Marijuana Tissue Culture Success!

pharmacoping

pharmacoping

Active Member
I do this very well, and assemble kits for sale too /prepared solutions/pre filled sterile containers/proper nutrients, and even genetic specimens, for observation purposes. Some samples include Thyme, Rosemary, Tahoe OG Kush,PlushBerry,Kandy Kush, and hundreds of other non plant dna samples. The study of these samples will can prepare you for the art of tissue cultivation/cloning. From a single spec thousands of master programmed clones can be taken monthly ! Imagine no more mother plants or clones using your plant spaces? How important is that to you? These are stored/divided as masses of tissue or unusable roots. Only in the last few days do they become a "plant". You can easily have roses with thc, or mj without leaves, or even glow in the dark, really, I've seen it ! It's not the future, it's now ! Patents are already being granted for different mj types grown this way. suicide genes,inability to clone/seed..are some of the programing in these super corpopharma plants. Instructions are available online, but the right mixes are almost impossible to find and invaluable to growing your own rare herbs successfully, and storing their dna forever. With these supplies you'll learn how to master this fast because the work is done !! Plan on spending a couple hundred bucks or less, depending on the equipment you have at home...jars/mixer/pressure cooker,etc.

I'll answer some q's here, but private message me for instructions for above.
pics coming soon, soon as I figure that thing out !

peace​
 
cowboylogic

cowboylogic

Well-Known Member
I dont doubt the concept or science. But a rose w/THC, thats a pretty bold claim for the home lab. I am subbed, would really like to see more details on your techniques...
 
shadowdarker

shadowdarker

Well-Known Member
sounds good would like to know more!! sounds similar to taking mushroom spores and not far off the kit you need for growing mushrooms am i on the right lines??
 
ddimebag

ddimebag

Active Member
Im not really an expert on the subject, but cannabis tissue culture (cloning from plant material) and making transgenic plants (roses with THC, glowing cannabis, presumably with a GF1 gene) are very different things...as far as I know, genetic modification of anything is beyond the abilities of anyone without a degree in genetics or molecular biology...but if I am wrong, I would love to see your technique.
 
Guile

Guile

Active Member
Dude, please.... don't go selling bullshit here.... please.... this place is fucked up enough as it is... seriously you would not believable the shit we already encounter... You have no idea what we put into trying to help people see reality....

I seriously hate shitting on anyone or their beliefs but come on... really...

If you have something so amazing, prove it by shearing it for free and allowing it to be proven.... otherwise stop spamming my chosen spot so that we can have clear minds and understandings of shit...
 
pharmacoping

pharmacoping

Active Member
Yep, you are on the correct line, very very similar to mycellium expression.
I'm not trying to sell anything, or spread rumors or spam. The mj community
is full of misinformation, unfortunately.

take a few hours and google, you'll find pictures and descriptions of all of this, in big business.
I dont care about roses with thc, or mj with tendrils, or even glow in the dark, all evil to me.
I really dont want to play with magic I do not understand. However, this is very basic science
and can bring a growers garden to the highest level in production, quality,uniformity, solving very serious legal issues
involved in the production of this plant. This is not for the closet grower, or a person who is not familiar with
sterile technique, which can be learned in a day and very successful. This area of plant science is has been
brought to the cutting edge with a cloud of magic and voodoo. I have mastered these techniques over many years.
Before you could dot com for proper supplies I was using gelatin and crushed vitamins, with naturally derived plant hormones,
with some successes. I failed a million times in the beginning, but continued with my passions. I've arrived and
only wish to share this with the mj community now. Pull up your big boy pants you all, its gonna get techy. I do not lie,nor
will I debate the existence of this technology here. do your own homework, come on back, and lets get culturing.


breeding, dna manipulation, insertion, etc is performed with the introduction of the desired dna strand
via bacterium, by the way, and is being performed by many tc dudes around the world, in their mini labs.
 
Guile

Guile

Active Member
pharmacoping said:
Yep, you are on the correct line, very very similar to mycellium expression.
I'm not trying to sell anything, or spread rumors or spam. The mj community
is full of misinformation, unfortunately.

take a few hours and google, you'll find pictures and descriptions of all of this, in big business.
I dont care about roses with thc, or mj with tendrils, or even glow in the dark, all evil to me.
I really dont want to play with magic I do not understand. However, this is very basic science
and can bring a growers garden to the highest level in production, quality,uniformity, solving very serious legal issues
involved in the production of this plant. This is not for the closet grower, or a person who is not familiar with
sterile technique, which can be learned in a day and very successful. This area of plant science is has been
brought to the cutting edge with a cloud of magic and voodoo. I have mastered these techniques over many years.
Before you could dot com for proper supplies I was using gelatin and crushed vitamins, with naturally derived plant hormones,
with some successes. I failed a million times in the beginning, but continued with my passions. I've arrived and
only wish to share this with the mj community now. Pull up your big boy pants you all, its gonna get techy. I do not lie,nor
will I debate the existence of this technology here. do your own homework, come on back, and lets get culturing.


breeding, dna manipulation, insertion, etc is performed with the introduction of the desired dna strand
via bacterium, by the way, and is being performed by many tc dudes around the world, in their mini labs.
Click to expand...
Alright, I call you on it....

