Highest THC Content?

[FuegoDeBomba]

I never called anyone names, get over yourself.


That is my whole argument and nothing more!

"If you want to go on thinking cannabis is capable of structurally being 25% THC by volume then go ahead but it just shows your ignorance." along with your statement about me in this same quote.... You appear to not realize that your implications lead others to see you as the person you see me to be... "I never called anyone names, get over yourself." Guess C. Young is right about how we project on to others the negatives we dislike in ourselves.

You both have very valid intel on the subject matter. I was trying to show you how your both kind of at the same point. % of sample, though not measured in weight is still % of sample. GC actually combines compounds into THC, where as HPLC test separate even if they decide to combine later. Science is not 100%, Just like with any subject there are surveys and test to prove both sides.

Measure mass of liquids prior to mixing. Add these masses together
Mix the two liquids, let the reaction come to completion.
Measure the mass of products of reaction remaining in the container.
Subtract the final mass from the pre-reaction total mass. <----------- Complete this step to solve this issue, but it's not really needed for the answer to a collectives test question.

Find you common ground here and get back to the subject at hand.
You guys have good reputations here, so please use your intel to answer the purpose of the original question.

Again throw out the taste, look, and smell and focus on the high strength and stamina for the question of which strain was the best for you...?
For me, that I can remember there was this strain going around in the 90's in CA that was the truest sent of a skunks ass!!! The strength was so debilitating to the point where even though it was $65 for 3.0 I still would go back and get more just to show off to others. Names just weren't as important as they now. I want to guess it to be Skunk1 or Super Skunk and don't know if it was outdoor or indoor.
In the 00's so far... NL's Whites, TW's, Diesels, and OG's crosses are good beginnings, but some crosses dilute it more than improve it IMO.
GDP has been a solid staple for a long period of time and Humbolt growers products have never let me down! but like a few other great strains has no cups. (I don't think...)

My main grow till I find a suitable replacement you can't get anywhere because we only sell the flower and none of use the collectives because we get better value without them. We set friends up with clones, but seeds are fickle and and get you a bad rep. + I suck at sales... I'm a grower. I stick with this main and try to get snaps from other locals who have great non commercial strains, or try to go local recently with Cali Connect. Only them or Reserva Privada so far. Great stuff, but... IDK... Like I said before if you want to be great, put in the time and gain the experience on your own. THERE ARE NO MAGIC BEANS! Seriously, you can get that Y Griega or others and your end result may not be what you thought it would be...

Sorry if I came off like I was fully disrespecting you. I was just trying to make light of a discussion that's off subject and out of hand. I locked you in as a contact like a few of the others, if that says anything. I like the different fact checks and opinions.

 

[stonedmetalhead1]

"If you want to go on thinking cannabis is capable of structurally being 25% THC by volume then go ahead but it just shows your ignorance." along with your statement about me in this same quote.... You appear to not realize that your implications lead others to see you as the person you see me to be... "I never called anyone names, get over yourself." Guess C. Young is right about how we project on to others the negatives we dislike in ourselves.

LOL, that's far from calling someone names and then you turn around and do the same thing in your comment except you were being passive aggressive where as I was just being honest. "Guess C. Young is right about how we project on to others the negatives we dislike in ourselves. ", stop being a hypocrite.

 

[socalkushgenetics]

la confidential is a strain thats out now that has been blowin up. super potent. not sure on seeds

 

[TrichomeTrent]

la.con blew up a while ago hehe. the seeds are from dna i believe, although you can get the best phenos in collectives now instead of searching for your own

Anyways after reading some of this thread.... people need to RELAX haha. The cannabinoid ratios vary from plant to plant so saying 'x' strain is 'y.yy%' is just useless. Other than that i guess just keep debating, makes for a fun read

 

[Dmitri]

Potency is something to consider, especialy when growing/breeding for medical purposes. Even in that case, I don't think potency should be the ONLY concern. There are over 40 different cannabinoids....and only a few get you high. Yet, many of them do other things...like react with THC to make the high "up" (sativa's) or "down" (indicas) While other's react to make the high last longer...or make the person take longer to feel the enthogenic effect (creeper weed). Then theres terpinoids...science has discovered that they may help FIGHT OFF AND PREVENT SEVERAL TYPES OF CANCER! And the DEA and FDA claim cannabis has no medicinal value?

