readysetgro
Member
Read the whole thing its pretty informative, nutrients, recipes, etc, these guys rock.
Nope, but the lids should be the type that breathe a bit, or I use micron filter patches over a hole drilled in a babayfood jar lid. the only time you open the jar is to transfer your material, I even syringe inject through the micro proe filter down into the agar beneath the calli, to avoid a couple transfers.should these TC be burped for fresh air?
Cloning MJ tissue samples, well we all saw the rat with the human ear growing on his back, that would be really awesome if next u see a rat running around with a MJ plan growing off its back!!! Lol ok well it sounded funny when I said it to myself.
Read the whole thing its pretty informative, nutrients, recipes, etc, these guys rock.
good to see you !!hey pharm been a while just wanted to let you know my progress. i have pretty much given up on a DIY setting for my tissue culture setup and decided to save the cash to do a real set up in the future have a lot of materials collected(hepa filter/fan, jars pressure cooker alchhol burner, forceps, stainless steel table small t5 light set up tyvek filters, and more) but still have a way to go(still have to order my hormones and such). do you know any places to get glass petri dishs cheap? or do you use canning jars only?
long time no see too.
I've been fascinated with this concept before Jurassic Park. I so wanted to bring life to the dead !. I can do it, sort of, but not from several year old material, although that is certainly able to be done, but I need a half million dollars more to do it.Intresting thread, I have been following it from the beginning all morning. Are there any more picture updates to those that have been posting? I will be purchasing my materials to build a lab this wknd, & hopefully be proceeding to tc next week or so thanks to al the info ive found here! Never has it been made as plain for me. Anywho I plan to tc some big buddha blue cheese & some th seeds s.a.g.e. I also have some stalks from some local strawberry cough that a friend saved for me. Im most excited about these bc the stems are a year or so old & no longer green, so i cant wait to experiment on bringing them back to life Great thread, great info i will have plenty of ?s & lots of pics coming up! Thanks!!
If you're just storing/growing clones no need to harden. Not sure bout the gas, guessing its the beginning of a new life form though. My explants are smaller than a pencil eraser and widdled down to a thread. These will grow into undifferentiated callus very quickly, then I cut pieces off of this and root/chute them, then to the veg room, keeping the original culture(s) as mother plants to cut from.Im as femme as my clones----- (girl) Newho so i soaked in a bleach solution then peroxide cuz i had no alcohol & im just all for the experiment, & i took cuttings & put them in a homemade bubbler. Some of the stems changed by color & feeling, a few seemed hollow inside & didnt do anything. Ive taken 6 cultures from 3 stems that are under a 75 watt?? Im not sure the wattage but its a long tube fluor with some kinda grow bulb (from home depot). I see the calus & a little mound of "stuff" forming under the cutting, I see growth in size, no new leaves, & it looks like a gas may be in the jars?...Is this normal? Also I have them in these little 4oz ball canning jars, but it looks like theyll grow out of them in a few weeks, do i transplant them to new jars or how do i know when theyre ready to be hardened & put into a medium? Hope I didnt lose u lol
thats exactly what we're doing. any part of the plant, leafs, stalk, bud, anthers, stamen, pistil, root can be cloned, because the plant is totipotent, it has all the genetic make up necessary to produce. Of course you you could have a stick with a bud on it, but why? may as well grow out an auto flowering strain...which also can be cloned in vitro and kept like a mother plant.just a blazed question but would it be possible to clone a bud and grow it in a tissue culture?
so you can clone autos in vitro? how does that work? + rep btwthats exactly what we're doing. any part of the plant, leafs, stalk, bud, anthers, stamen, pistil, root can be cloned, because the plant is totipotent, it has all the genetic make up necessary to produce. Of course you you could have a stick with a bud on it, but why? may as well grow out an auto flowering strain...which also can be cloned in vitro and kept like a mother plant.
I get my (gerber only,lids) from the grocery store.umm where did you get your baby food jars?
and your saying i should just forgat about hormones and learn the old fashioned way still because MJ is totipotent?
yep, besides making a plants bioluminesce successfully, cloning/storing autoflowers has been my funnest experience. In vitro they'll slow vegetation down, to a near stop, divide, sprout, root normally, and I constantly use my santa maria auto mom in a test tube !so you can clone autos in vitro? how does that work? + rep btw