Cloning Plants By Tissue Culture

readysetgro

readysetgro

Member
I have to figure out how to post pics lol, im on my phone so maybe it doesnt offer it in mobile version, but i have taken plenty so when i figure out how to post ill be sure to post a bunch. Also, so i CAN tc autoflowers, now THAT is cool. I have 60 day wonder & world of seeds lowryder kush going right now, gonna take plenty of samples!!! Also harvested my bbbc its been about 4 days in a huge paper grocery sack & the stems arent "snapping" yet, (48g) newho; is it safe for me to put it in the jar yet? Normally after 2 or 3 days its ready to be jarred, I harvested sun/mon. Its friday...sn...if i dont get it in a jar its good as vaporized cuz were smoking off it daily wet still lol...
 
pharmacoping

pharmacoping

Active Member
safe....properly dried(before any jars, or none at all) marijuana wil lose about 75% of it's(water) weight while drying. Although it can be used earlier, othing gets bagged or jarred until stems are loud snappers, /or you culd weigh your fresh cut(or even the largest cola drying, and when it weighs 75% less, its ready to cure or use. Most of my product is demanded before it ever sees a jar, and some mj gets nasty after sealed, like hay, useless for smoking, extracts only. Jars and curing made many garden products smell like pocket piss weed, acrid, which is very simply mold, very common ! Good weed will be good weed after about a week in a proper drying room. The hanging nets work awesome and mine is in a controlled room, not the garden, inside a grow tent. Your finished buds, spongy, crisp, dense, will be able to be stored in a vac sealed bag indefinately, but never in the freezer or refridgerator. I've sealed up samples for more than 2 years and when opened they smelled like a fresh harvest.
The drying process is also a chemical one, carbs, starches, cbd, cbn are all being changed into more psychoactive compounds.

If it doesnt smell good, or taste good, or get you high after a week of drying properly, it never will.

If it does smell good, taste good, and get you high, bring some over, and we'll share a :peace: together.
:weed:.
 

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readysetgro

readysetgro

Member
Stems snapped! & Jarred. And just since my first 12 hour "burp" it smells way more "sharp". I think it had something to do with most the buds not being on the stems (im revegging) as to why they didnt dry fast as usual. Just a theory..were getting high smoking it wet!! is that not normal? I know bagseed reefer isnt any good till its cured (to me) smells good tastes good...no high (I have a homegirl that insists that what she finds in the bag was once some "purp or kush" & was ruined from being in the "mexiwild" so she grows them & trys to identify their strain & just calls them what she thinks they are lol. So she has diesely fuelly smelling sativas, minty smelling indicas but they dont get me high!!! Theyll roll 5 or 6 blunts back to back to have a "little" buzz. Do u think its bc its wet & the cure incites the potency or genetics?
 
pharmacoping

pharmacoping

Active Member
While classic experiments have demonstrated that plants are able to adjust the timing of their flowering in response to environmental conditions, such as light, temperature and the availability of nutrients, very little has been known about what exactly triggers plants to make flowers instead of leaves, under various environmental conditions.
Now, a team of researchers from the National University of Singapore (NUS) has discovered how this happens. The team, led by Associate Professor Yu Hao from the Department of Biological Sciences at the NUS Faculty of Science, has identified a protein that is essential for flowering under normal light conditions. The team's findings are published April 17 in the online, open-access journal PLoS Biology.
To identify the element that triggers the process of flowering in plants, Prof Yu and his colleagues undertook a study that spanned around five years, in which they scanned for proteins in plants using a process called yeast two-hybrid screening. After scanning around 3 million samples, the researchers identified a molecule they dubbed FT-INTERACTING PROTEIN 1 (FTIP1).
The researchers found that plants with mutant, non-functional versions of the FTIP1 gene flowered much later under normal light conditions (around 16 hours of light per day). When such mutants were given a working version of this gene, their flowering time was restored largely back to normal.
These findings suggest that FTIP1 is key to how flowering is controlled by light and imply that FTIP1, and genes similar to it, could be used as molecular markers for both classical plant breeding and for targeted genetic modification for desirable flowering traits, with the aim of increasing crop yields in changing environments.
 
pharmacoping

pharmacoping

Active Member
readysetgro said:
Stems snapped! & Jarred. And just since my first 12 hour "burp" it smells way more "sharp". I think it had something to do with most the buds not being on the stems (im revegging) as to why they didnt dry fast as usual. Just a theory..were getting high smoking it wet!! is that not normal? I know bagseed reefer isnt any good till its cured (to me) smells good tastes good...no high (I have a homegirl that insists that what she finds in the bag was once some "purp or kush" & was ruined from being in the "mexiwild" so she grows them & trys to identify their strain & just calls them what she thinks they are lol. So she has diesely fuelly smelling sativas, minty smelling indicas but they dont get me high!!! Theyll roll 5 or 6 blunts back to back to have a "little" buzz. Do u think its bc its wet & the cure incites the potency or genetics?
Click to expand...

when the trichs are milky, the thc is ripe, when its amber, it is degrading. ripe is more energetic, and amber is more sleepy, generally. Weed does not need to be cured, and the "sharp" smell you detect is called acrid, and it is the beginning of spore formation/acid ph value
 
pharmacoping

pharmacoping

Active Member
V

Vincent VonBlown

New Member
Flavor, is a component of "Dank" and it is a hard thing to capture for the most part... Most genetics don't have the ability to be very dank. They will range from Ditchy woody tastes, to sickly sweet. It is very rare to have classic flavors that stand up to the taste of actual food, like say fruit for example.
 
V

Vincent VonBlown

New Member
Real dank, "dank", like the Wonder Thunderstruck clone, (which is where T99 is produced from). You can cut a bud that isn't even done, microwave it. And it still will have a decent flavor. You can't do that with very many strains I'll guarantee you that :)
 
jackblaster

jackblaster

Active Member
pharmacoping said:
yep, besides making a plants bioluminesce successfully, cloning/storing autoflowers has been my funnest experience. In vitro they'll slow vegetation down, to a near stop, divide, sprout, root normally, and I constantly use my santa maria auto mom in a test tube !
Click to expand...
Can I see the biolumeplants you created? do you have photos?
 
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