How to produce more females from seeds ~

SpaceBuddha

Well-Known Member
Greetings fellow tokers!

I'll be mostly quoting and summarizing here what I've read from an interesting article, hopefully this can help us all work towards an even increased ratio of females from seeds.

The general concept here is that the sex of a cannabis plant is not completely predetermined, and a good grower can make a difference on the female/male ratio by providing an optimal growing environment. :leaf:

"As many marijuana growers know cannabis seed banks are not responsible for male to female ratios. If you get a pack of all males, as many of us have done, then a quick call to your marijuana seed bank can maybe help procure some good substitute freebies with your second order. However some will not go along with this. It depends on your relationship with the marijuana seed bank. Give them a call anyhow, that is my advice, but be discreet and don’t blame the cannabis seed bank or marijuana breeder."
"Feminised Cannabis Seeds"
Courtesy of Dutch Passion

In November 1998 we introduced our "Female Cannabis Seed". We did this after our own experiments showed that from female cannabis seed, we acquired almost 100% female off-spring.

In the meantime we are six months further on. We have received a lot of feed-back from our customers. The reactions are mostly positive! Clients have successfully produced almost 100% female off-spring. However there have been reactions from customers who found a few hermaphrodites or males plants.

Apparently environmental influences affect the sex of the female cannabis seeds as well. Because of the fact that Female marijuana seeds do not grow into female marijuana plants under all circumstances. Thus we changed the name from "Female Cannabis Seeds" into "Feminised Cannabis Seeds".

From literature and from our own findings it appears that the growth of a male or a female cannabis plant from marijunaa seed, except for the predisposition in the gender chromosomes, also depends on various environmental factors. Not only the origination of entirely male or female marijuana plants is partly affected by these environmental factors, the number of male and female cannabis flowers on a hermaphrodite plant is affected as well.
The desired change(s) in the environmental factor(s) are started from the moment that the marijuana seedling has three pairs of real leaves (not counting the seed-lobes). This is the moment that male and/or female predisposition in florescence is being formed. After approximately two weeks the standard growing system can be reconverted to.
The key is to supposedly provide as stress free environment as possible for the 3rd to 4th week, get your babies transplanted to their final pots preferably sooner rather than later to avoid stress.

At the 3rd to 4th week of vegetative growth make sure that your marijuana plants are free from stress. No pests attacks, no fungi attacks, no mold, no irregular photoperiod, not underwatered, not overwatered, not pruned or topped, a cannabis friendly soil mix, not recently transplanted, no small pots. If have these basic growing conditions under control then we can move onto the real forces of female production from standard weed seeds.
Then what are the key factors in the making of optimal environment for females?
the top 3 of the 5 mentioned factors are the most practical:

  1. Level of nitrogen and potassium of the seedbed: A heightening of the standard level of nitrogen makes for more female cannabis plants originating from the weed seeds. A lowering of the nitrogen level shows more male cannabis plants. A heightening of the level of potassium tends to show more male marijuana plants, while a lowering of the potassium level shows more female marijuana plants. A combination of a higher nitrogen level for the period of a week or two and a lowering of the potassium level is recommended.
  2. Humidity and moistness of the seedbed: a higher humidity makes for an increase in the number of female cannabis plants from weed seed, a lowering for an increase in male cannabis plants. The same is valid for the moistness of the seedbed.
  3. Level of temperatures: lower temperatures make for a larger number of female marijuana plants, higher temperatures for more male marijuana plants.
  4. Colour of light: more blue light makes for female cannabis plants from seed, more red light makes for more male cannabis plants.
  5. Hours of daylight: few hours of daylight (e.g. 14 hours) makes for more female individuals, a long day (e.g. 18 hours) makes for more male cannabis plants.

Now let me just make a few adjustments here to this. You can do whatever you want to your cannabis plants in seedling stage and early vegetative stage of growth and it will not effect your final male to female ratios. The time when things should be near perfect is in or around the 3rd to 4th week of vegetative growth.

This is the CRITICAL TIME for getting those female ratios up and up. I realized this clearly when noticing how some cannabis plants hermed because of problems that occurred around this period of the cannabis plants development. If the problems occurred before this time - no herms. So for this reason I surmised that this is when the crucial gender selection is made by the marijuana plant.

