I asked Tracy over @ BMO A few questions
(do i add water when the bottle gets a little gone ?and when i mix up 5 gal at a time and dont use it all how long do they live the)
Her responce
(You are welcome. Thank You for your business. Yes leaving the lid loose on the bottle so it can out gas and draw in fresh oxygen is a must. Stored in a cool, dark place the microorganisms inside will basically hibernate.
Their is no need to add water, but you can, as long as it is unchlorinated. If you pour out an ounce of SPT, then only put back in 1/4 ounce to prevent thinning down the mixture too much.
If you mix up 5 gallons of SPT solution, it needs to be applied within 24 hours for best results. After that the mix will begin to turn anaerobic. However, if you provide a constant air source (oxygen) from a small aquarium pump, and add one teaspoon of unsulphured molasses or sugar per gallon of solution, per week, you can keep the microorganisms in your bucket alive for up to a month. At a month, the number of microorganisms will be so great, they will literally starve and die off, unless you keep adding an increasing volume of food for them.
It is really best to mix up only as much as you need, as you go. This way the ratios of bacteria to fungi are at a premium and do the most good. If bubbled for long periods of time, like more that 15 days, these ratios can shift and slightly reduce the overall effectiveness of the product.
If we can answer any other questions for you, just send us a message. Thank You again.)
Just so you all know