This myth arose from early work on plant growth regulators, called auxins, which were mixed with vitamin B-1. Further research throughout the last half of the 20th century investigating the application of auxins to root systems suggested that auxins may stimulate root growth, but that vitamin B-1 on its own does not. Delving deeper into manufacturer claims, many rely on early research (late 1930s and early 1940s) which has been refuted by subsequent research and/or research done on plant roots excised from the mother plant or grown in artificial media (without soil).