Now I believe that the genders are set in the weed seed however the environment has a massive impact on how this is expressed in the final phenotypic expression of the cannabis plants gender. There are probably many genes that govern this, however lets get into how to up these female ratios.
:leaf: N:P:K

What this simply boils down to is that you have the right nutrients present in the right ratios. A nutrient formulation that has roughly equal parts N, P and K is great but if the P levels go up or the N levels go down you are starting to look at a flowering type food for cannabis. If you do this then your odds of producing mostly females is greatly decreased. Make sure that you get those N, P and K levels to almost run from higher to lower amounts from N to P and K.

I have noticed that equal portions of N to P an K can help with the female ratios but the higher N is certainly more helpful. So around the 3rd of 4th week of growth make sure that the ratios are good and that P or K has not gone above the N and P or else more males will occur. Obviously this means to avoid overfeeding your cannabis plants around this time too.
:leaf: Water

Never let your medium dry out completely around the 3rd to 4th week of vegetative marijuana growth. If you make sure to water occasionally, but not to overwater your cannabis plants, you will get those female ratios on the up and up. Overwatering or drying out of the medium will only produce more males. For consistent results in getting more females keeps those mediums moist.
:leaf: Humidity

Now this is the tricky one. High humidity levels only promote fungi and mold development and lowering humidity levels is the way to cure most of this rot but by keeping those humidity levels up in or around the 70 rH factor will help to produce more females. If you have a low humidity grow room then you should get to hold of a humidifier. Now high humidity levels like 70rH cause the medium to dry out a lot quicker so you got to keep this under control too. Keep those mediums moist and those rH levesl at 70. This will help to improve those female to male ratios. Again, getting them on the up and up.
:leaf: Heat

If you run the 24/0 photoperiod then do not allow those temps to go anywhere above 85F unless you have an equatorial strain. 75F is the best but going a little lower is not a problem for helping those female ratios. If you can get in at around 65F then those females are going to be popping up all over the place. The problem with this is that some growers like to use the 18/6 photoperiod and when the lights are off the temps drop from around 65F to 50F and even less. Try not to be below 55F because this has the adverse effect on the plants producing more males than females. Again between 65F and 75F is where you want to be during the 3rd to 4th week of vegetative growth, the preference being 70F.
:leaf: Light

Invest in a MH Light for vegetative growth. Dump the HPS bulb for flowering later. I have noticed that HPS lighting during vegetative growth simply sends those males to female ratios all over the place. With MH lamps the females are everywhere. Invest in some MH HID lights. It makes all the difference in getting those females to show more often. This is worth repeating! MH Bulbs produce more females under optimal conditions especially if they are present during the 3rd and 4th week of vegetative growth. Surprising enough you can start seedlings under HPS and it will not have an effect on those female ratios. Again the 3rd to 4th week of vegetative growth is what is important here.
No stress during the 3rd to 4th week of vegetative growth. That is all there is to it. If you got your garden growing in optimal conditions without marijuana plant stress then the impressive 90% to 95% females start to emerge from standard cannabis seed packs. I find that topping is best done at the second to third week of vegetative growth but that this is a little stressful and can lead to those female ratios dropping again. Avoid topping or pruning if you are looking to up the female count.
All I claim is that this is very interesting information and I personally will apply parts of it in my current grow in hopes of increased female ratio. :weed:

Thoughts? :eyesmoke:

Source:
http://www.1stmarijuanagrowerspage.com/how-to-improve-female-male-ratio-of-cannabis-plant.html
 

TsmokeTrain

Well-Known Member
This is very probable, during my 2 short years of growing i noticed that cannabis plants tend to turn hermy or atleast produce male pollens when put thru stress, i never really had any other stress symptoms then a plant turning herm or showing sex changes.
You might be on to something here,
Peace
 

SpaceBuddha

Well-Known Member
Yes I've noticed in many grows where male/herm rates are up, the conditions are far from stress free.

Of course many would blame the seed bank, but there probably is more to it than that.
 