I have placed this quandary before so I will do it again... Can you use the node section of your particularly Favorited (freshly harvested lady) to create an army of clones using little more than a cleaning agent and gel medium containing hormones and nutrients? if not what circumstances are required for success? Time to step up and be judged, what is your method?
 
Z

Zedgreen

Member
I've looked into tissue culture and yes it can be done at home not to scale you talk about.
But i have seen no one do it with MJ and show start to finish proof that it works and the steps required. Every plant is different the same medium to produce tissues form rose may not be the mix you need for MJ.
From what I've read Mj is a woody plant and the best way to do tissue cultures is using stems and have it go up hundreds of shoots.
 
pharmacoping

pharmacoping

Active Member
...of course I can operate a camera, but I just received a new one and didnt even put batteries in it yet. The one I have does not have macro, so I upgraded, geesh.
open your minds fellow stoners, just because you cannot imagine something, does not mean it can't exist, very obtuse. But for the smarty pants tokers, here you go:
bacterium and virus' are used (along with antibiotics) to insert foreign dna into plasmid dna, then inserted into a host bacterial cell, and if the host cell is compatible, this often will happen naturally, otherwise electricity is used to join the group.when a cells walls are "perforated, with the use of chemicals/electricity/enzymes,it allows the fragmented/foreign/programmed/radioactive etc. dna to incorporate into the host cell chromosomes=homologous recombination.
I did not say that I make any of these things happen, only that it could be done. I culture root masses, divide them, and even make some express themselves vertically and laterally, when needed, weekly usually. I do not frankenstein, or breed, or glow in the dark, or make roses with thc, as I've already stated, so it will be very difficult for me to photograph procedures and results, from these technologically advanced procedures, that I have not performed.
I did say it is being done. Google some books, you'll arrive safely, and all your fears will subside, I promise.
Some of these procedures can and are being performed in mini home labs,identical to mine even, and big pharma ones too. I don't want to debate the existence of this technology, but would like to speak with world wide private individuals who have "crossed over". I have met hundreds online, and a dozen or so in person, with varying degrees of awesome successes. I dont mind sharing ideas, but every bit of this info is available online, amazon, ebay, google, so c'mon, go read, I'll see you in a bit.

Most stoners wont do this, many cannot, and many more can't accept that it exists. I'm not here to convince you either way. I did this with crushed vitamins and supermarket gelatin, more than 20 years ago kids, accept it, and lets move forward. Not sure the picture efforts are worth it, but you can private me for some, if serious, peaceful, respectful attitudes are displayed. I cannot believe the attack on new technology from my brothers. But now I understand why there is so much growing misinformation in this community.

If you currently tissue culture any explants at all , please chime in.

You are correct, specific mixes for specific plant genus. Trial and error found my mix for marijuana. Corn and tomatoes were easier to figure out, and now, all but the mj mixes can be found online, saving thousands of hours and years of research for us. I cant walk anyone through this process in this forum, but after you read ahard cover book or two, labeled Tissue Cloning, I will try to answer questions. Beware, I'm not a scientist, but a stoner. I dont have a masters degree, yet, and have been growing exotic plants of many species, hydroponically, since the mid 70's. Tissue Culture is the tip of hydro technology, growing without photosynthesis, and in a sterile mini greenhouse

peace, already:leaf:
 
pharmacoping

pharmacoping

Active Member
genetic manipulation happens on a daily basis in each container I culture. These sterile bits of dna are programmed with varying amounts of their own hormones that control many expressions. Genetic manipulation/modification is and does take place everytime you seal a jar here. Sometimes with unwanted results. It even happens in the "knots" of your stalks, often, and protective enzymes work hard to halt the mutant genes from multiplying. I have used this particular infected tissue group and seen very strange things. OK, maybe not "easily" done, but far out expressions are being programmed as we speak at home labs, like glow in the dark, for sure.
 
