I have never tissue cultured anything, or grown marijuana, or infringed upon any patents. Everything I say is a lie and every photo I post has been photo shopped a million times even. just sayin'
dude, I apologise for being elusive. This is delicate for me, and I simpy cannot give you the answer I know you're looking for. but it is in the book you said you already read, maybe read it again, or google. when you find it, you can post it, but I cannot.
first i smoke 2 joints. then i take a nice plant... about 4 feet and take off all of the lateral maristems and leaves so that there is only one apical maristem shooting straight up. then i cut it out of the pot and whoop the shit out of this WolfZen character with it.
rinse and repeat.
man forget about these guys. im in the sit. i want to learn to micro-culture but these guys aren't sharing shit. best to read as much about as you can and learn about it another way..... so far im still in the process of building hood with gloves and filters and and positive pressure and all that. first you got to learn the science part then build a small lab that can preform more functions than micro-culture than start experimenting getting the chemicals the you can experimentally micro-culture
this is the process im going about to get there i cant see any other way to do because you cant just jump right into it. even if you know all the ingredients to add to a culture it wont survive if the conditions are not correct. so i guess my idea is if you can build the environments and make the chemicals then you can culture.