Trousers

Well-Known Member
Having produced feminized seeds, I believe that the sex of a plant is determined in the seed and not by the environment.
Properly made feminized seeds produce 100% female plants.
 

botanistjjj

Active Member
Having produced feminized seeds, I believe that the sex of a plant is determined in the seed and not by the environment.
Properly made feminized seeds produce 100% female plants.
i agree with this. ive stressed the fuck out of some fem seeds got 4/4 females 1 herm
 

SpaceBuddha

Well-Known Member
Having produced feminized seeds, I believe that the sex of a plant is determined in the seed and not by the environment.
Properly made feminized seeds produce 100% female plants.
Not even the top seed bank feminized seeds give out 100% females every time, fact is all sexes can appear... but we both know fem seeds have a tendency to produce more females.

i agree with this. ive stressed the fuck out of some fem seeds got 4/4 females 1 herm
I've had 100% female rates with 'regular' seeds in far from optimal environment, anything could happen I guess.


Perhaps feminized strains can take 'more of a pounding' and withstand more stress yet still turn out females just fine.
 

Trousers

Well-Known Member
Not even the top seed bank feminized seeds give out 100% females every time, fact is all sexes can appear... but we both know fem seeds have a tendency to produce more females.
What are you basing your opinion on? Properly made feminized seeds are 100% female. They can not be male as they lack a Y chromosome.

Again, I have produced thousands of feminized seeds. Every single plant has been female. There has not been one "hermie."

Besides my experience, biology backs me up. If you take a female plant, that plant will have 2 X chromosomes. (XX)
If you take that plant and use colloidal silver to activate hormones that produce male flowers, you still have a plant that is female (XX). If you use that pollen on another female plant (XX) there is no Y chromosome. How can you get a male plant from that?


COindy said:
I've had 100% female rates with 'regular' seeds in far from optimal environment, anything could happen I guess.


Perhaps feminized strains can take 'more of a pounding' and withstand more stress yet still turn out females just fine.
No. A plant from a feminized seeds is the same as a female plant from regular seeds. Both plants have 2 X chromosomes.

I can not find any solid evidence that environment is a factor at all in sex determination in cannabis.
the fact that feminized seeds exist and do indeed produce 100% females leads me to believe that sex in cannabis is determined by the seed, not the environment.
 

Trousers

Well-Known Member
If you want to increase the amount of females, buy or make feminized seeds.
It is not hard and feminized seeds will produce 100% females.

A gardener with limited space should buy feminized seeds. There is no reason to raise males that are just going to be tossed.
 

SpaceBuddha

Well-Known Member
What are you basing your opinion on? Properly made feminized seeds are 100% female. They can not be male as they lack a Y chromosome.
I'm basing my opinion on countless grow reports, first hand experience, and the information released by dutch passion (here follows the 2nd quote in this thread, incase you hadnt bothered to read it)

"Feminised Cannabis seeds"
Courtesy of Dutch Passion

In November 1998 we introduced our "Female Cannabis Seed". We did this after our own experiments showed that from female cannabis seed, we acquired almost 100% female off-spring.

In the meantime we are six months further on. We have received a lot of feed-back from our customers. The reactions are mostly positive! Clients have successfully produced almost 100% female off-spring. However there have been reactions from customers who found a few hermaphrodites or males plants.

Apparently environmental influences affect the sex of the female cannabis seeds as well. Because of the fact that Female marijuana seeds do not grow into female marijuana plants under all circumstances. Thus we changed the name from "Female Cannabis seeds" into "Feminised Cannabis seeds".

From literature and from our own findings it appears that the growth of a male or a female cannabis plant from marijunaa seed, except for the predisposition in the gender chromosomes, also depends on various environmental factors. Not only the origination of entirely male or female marijuana plants is partly affected by these environmental factors, the number of male and female cannabis flowers on a hermaphrodite plant is affected as well.
Here's more info from the dutch passion website:

Feminised seeds have been one of the most important contributions to the cannabis growing movement in the last generation. Dutch Passion were the first ones who, against all odds, created seeds that gave rise to 95%+ female plants. These seeds are known as feminised seeds, today they are the most popular and convenient choice for those growing their own stash.

Dutch Passion invented feminised seeds and have perhaps the worlds best selection of top quality feminised strains. If you are thinking about buying some feminised seeds then first take a look at what Dutch Passion has to offer – you won’t be disappointed!

