Any Tissue culture threads?

[Mumbeltypeg]

Looking at setting up some basic equipment to give TC a crack.. looking for those who have ventured down this path

 

[GreenGenez421]

Looking at setting up some basic equipment to give TC a crack.. looking for those who have ventured down this path

I've been cultivating recreational, medicinal and gourmet mushrooms for years. The equipment needed for tissue culture is the same as mycology. Keeping things low key and basic you'll need a pressure cooker and a still air glove box, eventually upgrade to a laminar flowhood vertical is what i have, but a horizontal will work the same. I love my induction sterilizer but an alcohol lamp will work. Stainless steel tools are best but you can get away with disposable. Glass petri plates can be reused and sterilized, plastic are junk and most times not sterilized regardless of what they say. I'm not sure about the matrix you'll be needing but I'm pretty sure agar is what you will be using, depending on the development phase your targeting, hormones are needed to be added to the agar. And finally... glass jars to accommodate for vertical growth when they outgrow the petri plates.

This is an area I've been interested in for years, just never got around to it. I've got my hands full with everything I'm doing now. Good luck, your about to be sucked down a rabbit hole from which you'll never return. I absolutely love mycology, it's so rewarding, and I may have went overboard building a clean room and the hepa filtration, but cleanliness is key and sterility is king. You'll come to find all your "weakest links" that add to failures, 99% of the time.... it's sterility and cleanliness.

 

[Mumbeltypeg]

I've been cultivating recreational, medicinal and gourmet mushrooms for years. The equipment needed for tissue culture is the same as mycology. Keeping things low key and basic you'll need a pressure cooker and a still air glove box, eventually upgrade to a laminar flowhood vertical is what i have, but a horizontal will work the same. I love my induction sterilizer but an alcohol lamp will work. Stainless steel tools are best but you can get away with disposable. Glass petri plates can be reused and sterilized, plastic are junk and most times not sterilized regardless of what they say. I'm not sure about the matrix you'll be needing but I'm pretty sure agar is what you will be using, depending on the development phase your targeting, hormones are needed to be added to the agar. And finally... glass jars to accommodate for vertical growth when they outgrow the petri plates.

This is an area I've been interested in for years, just never got around to it. I've got my hands full with everything I'm doing now. Good luck, your about to be sucked down a rabbit hole from which you'll never return. I absolutely love mycology, it's so rewarding, and I may have went overboard building a clean room and the hepa filtration, but cleanliness is key and sterility is king. You'll come to find all your "weakest links" that add to failures, 99% of the time.... it's sterility and cleanliness.

Thanks bud, I’m looking at buying a lamina flow hood, steriliser and magnetic stirrer and some other bits from a mushroom farmer nearby in a few weeks.. sounds like there is a fair bit of crossover between the two, I’ll see what else I can pick up off him too then.

thanks again.

 

[GreenGenez421]

Thanks bud, I’m looking at buying a lamina flow hood, steriliser and magnetic stirrer and some other bits from a mushroom farmer nearby in a few weeks.. sounds like there is a fair bit of crossover between the two, I’ll see what else I can pick up off him too then.

thanks again.

Anytime! And, if you ever have any questions just drop me a line, I'd be happy to help wherever I can. Sounds like your going to be off to the races getting gear from a prior farmer. Excellent source!!!!!

 

[Drop That Sound]

 

[Drop That Sound]

Came across these LocknLock brand 2-way food storage containers, that come in a few different sizes. They're on sale on amazon, so I might just have to try using them to make some DIY manual plant TC immersion reactors.

Pretty sure they are 100% made from pp5 plastic, just like the mini twist top containers I use for no pour agar dishes for mycology purposes.

Not sure what material the o-ring gaskets are yet, but they are leak, microwave & dishwasher proof so hopefully hold up to high heat.

Should be able able to sterilize them right along with the liquid TC media in a pressure cooker no prob. Skip having to pour in fron of a hood or in a SAB.

Drill a hole on the side and slap on a filter patch, and looks like it would make a decent immersion "bio-coupler" reactor clone, for the multiplication stages of the ex-plants. Wrap around the lid area with parafilm for extra protection and seal.

You toss & turn them a few times a day in the reactor to submerge the plant for so many minutes, almost like flood and drain hydro but in vitro, so way better growth rates compared to just using the gelled media..

I was thinking about just RTV siliconing 2 regular twist top plastic food containers lids like the original biocoupler prototypes, but those locknlocks are already made with a one piece lid. Just drill a bunch of holes through it to make the screen\mesh between the 2 vessels..