Halllppp!!!:(

[polyarcturus]

okay let me start by saying i could post pics, but i dotn think that wil help since what im seeing is white too.

3 jars of GT. i shook them one day. few days later white "specks" showed up. so im thinkin okay chunks of mycelium. now one of the jars has had rapid expansion of these specks, in addition too being covered in mycelium, a few of the speck on this one has turned grey.

one speck, has formed its mycelium, and it looks, less dense of a structure than the GT mycelium.

im assuming this is contaminated, but it is all white, i would also like to note that condensation, that was not there has built up on the jars too.

last but not least i have one of these contaminated jars, only the top inch or so is contaminated(has these specks), if i remove the contaminated layer is it possible to save it?

what should i do with the other 2 jars?

ill get some pics, and i will try to take good ones but it will be hard to tell.

 

[polyarcturus]

substrate, popcorn

 

[The New Jim Jones]

i wouldnt smoke any of that, you might have to throw it away. Do you think you could make hash with it?

 

[polyarcturus]

i wouldnt smoke any of that, you might have to throw it away. Do you think you could make hash with it?

its not weed goofy. its fucking mushrooms. yeah im gonna go make bubble with it roflmfao

 

[The New Jim Jones]

aahahahahahaahahahahahahah yeah i realized my mistake too late, i find myself doing that too often, ahahahahahahahahahah i sounded like a total idiot, im not going to delete it because its too funny. well im out of my element when it comes to shroomies, only eaten em a couple of times, and i never stored them, it was buy and then almost immediate use, sorry ahahahah

 

[polyarcturus]

aahahahahahaahahahahahahah yeah i realized my mistake too late, i find myself doing that too often, ahahahahahahahahahah i sounded like a total idiot, im not going to delete it because its too funny. well im out of my element when it comes to shroomies, only eaten em a couple of times, and i never stored them, it was buy and then almost immediate use, sorry ahahahah

its all good, made me laugh too.

 

[Jogro]

Condensation doesn't mean anything.

Psilocybe mycelium is white and fluffy and gives off a distinct fruity smell. If that's all you see, you're good.

Contamination is yellow, brown, black or grey. If you're not sure if a jar is contaminated, just wait. If it is, you'll know within a few days (the jar will usually start to smell bad, too).

There is no practical way to fix a jar once its contaminated. Throw it away, and start a new jar.

And here's a tip for you, I don't know if people are using this nowadays, or if its written anywhere, but years ago when I used to do this, I would use dirt cheap birdseed as a substrate, and it worked great. Kept the sunflower seeds in there too. They didn't really add anything, but they didn't hurt, either and it was too much work to pull them out.

 

[polyarcturus]

contamination came from when i tried to shake jars, must have got filter patch wet and sucked something thorgh. not the popcorn.

 

[NavySEALsVet]

Popcorn sounds good right about now

 

[polyarcturus]

first jar

second jar

 

[canndo]

Chuck them - you got a single contam spore in there, it grew a bit and then when you shook, you spread them all over. It happens. What are you using for filters on those jars?

 

[polyarcturus]

small holes (bout 1/4") backside slatheried in RTV and a square of tyvek carefully placed and pressed down.(take a little practice cause RTV will come through the tyvek, and you got to watch getting it on your fingers and spreading it all over the patch)

these 2 comtams happened after i shook them they where sterile up till then im sure, remember these jar sat and incubated with nothing in them for 3-4 weeks.

they are just too full of popcorn and the filter patch got wet and sucked something in. sucks but good to know they are comtammed.

so nothing to do with em but pitch em? its cold where im at, they wont survive in a patch outside will they?

 

[canndo]

small holes (bout 1/4") backside slatheried in RTV and a square of tyvek carefully placed and pressed down.(take a little practice cause RTV will come through the tyvek, and you got to watch getting it on your fingers and spreading it all over the patch)

these 2 comtams happened after i shook them they where sterile up till then im sure, remember these jar sat and incubated with nothing in them for 3-4 weeks.

they are just too full of popcorn and the filter patch got wet and sucked something in. sucks but good to know they are comtammed.

so nothing to do with em but pitch em? its cold where im at, they wont survive in a patch outside will they?