In a Dutch Passion experiment done in 1999 Dutch Passion grew 15 varieties of "feminized" seeds. We started with 30 seeds per variety. The goals were: 1) to determine the percentages of female, male, and hermaphroditic plants. 2) to compare the uniformity (homogeneity) among plants from "feminized" seeds with those grown from "regular" seeds.

1. The results were excellent. Nine out of fifteen varieties had 100% female offspring. Percentages of female plants from the other 6 varieties were between 80 and 90%. These plants were all hermaphrodites, producing their male flowers at the end of their lifecycle. Seed-setting hardly took place. No males were found.


2. Approximately 70% of the plants of varieties grown from "feminized" seeds were far more uniform than plants grown from "regular" seeds of the same variety. About 20% of the varieties were a little more uniform, while in 10% of the varieties no difference in uniformity was seen.

From literature and our own findings it appears that the growth of a male or female plant from seed, except for the predisposition in the gender chromosomes, also depends on various environmental factors. The environmental factors that influence gender are:

• a higher nitrogen concentration will give more females.
• a higher potassium concentration will give more males.
• a higher humidity will give more females.
• a lower temperature will give more females.
• more blue light will give more females.
• Fewer hours of light will give more females.

It is important to start these changes at the three-pairs-of-leaves stage and continue for two or three weeks, before reverting to standard conditions.

To produce our feminized seeds, we start with selected female clones. Under standard conditions these female clones do not produce any male flowers. By the method we found, we are able to have these female clones produce abundant male flowers and pollen (see photos). The pollen thus produced we use for the production of our "feminized" seeds.
Source: http://www.dutch-passion.nl/en/grow-info/feminised-seeds/
 

Trousers

Well-Known Member
In case I hadn't bothered to read it?

I have read that. It has been repeated all over the place. That does not mean it is true.
There is no evidence besides stories.

(link to other growing website deleted due to "it's against the rules")

I still haven't found anything that leads me to believe that environment influences sex in seeds after germination.

I would be more sympathetic to the idea that environment plays a role in determining the sex of the seed while it is being produced.

There is a company that claims to be able to determine the sex of a cannabis plant 7 days after germination.
If that is true, Dutch Passion's claims are dubious at best.

3.) Polymerase Chain Reaction PCR and Gel electrophoresis are biotechnology tools for genetic analysis. Montana Biotech has created a PCR based test for determining cannabis marijuana seedlings sex 1 week after seed germination! Cannabis sex is determined by X, Y chromosomes, kinda like humans. This test allows a technician to test for the presence of female or maleness in marijuana seedlings before even the most seasoned cultivator can tell!

http://montanabiotech.com/category/best-method-for-sex-determination-of-cannabis/
 

growone

Well-Known Member
though i'm not sure it qualifies as 'properly feminized', i have been playing with some seeds from a selfed plant. it was spontaneous unfortunately, i.e a hermie
that said, i have just had a male pop out from one of these seeds
which i found to be quite perplexing, MJ one very tricky plant
 

tomahawk2406

Well-Known Member
though i'm not sure it qualifies as 'properly feminized', i have been playing with some seeds from a selfed plant. it was spontaneous unfortunately, i.e a hermie
that said, i have just had a male pop out from one of these seeds
which i found to be quite perplexing, MJ one very tricky plant
i had a greenhouse fem seed...... 1/4 of the plant hermied and spit out tons of seeds. I have about 30 of them and ive planted 6. All six were hermies.
 

tomahawk2406

Well-Known Member
though i'm not sure it qualifies as 'properly feminized', i have been playing with some seeds from a selfed plant. it was spontaneous unfortunately, i.e a hermie
that said, i have just had a male pop out from one of these seeds
which i found to be quite perplexing, MJ one very tricky plant
Thats weird........alot of my knowledge with growing is shit i've experienced. While the internet has taught me tons.......... Its still the internet.
 

growone

Well-Known Member
Thats weird........alot of my knowledge with growing is shit i've experienced. While the internet has taught me tons.......... Its still the internet.
well put, take it all with the grain of salt
i ran this by on another site, lots of interesting thoughts
one was that the hermie i had was actually a male hermie that fertilized itself
i've read from DJ Short, like others, that the fertile male is supposed to be very rare
but if you're not testing the actual genetics in a lab, it becomes a matter of faith of what the hell you have
 
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