drinking infused water, etc. I wonder how the mycelium would react to the broth being infused with pure hydrogen and oxygen.. Or if the sugars would react and create toxic byproducts..
I wonder if you could turbo charge the LCs even more by making some kind of bio reactor hydro setup, like they do with tissue culture explants to get even more constant DO for faster multiplication.. Maybe even put a small titanium cell with a little bit of current to produce some HHO gas through electrolysis. I've been reading lately about the benefits of hydrogen gas therapies, and drinking infused water, etc. I wonder how the mycelium would react to the broth being infused with pure hydrogen and oxygen.. Or if the sugars would react and create toxic byproducts..