If anything touches the fiber filters, things tend to take "root" on the outside and then force their way in. When I do use disk micron filters, I put only the tinyest of holes in the filler disk and that disk goes on before the filter - most times I don't use any air exchange at all - less problems.

 

[testtime]

If anything touches the fiber filters, things tend to take "root" on the outside and then force their way in. When I do use disk micron filters, I put only the tinyest of holes in the filler disk and that disk goes on before the filter - most times I don't use any air exchange at all - less problems.

I used synthetic filter disks. I drill a decent size hole (sorry, just bigger than my needle hole), and then RTV glue a dime size piece of synthetic filter on the top. I put a bead of RTV all around the sides/top of the filter, leaving the center top unglued.

I then flip the lid, and build a lip/bead of RTV on the other side of the filter hole. It ends up leaving a little cone of it around the drill hole. It dries in and hour and I'll do another pass to build the cone. When I shake, I can shake hard, and the grain will bounce off the cone without touching the filter material.

I used this design to keep sharp metal edges from the drill hole from touching the grain, and it grew from there.

No contams in jars in a LONG time, except AFTER I used it for GLC and turned it upside down with 30ML of extra water in it. That's when I started using latex tubes with polyfill for my GLC jars as well as my LC.

 

[canndo]

I used synthetic filter disks. I drill a decent size hole (sorry, just bigger than my needle hole), and then RTV glue a dime size piece of synthetic filter on the top. I put a bead of RTV all around the sides/top of the filter, leaving the center top unglued.

I then flip the lid, and build a lip/bead of RTV on the other side of the filter hole. It ends up leaving a little cone of it around the drill hole. It dries in and hour and I'll do another pass to build the cone. When I shake, I can shake hard, and the grain will bounce off the cone without touching the filter material.

I used this design to keep sharp metal edges from the drill hole from touching the grain, and it grew from there.

No contams in jars in a LONG time, except AFTER I used it for GLC and turned it upside down with 30ML of extra water in it. That's when I started using latex tubes with polyfill for my GLC jars as well as my LC.

And your sure your lc was good? That is usualy why I like to use agar, I can be assured that my innoc, at the very worst has only a spore or two of contam in there. Two dimensions are valuable for that sort of thing, the most loss I ever get is from a spore that landed on the mycelial mass, or a spore that wafted into the system as I was innoculating.

Hey, this is what we work with, the unseen, we have to simply imagine and be creative. I once had a HELL of a time with black pinhead mold - it would grow in bags but only after the first or second shake, I lost over 90 percent until I found a tiny patch of the stuff growing on a wall in the bathroom down the hall - the breeze would pick the stuff up and have it hover around the door of my lab until I walked in - I don't know how it got through the bag filters but what I do know is that when I simply cleaned up that small patch with some alcohol my contamination rate fell to 50 percent, when I did a complete room decontam it went to 0.

 

[testtime]

And your sure your lc was good?

Oh, absolutely. My new jars didn't contam. I'm saying the old jar, the one I put additional liquid in and then shook/swirled and then drew out and used, the OLD jar's filter patch grew green on it. At that moment I decided if I was planning on GLCing a jar, and I wanted to use it for more than a single noc pass, I'd use a latex airport so I could shake and swirl without fear.

 

[polyarcturus]

mycelium is growing slowly, all the good jars are close to fully colonized but not quite there, a couple jars not even close. temps are perfect. semi dark, good conditions. im assuming substrate is getting too dry from being almost 2 months old(3-4 weeks at inoc) what to do to save these? GLC? and G2G the close to fully colonized ones need some help as im about to G2G a jar in a min cause im thinking its gonna start pinning if i dont soon. HELP!

 

[canndo]

mycelium is growing slowly, all the good jars are close to fully colonized but not quite there, a couple jars not even close. temps are perfect. semi dark, good conditions. im assuming substrate is getting too dry from being almost 2 months old(3-4 weeks at inoc) what to do to save these? GLC? and G2G the close to fully colonized ones need some help as im about to G2G a jar in a min cause im thinking its gonna start pinning if i dont soon. HELP!

I think your G2G will work regardless, no matter how dry so long as it isn't dead.

 

[polyarcturus]

i put it off anyways too scared i guess but thats was my plan, PCed some spoons and a knife and a couple syringes and agar while i was at it too thanks for the advice.