pharmacoping

pharmacoping

Active Member
Guile,
In short, Yes you can my friend, and within a few days you will understand the magic. A node is only one part of this plant that can be used. The hardest one to root is a mj leaf bit, but I've seen it done. The newest growth is the best, not the top cola, but new lateral tips. Shoots from flower meristems, seeds, root bits, stalk, etc are successful
they all express differently sometimes. The shoot tips are typically virus free and therefore are preferred, by some of us. I like seeds sometimes also, aseptic germination of one seed can produce multiple thousands of shoot tips within a month, literally, and then days from that culture, exponentially. Unfortunately a seed culture may produce a thousand identical but unacceptable genetics, wasting your tc time. Its best to start with a clean healthy femaile, not feminized, plant.
I have stepped up, and am judged, and you have my exact methods down my man, in alot less words than me. sterile, sterile, more sterile, and then sterilize. I use a large walk in zip up grow tent, sealed from the outside, with positive hepa filtered air flowing through a small "flow hood", a tupperware container on its side to keep molds and mites out while transferring. otherwise the tent is sprayed with lysol at everyentry and exit inside an out. tools are alcohol flamed, everything, water too, is sterilized in a pressure cooker for 25minutes or so. I can mix agar,hormones, water in sensitive and delicate measurements , in my sleep. all is sterile when used. just like shrooms, and canning, sorta.
This came to me to beat the plant count, putting 25-30 more plants in play, with no bs in a cloning tray, taking up the count while rooting. These take up count only for a few days, and precisely when i order them to, then they harden off, and are put in veg at about 1.5 in tall for 45 days, 24 inches, strain depending, packed with branches,budding sights, like a bonzai, flowering in 60 days exactly, at about 36 inches tall, harvested weekly, year round, to stay legal, with impeccable timing, and a perpetual garden. thats me, no bull, not a kid
 
Guile

Guile

Active Member
pharmacoping said:
...of course I can operate a camera, but I just received a new one and didnt even put batteries in it yet. The one I have does not have macro, so I upgraded, geesh.
open your minds fellow stoners, just because you cannot imagine something, does not mean it can't exist, very obtuse. But for the smarty pants tokers, here you go:
bacterium and virus' are used (along with antibiotics) to insert foreign dna into plasmid dna, then inserted into a host bacterial cell, and if the host cell is compatible, this often will happen naturally, otherwise electricity is used to join the group.when a cells walls are "perforated, with the use of chemicals/electricity/enzymes,it allows the fragmented/foreign/programmed/radioactive etc. dna to incorporate into the host cell chromosomes=homologous recombination.
I did not say that I make any of these things happen, only that it could be done. I culture root masses, divide them, and even make some express themselves vertically and laterally, when needed, weekly usually. I do not frankenstein, or breed, or glow in the dark, or make roses with thc, as I've already stated, so it will be very difficult for me to photograph procedures and results, from these technologically advanced procedures, that I have not performed.
I did say it is being done. Google some books, you'll arrive safely, and all your fears will subside, I promise.
Some of these procedures can and are being performed in mini home labs,identical to mine even, and big pharma ones too. I don't want to debate the existence of this technology, but would like to speak with world wide private individuals who have "crossed over". I have met hundreds online, and a dozen or so in person, with varying degrees of awesome successes. I dont mind sharing ideas, but every bit of this info is available online, amazon, ebay, google, so c'mon, go read, I'll see you in a bit.

Most stoners wont do this, many cannot, and many more can't accept that it exists. I'm not here to convince you either way. I did this with crushed vitamins and supermarket gelatin, more than 20 years ago kids, accept it, and lets move forward. Not sure the picture efforts are worth it, but you can private me for some, if serious, peaceful, respectful attitudes are displayed. I cannot believe the attack on new technology from my brothers. But now I understand why there is so much growing misinformation in this community.

If you currently tissue culture any explants at all , please chime in.

You are correct, specific mixes for specific plant genus. Trial and error found my mix for marijuana. Corn and tomatoes were easier to figure out, and now, all but the mj mixes can be found online, saving thousands of hours and years of research for us. I cant walk anyone through this process in this forum, but after you read ahard cover book or two, labeled Tissue Cloning, I will try to answer questions. Beware, I'm not a scientist, but a stoner. I dont have a masters degree, yet, and have been growing exotic plants of many species, hydroponically, since the mid 70's. Tissue Culture is the tip of hydro technology, growing without photosynthesis, and in a sterile mini greenhouse

peace, already:leaf:
Click to expand...
I have been looking into hormones lately, if you look hard enough you will find threads here titled in ways that suggest it... In all my efforts I have found many papers on tissue culture but only one guy here that has first hand experience to offer... Even he dances around answering some of my questions and its my impression that things are just not as simple as I would like them to be... That having been said I'm not a complete idiot... Tissue culture does exist but it is focused on certain tissues to start with... beyond that it seems to be a bit involved to get other tissues/cells to develop once you've started... for instants you can grow a plant without roots from the cells gathered from a growing tip but at some point you have to develop roots through a separate hormonal manipulation its at very least a 2 part system... I know tisue culture can also start at the roots but at some point you will have to work your way up (not sure how that works but I'll give it to you as you are clearly trailing at this point).

