Dude, i'm not inquiring as to motive, I am truly asking what you specifically plan to do with this. Like...are you looking to take a small amount of tissue and regenerate it? Or are you trying to like, keep one plant alive for a buttload of time, or what? no one has even addressed that in this thread, I'm still in the dark about what people are even tryin to do in the first place!
Keep your Mothers in a Jar!
- Thread starter xochipili
- Start date
Dude, i'm not inquiring as to motive, I am truly asking what you specifically plan to do with this. Like...are you looking to take a small amount of tissue and regenerate it? Or are you trying to like, keep one plant alive for a buttload of time, or what? no one has even addressed that in this thread, I'm still in the dark about what people are even tryin to do in the first place!
slackjack
Well-Known Member
Out of 10 fem seeds one plant actually has the pheno your looking for, you can use TC to reproduce that one plant for future grows, from there you can kill the mom, and continually take one leaf off your heaviest producing plant. If it takes 8 weeks to regen, then take one leaf at 6 weeks flower, that way, after this grow and one inbetween, you have healthy two week vegged plants ready for flower. By having all the same genetics, you can still isolate a single branch with the characteristics you want, and refine refine refine. If this is at all practical for home use, i promise it will take over connesuer buds.
cackpircings
Well-Known Member
I see sorry about that. My plan is to try it out with my plants I currently have I am going to try all kinds of different styles that I can come up with. I am also going to try to take some bomb bud that I purchase and try to see if by the luck of all things can get a baby out of it. If that be the case than I am going to not ever buy another seed if I like a bud I will just tissue culture it. Its more of a personal thing than any. I didnt even know this existed till I was reading this thread. SO now I am excited and it has been shipped. Once it comes I will do a new thread about it and keep you all in the know.
I see sorry about that. My plan is to try it out with my plants I currently have I am going to try all kinds of different styles that I can come up with. I am also going to try to take some bomb bud that I purchase and try to see if by the luck of all things can get a baby out of it. If that be the case than I am going to not ever buy another seed if I like a bud I will just tissue culture it. Its more of a personal thing than any. I didnt even know this existed till I was reading this thread. SO now I am excited and it has been shipped. Once it comes I will do a new thread about it and keep you all in the know.
Ah..ok, i'm sort of getting ya now.
Well, a few things to know. You need living tissue to culture from.
the younger the tissue, the better
the close to an apical point the better, a lot of tissue will not regenerate to new plants
regeneration frequency is very, very low. Expect 1 out of 10 'explants' to actually regenerate under perfect conditions.
your desire to take a bud you like and reproduce that plant...just, not gonna happen dude. I wish you the best of luck trying, but you need living tissue to make this happen.
Find a way to keep things sterile, or else you'll be wasting a lot of time and money. Good luck mang!
cackpircings
Well-Known Member
Thanks for the info bro, Im guessing you have exp in this area. I figured the bud wouldnt work, but I will defiantly still do a post up on how this all works just to give the option to the people.
It's my career actually, so yeah. Best of luck man, if you have any questions as you get going dont hesitate to message me, hopefully I can save you some time and money. Although, I know how fun it is trying crazy stuff, even with a low chance of success...so, have fun!
cackpircings
Well-Known Member
I will for sure I should get the package monday.
Sorry man, meant the apical meristem, i.e, actively growing tissue. Also, typically what is done is that very VERY immature tissue is used, like...7 or 8 day old sprouts, they are the best. Old tissue usually isnt because it exhibits very poor totipotency. Even if technically it can be, that might just mean that one out of a thousand will regenerate.
Veg DEFINITELY better than flower. The earlier the better, but thats sort of besides the point, we're looking to isolate the genes of a really good mother...so, someone might need to try with a plant well into flower. I've not put herb on agar before, so I really cant say. I've worked with Tomato, corn, soy and cotton...and what i've said holds true for all of them.
The experience you really need is aseptic technique. you need to be able to keep things STERILE!
and think about this guys. You want to take a piece of tissue from a plant growing in your closet, with all the normal bacteria and fungus that are on everything everywhere, and put that on sterile medium? you'll very quickly get fungal and bacterial colonies growing over everything, and it will be ruined. You can TRY and surface sterilize the tissue piece with .5 clorox .5 sterile water (and some TWEEN if you can find it), but that is a harsh treatment for tissue...and will likely damage the tissue too much. SO, when this is done the plant is grown sterily from seed, then the section we want to take is cut off, transformed, and regenerated into a whole new plant (containing the genes we want it to have...but this is of course for plant transformation, not just normal cloning) I dont really know how you'd get around the sterile needs of all this, so...some brainstorming will be required.
anyways, i'm rambling...maybe i'll start a real thread about this with pictures n crap instead. Time for a beer.
Titan4jah
Well-Known Member
time fer a j.
xochipili
Active Member
Yeah in the article it said to wash the plant in alchahol and then rinse it in a bleach solution, I thought this would be pretty harsh too, of course I have no doubt the manufacturer of this will spin it so it looks easy and foolproof , but hey it cant be any harder than getting mushrooms to grow, its basically the same techniques!
diemdepyro
Well-Known Member
Tissue culture is cool I have the time. Just can't see spending 250$.
I am the 5$ cheap pot mix method man.
I am the 5$ cheap pot mix method man.