The FDA & DEA work for the Corporatocracy, which works for the banks, who work for the central banks, who work for the money masters. The Government is only one step or block above us (the people). Pharmaceutical corporations don't want cures or real treatments, to threaten their profits. Hemp Oil, DCA, Proper Nutrition (Gerson Therapy), Laitrile B-17, Dr. Royal Rife, Intravenous Vitamin C, etc. ARE ALL, NO NO's & a threat to "Public Safety".

 

[Cannalytics]

For those wondering about the general process of cannabis testing, here is an early peak at how we do it here in Michigan.

[video=vimeo;32336031]http://vimeo.com/32336031[/video]

 

[5ourdiesel]

For those wondering about the general process of cannabis testing, here is an early peak at how we do it here in Michigan.

[video=vimeo;32336031]http://vimeo.com/32336031[/video]

This video is cool and all if you have no idea how to load a gc, but there is nothing unique here about the process... though i am curious as to what internal standard you used in this video. every gc analysis on bud is done exactly this same way, though some choose whether or not to weigh out their samples.

one challenge is learning how to quantify the data from the gc but the single most difficult determinate in gc is selecting an appropriate internal standard and selecting an appropriate column whose stationary phase polarity must match that of the analyte.

here is a list of hp columns. columns in the first two groups would work best, IMO HP-1 column should separate cannabinoids pretty well based on their relative boiling points but hp-5 might also be possible.
http://www.chem.agilent.com/CAG/cabu/PropPhase1.htm

 

[FuegoDeBomba]

This video is cool and all if you have no idea how to load a gc, but there is nothing unique here about the process... though i am curious as to what internal standard you used in this video. every gc analysis on bud is done exactly this same way, though some choose whether or not to weigh out their samples.

one challenge is learning how to quantify the data from the gc but the single most difficult determinate in gc is selecting an appropriate internal standard and selecting an appropriate column whose stationary phase polarity must match that of the analyte.

here is a list of hp columns. columns in the first two groups would work best, IMO HP-1 column should separate cannabinoids pretty well based on their relative boiling points but hp-5 might also be possible.
http://www.chem.agilent.com/CAG/cabu/PropPhase1.htm

Agreed! Is there a continuation to this video where they show some data?

Though I still don't think the %'s carry any weight of importance on the original question. Trying to calculate an experience is difficult and subjective... How do we tie the experience to the data with any kind of consistency to validate a specific strain?

 

[Cannalytics]

We use HPLC, not GC, which allows us to quantify the acidic cannabinoids in addition to their decarboxylated counterparts. This is especially important for analyzing products intended for oral consumption since they will not be decarboxylated again before ingested by medical cannabis patients.

An internal standard is one that is mixed with the sample of interest during injection to correct for any variability in injection volume. Internal standards are molecules with similar chemical properties to those in the sample that would however not be contained in the sample. We do not use these because our auto-sampler is extremely accurate & precise at injecting the same volume for each sample tested. Internal standards would be more important for those manually injecting each sample since for these types of systems, this step can be a major source of variability. We do however use external standards. These are ordered from chemical suppliers and come with certificates of analysis to demonstrate their purity. We run 3 of these at the beginning of each batch of tests and another after every 5 samples.

For an example of how the math is performed, see this example on our site: http://www.micannalytics.com/results/sample.php?sampleid=111280&dispensary=55

Below the calculated results, we have a section called "Primary Data." This includes all of the measurements taken by us to make the calculation. The formula for calculating percentage potency by weight is as follows:

% (mg/mg) = (area of sample / area of standard) * (concentration of standard) * (total volume of extraction solvent) / (mass of sample)

% CBD = (386.5 / 9130.1) * (0.25mg/mL) * (10mL * 21) / (437.4 mg) = 0.5%

Please let us know if you have any additional questions. Cannalytics has been committed to making our results as transparent as possible which is why we have made the primary data available for every result available on our site.

