pharmacoping
Active Member
take the cleanest piece without mold, quickly rinse in 2% bleach, then sterile water, then transfer. take several excisions just in case. then wash the original when you're sure some of the others will survive.
Cloning Plants By Tissue Culture
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pharmacoping
Active Member
the moldy part is the one with the callus attached silly. The callus is the one with the mold attached
only you can tell
only you can tell
pharmacoping
Active Member
dip scalpel in alcohol, flame off, then cut that callus, maybe use tweezers(flamed) to help and you wont have to transfer the original piece out of the jar. hold your breath, do it fast.
try to get a root included when cutting, but watch that mold goo stuff.
try to get a root included when cutting, but watch that mold goo stuff.
pharmacoping
Active Member
definately some roots there !!
when you can, ditch the candle, and get a alcohol burner(cheap @ amazon)
when you can, ditch the candle, and get a alcohol burner(cheap @ amazon)
Pharm, So now i have cells growing and in my blue media that i made for shoots i now have shoots. What do I do next? In my red agar i made for roots it seems its making a callus then making roots. How would i go about taking a small cutting that has been making shoots and getting some roots on it?
pharmacoping
Active Member
well, first you have to decide what your goals are. If it's just new fresh cuttings, then scalpel a chunk off of any material that is growing roots/sprouts. Put the rooting calli into the sprouting mix, and the sprouts in the rooting mix if their slow. Then for a cutting, take a piece of callus with roots(at least) and root them like you would a clone, either in rockwool, or into another tube without hormones, but with sugar.
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pharmacoping
Active Member
can even use a commercial rooting compound at this stage .
no thanks necessary, my jollies are gotten watching your success ....your first time out !!
no thanks necessary, my jollies are gotten watching your success ....your first time out !!
ok here is 1 ...chem dog 91...i have a callus forming at the bottom like around the trunk. I'd like to get to the point where when i need 100 cuttings i can make them that is my goal. So do you recommend just making a lot of callus's? this one is in the shooting formula. But i'm kind of confused...my thinking when i did this was the roots would grow in the agar just like how you would take a cutting and it would form roots. But seems even my rooting formula it makes a callus then roots off of that..is that correct? if so then i would take that rooting callus and place it on top of the agar but in my shoot formula to make a shoot? Once that is done i then take the whole rooting/shooting callus and plant like normal? after letting it aclimate to the climate?
pharmacoping
Active Member
you are exactly correct. your root mix is a little hot, evedenced by the curling leaves, but thats ok. the rest of your plan is right on.
At this stage callus material would be your goal, however, experimenting with a little less hormones would be helpful. Your mix will work for sure,
but may have some mixhaps. sometimes I expect roots and get shoots instead, its ok, both can happen in a rockwool cube too.
great pic !!
At this stage callus material would be your goal, however, experimenting with a little less hormones would be helpful. Your mix will work for sure,
but may have some mixhaps. sometimes I expect roots and get shoots instead, its ok, both can happen in a rockwool cube too.
great pic !!
pharmacoping
Active Member
reducing the hormone levels by half never hurt me, when I saw symptoms. If your salts are at or under 150ppm they should be fine, if not, adjusting them a little will expedite your growth, and lessen the chance for a freaky failure
readysetgro
Member
Intresting thread, I have been following it from the beginning all morning. Are there any more picture updates to those that have been posting? I will be purchasing my materials to build a lab this wknd, & hopefully be proceeding to tc next week or so thanks to al the info ive found here! Never has it been made as plain for me. Anywho I plan to tc some big buddha blue cheese & some th seeds s.a.g.e. I also have some stalks from some local strawberry cough that a friend saved for me. Im most excited about these bc the stems are a year or so old & no longer green, so i cant wait to experiment on bringing them back to life 
Great thread, great info i will have plenty of ?s & lots of pics coming up! Thanks!!
Great thread, great info i will have plenty of ?s & lots of pics coming up! Thanks!!
jujubee
Active Member
I think you will need live plant matter to tc.
I haven't read the whole thread yet. I've had some tc gear for a little while, but haven't got around to trying it out. I'm still not sure on the hormones. I have NNA, IBA, BAP and Kinetin. I also have agar, ms and ppm. Is there something else I should have?
I haven't read the whole thread yet. I've had some tc gear for a little while, but haven't got around to trying it out. I'm still not sure on the hormones. I have NNA, IBA, BAP and Kinetin. I also have agar, ms and ppm. Is there something else I should have?
readysetgro
Member
The strawberry cough stems are rehydrating now. Week 2. Theyre in an experimental setup Ive got going, pretty much a dwc bucket. One of them is visibly starting to show green, most of the others just "feel" like theyre going from sticklike to live plant stem like but arent showing any visual signs. I read in a Skunk a yr or so ago how to tc & they mentioned taking specimens from dead plants. Either way i never planned to just try to tc a peice that i hadnt rehydrated but i think i will now for control purposes. Its all experimental though