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Never EVER try to recover anything from jars or spawn. All you will be doing is causing further problems further on down the line. And btw, if ten jars represent "a lot of work" you are either doing something seriously wrong or you have an odd idea of work. Start over dude. Sterilize your...

Again, I rarely use my hood. All you need to do is isolate. If it's your lc you could allow some tissue to rise to the surface and wait, if it is contaminated with mold, you will see discoloration as the spores mature. If it's bacteria you will smell it or the liquid gets cloudy. Let me...

You can start by isolating the source of infection. Do a half a dozen jars and don't inoculate them. Then inoculate with what you usually do but on agar, same procedure.

On the other hand, I had a large amount of mycelium "duff" and refluxed it with alcohol. I came up with a light brown yellow gum that was extremely high in psychoactives. A ball the size of a Pea was over an eighth in strength. Problem was they were very sticky, I rolled them in Wheat...

I was thinking the same. I didn't see any weight references. We know how they multiply things. Figure $60 an eighth or more and then add it all up.

You will NEVER be able to filter your way to contam free beds. The very best way to deal with trich is to use a casing layer and put your ph outside of the trich parameters while still in your mushroom parameters. A well colonized substrate that has been been ph balanced and stays healthy...

I got very lucky. I worked for an electronics assembly house. They damaged a top flow 4 ft table and were about to toss it so I got it for free and only had to replace the damaged element.. but it is loud.

I don't have the nerve to do g2g, even before a hood.

I have to Amend that. I have perpetually diluted a spore solution till I get a spore or two per mil and inject two or three mills into a small batch of lc. That limits my phenos and after fruiting I don't get all those freaks, late fruits and poor pin sets. I've isolated two that do not...

Interesting, I rarely use my hood anymore. Save for agar isolation, every procedure is done in open air. It's all needle to port. Fresh needles each time.

I have always vasilated etween the two techniques. If I am not ABSOLUTELY certain my lc is not contaminated I test it on a dish... but then I have risked contamination in the lc again. Three tiny cubes of agar, for me, only pushes back colonization by some three days. I find that mycelium...

Ok, I've Aldo enriched my lc with peptone and a dash of dextrose. It usually has the effect of making good and gobs of mycelium. Then I will spin up the jar and draw 60 ml. Then I set the syringe needle side down and let it sit. You get hyper concentrated mycelium in the first 20 MLS...

Right away but I use a big shot of culture, 30 to 50 cc. That way I rarely have to shake again.

I do it at three days. You can beat this mycelium up a lot before you might, might kill it.

Here is an example. My wife and I took a slightly larger than museum dose (2 grams about) and went to, you guessed it, a museum. We were very happy seeing all the statues and paintings until we got to the room where the 1930's and 40's German antisemitic art was. It sort of harsher our buzz.

Something odd will Always emerge from multi spore innoculations. There are millions of spore in there and combinations happen, each with a different expression. These aren't crosses. The only way you can know for sure is if you isolate one compatible spore from each pheno and then get clamp...

Cubes have 4 sexes, only two will create clamp connections so one needs to put the correct pair together. Who knows how many natural phenotypes might occur from one load of spores from a single cap but I might try again

Thanks for the clarification. I tried diluting a spore solution down to e Where I got germination at two or three points in each dish and transplanted them to dishes with a single germination of another "type". Spores often clump together so a fresh germination of what looks like a single...

Yeah, it says it is. Now how would you go about accomplishing this cross?

First off, crosses between "strains" are next to impossible. It is highly likely that who ever is claiming a cross is bullshitting you. And second, the jury is still out as to whether different phenotypes offer different experiences.