 

[stonedmetalhead1]

We use HPLC, not GC, which allows us to quantify the acidic cannabinoids in addition to their decarboxylated counterparts. This is especially important for analyzing products intended for oral consumption since they will not be decarboxylated again before ingested by medical cannabis patients.

An internal standard is one that is mixed with the sample of interest during injection to correct for any variability in injection volume. Internal standards are molecules with similar chemical properties to those in the sample that would however not be contained in the sample. We do not use these because our auto-sampler is extremely accurate & precise at injecting the same volume for each sample tested. Internal standards would be more important for those manually injecting each sample since for these types of systems, this step can be a major source of variability. We do however use external standards. These are ordered from chemical suppliers and come with certificates of analysis to demonstrate their purity. We run 3 of these at the beginning of each batch of tests and another after every 5 samples.

For an example of how the math is performed, see this example on our site: http://www.micannalytics.com/results/sample.php?sampleid=111280&dispensary=55

Below the calculated results, we have a section called "Primary Data." This includes all of the measurements taken by us to make the calculation. The formula for calculating percentage potency by weight is as follows:

% (mg/mg) = (area of sample / area of standard) * (concentration of standard) * (total volume of extraction solvent) / (mass of sample)

% CBD = (386.5 / 9130.1) * (0.25mg/mL) * (10mL * 21) / (437.4 mg) = 0.5%

Please let us know if you have any additional questions. Cannalytics has been committed to making our results as transparent as possible which is why we have made the primary data available for every result available on our site.

Do you have a pure standard for every compound that creates a spike in the chromatogram? If so could you please disclose what they are. Also how many cannabinoids are you testing for?

 

[indagroove]

Puffing this now:

 

[Cannalytics]

Do you have a pure standard for every compound that creates a spike in the chromatogram? If so could you please disclose what they are. Also how many cannabinoids are you testing for?

For each sample posted to our site, the "Primary Data" section includes links to download the chromatograms for the individual sample, as well as the combined chromatograms of all of the reference standards run that day. To see a chromatogram of our reference standards, see http://www.micannalytics.com/chromatograms/standards/2011-11-29.pdf

The order of elution of the compounds (from left to right, first to last) is CBD, CBN, THC, THCA. We also recently received standards for CBC & CBG which we should be testing for soon.

Please let us know if you have any additional questions.

 

[FuegoDeBomba]

Puffing this now:

Is this your personal test or a collectives? Do you know how much SC Labs charges for a test? Sour OG (along with K-Train, OG47, and maybe Purple Chem) is one I'm really interested in growing, but I wasn't to impressed with the Chem4xOG from CC even if it does demand top dollar at the collectives. CC's Sour OG won Michigan through a 3rd party grower though...

How does it Hit? Is the look and scent good too??

 

[stonedmetalhead1]

For each sample posted to our site, the "Primary Data" section includes links to download the chromatograms for the individual sample, as well as the combined chromatograms of all of the reference standards run that day. To see a chromatogram of our reference standards, see http://www.micannalytics.com/chromatograms/standards/2011-11-29.pdf

The order of elution of the compounds (from left to right, first to last) is CBD, CBN, THC, THCA. We also recently received standards for CBC & CBG which we should be testing for soon.

Please let us know if you have any additional questions.

So you're not claiming % weight compared to the whole sample? Because you don't have enough data to determine that based on your chromatogram.

 

[FuegoDeBomba]

For each sample posted to our site, the "Primary Data" section includes links to download the chromatograms for the individual sample, as well as the combined chromatograms of all of the reference standards run that day. To see a chromatogram of our reference standards, see http://www.micannalytics.com/chromatograms/standards/2011-11-29.pdf

The order of elution of the compounds (from left to right, first to last) is CBD, CBN, THC, THCA. We also recently received standards for CBC & CBG which we should be testing for soon.

Please let us know if you have any additional questions.

Cannalytics- don't you guys test for THCV & I thought I saw THCB as well? Is there any chance you maybe able to break down each compound tested to it's effect for us??
I've seen a video on THCV saying it is most responsible for the psychedelic effect and CBN is most responsible for the sleepy effect... Do you have any data on, or different from this?