I believe that there is some truth to the essence of what you are saying.... I also think you should try it out, or at very least read more about it before you promote it... I believe with my current level of understanding I might be able to clone a plant from cellular material (though it would not include introducing any bacterium, infact it would take great pains to avoid it) I also believe it would be incredibly inefficient overall, at least on the scales most of us work on.

And then again perhaps I have things miss judged... I really don't know any better (not first hand anyway) I am willing to be wrong, though I would prefer to be proven that way (before I concede anyhow). I still think waiting a month for the propagation of new growing tips is a bit inefficient especially in the scope of my thought that they likely don't have roots...

Yes it seems the newest growth works best for cloning on a cellular level, its been explained to me that these young cells are more easily manipulated around by "hormone therapy" so to speak. you are essentially bossing around the cells to do what you want using hormones to guide the way... the thing is that most study's I ave read only utilize a couple hormones at a time at cirtian atages of development.. I've honestly never seen anything that goes from cell culture to harvest (not for pot anyway)

Please forgive my ignorance, I would appreciate you pointing me in the right direction to rectify this dilemma...
 
jcdws602

jcdws602

Well-Known Member
Usually when things sound too good to be true they are......this thread is like an infomercial....BUT WAIT,THERE'S MORE..!!
 
pharmacoping

pharmacoping

Active Member
wow, not sure what to say. pictures=proof? really? matter of fact I can no longer assemble kits. I've been approached, LLC'd webbed,blogged,amazoned,and ebay'd by a very professional gentleman in a nearby town. If you beg hard, I'll share the website when released. so there. not selling a thing.

Correct, I grow callus' of a few dozen strains, they divide and multiply in the same solution, indefinately, maybe. when needed I incise a piece of this callus and transfer into a fresh, newly "programmed petri dish, jar, deli container(sterile of course)" and cause it to immediately begin rooting, with no plant count numbers being used yet, then I have several strains in "rooting stasis", from a few days, to a few months. it can be divided and re cultured also, but thats prolly way to complicated for some here so I'll stick to straight forward. when ready, now heres the whole point in a rotational, year round,perpetual, legal garden running max #, the root masses are told to shoot up, and they do, with a broccolli head bundle of branching,nodes,and leaves, bonzai like. this takes a couple days, during which they use up a plant count number until they are done flowering-rather than maintaining plant counted mothers, and their clones while they root, these are super, pure plants ready to perform. three days after told to shoot up they go to harden,2 days later in veg for 45 days, couple feet, to flower. this happens weekly here in my garden, and has for a long time. This does not replace cloning, necessarily, for the everyday grower. A cropper needs this, and would recognize its value immediately, if trying to stay legal throughout their grow, and not having to buy or introduce clones to their garden. A perpetual weekly legal harvester needs this also, and could see the magic, if even with some 'splainin, which I will freely do. Imagine having 72 rootballs, with hundreds at the ready, to use a few days to sprout, while you clean your grow room for the next harvest. you cannot do this with a cloning program and be legal. 72, in MI is the law. If I seem to avoid things , I'm sorry, I am a stoner after all, who is under attack. pics ar coming soon, I promise

but please, if ya just figured out how to sex your plant, or why you shouldnt use molasses indoors, find another forum to vent in. There are mini horti labs nationwide, doing the same thing. they frown on sharing info with folks like you, or anyone else for that matter. Please have respect, and lets learn from eachother. enouh with the fruit and veggie toss here, this is real kiddies, I'm not a bulshitter, or spammer, or trying to sell dick. grow the fug up and learn sumthin already. some of us are very serious at what we do,have a calling even. We've been out of our moms basement for decades. Bubbling buckets probably still stored in the shed there, from 30 yrs ago !
 
shadowdarker

shadowdarker

Well-Known Member
im going to come along for the ride to see what happens on the thread. am very interested in what your saying only have a very basic understanding but i can keep up with what your talking about. i grow mushrooms with great success so this would be the next step.
 
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