Here's another pretty well detailed video from SC LABS http://www.youtube.com/watch?v=r2QC9b90loM&feature=related It will probably stir the pot again since they weigh the entire sample

 

[indagroove]

Is this your personal test or a collectives? Do you know how much SC Labs charges for a test? Sour OG (along with K-Train, OG47, and maybe Purple Chem) is one I'm really interested in growing, but I wasn't to impressed with the Chem4xOG from CC even if it does demand top dollar at the collectives. CC's Sour OG won Michigan through a 3rd party grower though...

How does it Hit? Is the look and scent good too??

No, it's not my personal test, it's from my local collective but I did purchase an 1/8th of it from them. It smells great, burns and tastes good. It's a little on the airy side, but not fluffy really -- just more like Sour D than OG in terms of the bud formation. I've got a plant of Sour Buddah (SD x Hindu Kush) going at close to 7 weeks flower right now, and it looks and smells fairly similar. I can't say for the Sour OG, but my Sour Buddah is looking to be clost to a 10 week strain, which is a drawback for me. I like 8 week strains. I did clip a lower bud in the microwave last night though, and was very surprised about the potency as premature as it is.

 

[Cannalytics]

So you're not claiming % weight compared to the whole sample? Because you don't have enough data to determine that based on your chromatogram.

From the chromatogram of the sample extract alone, you're right, it is not possible to calculate the percentage by weight. However when combining this information with the chromatograms of the standards and the weight of the sample from which the extract is made, these calculations can be made according to the formula I previously posted.

 

[Cannalytics]

Cannalytics- don't you guys test for THCV & I thought I saw THCB as well? Is there any chance you maybe able to break down each compound tested to it's effect for us??
I've seen a video on THCV saying it is most responsible for the psychedelic effect and CBN is most responsible for the sleepy effect... Do you have any data on, or different from this?

Here's another pretty well detailed video from SC LABS http://www.youtube.com/watch?v=r2QC9b90loM&feature=related It will probably stir the pot again since they weigh the entire sample

I'm familiar with the SC Labs series of videos. They've partnered up with WeedMaps so they've got access to more resources than we do but we plan on producing similar ones targeted towards patients in Michigan.

Unfortunately, there are no commerical standards available for THCV and many of the other minor cannabinoids, however we have made requests with our suppliers to provide these compounds for us. The only other option is to custom purify these compounds in house however this would require the use of a mass spectrometer, LC, and fraction collector. We plan on doing this in the future but lack the mass spec at the moment.

 

[stonedmetalhead1]

From the chromatogram of the sample extract alone, you're right, it is not possible to calculate the percentage by weight. However when combining this information with the chromatograms of the standards and the weight of the sample from which the extract is made, these calculations can be made according to the formula I previously posted.

How many tests are you running on each sample?

 

[Cannalytics]

How many tests are you running on each sample?

Before answering this question, I think it is important to distinguish between technical & biological replicates. A technical replicate would be injecting the same extract into the machine more than once in order to determine that the machine is precise in making it's measurements. We do not perform technical replicates of each sample because the data collected from multiple injections of the standard solution demonstrates that there is very little variability from injection to injection. The daily relative standard deviation (% RSD) is available for each of our samples under the "Primary Data" section. As for biological replicates, this would be independently processing multiple instances of the same sample from start to finish.

Getting back to your original question, I assume that you're interested in the variability of biological replicates. For homogenous products, such as edibles, concentrates, and tinctures, we are able to analyze the same sample repeatedly and come back with tightly correlated results. For heterogenous products, such as the buds produced by a plant, there are a number of factors that can lead to variability, such as position within the canopy, the presence of mixed phenotypes, and the degree of curing/evenness of moisture concentration (wet buds test lower than dry buds). For these reasons, sampling is the biggest source of variability for producing our results. We encourage our clients to submit several small pieces taken from several buds within the batch in order to provide the most accurate results possible. Obviously, submitting the prized cola bud will result in an overly inflated result for the batch and submitting a small, under-developed, uncured bud will result in an under-inflated result for the batch. For these reasons, each set of results generated comes with the disclaimer that "these results pertain only to those items tested."