UseScience
New Member
I thought since there seems to be a lot of confusion in the threads I'd try to help out.
Silver Thiosulfate – Plant Tissue Culture Protocol
Prepare a 0.1 M Sodium Thiosulfate (STS) stock solution by dissolving 1.58 g of Sodium Thiosulfate (Product No. S7026) into 100 mL of water. Prepare a 0.1 M Silver Nitrate stock solution by dissolving 1.7 g of Silver Nitrate (Product No. S7276) into 100 mL of water. Store the stock solutions in the dark until needed to prepare the STS.
In general, the STS is prepared with a molar ratio between silver and thiosulfate of 1:4, respectively. Nearly all of the silver present in the solution is in the form of [Ag(S2O3)2]3-, the active complex for ethylene effect inhibition.
Prepare a 0.02 M STS by slowly pouring 20 mL of 0.1 M silver nitrate stock solution into 80 mL of 0.1 M sodium thiosulfate stock solution. The STS can be stored in the refrigerator for up to a month. However, preparation of the STS just prior to use is recommended.
I've used it numerous times. Best time to spray is just as you switch the plants to flower, using a fine misty ensuring coverage, and once again 7 days later. In my experience this is all that is necessary. Using too much and overly soaking the plant material will kill the tissues. Less is more.
It. Works. Every. Time.
You can get the shelf life of the component chemicals from the Google's. Store them in the fridge unmixed for months. I've used the combined mixture for up to 2 months(refrigerated).
If a mod could pin this, that'd be cool too <3
Silver Thiosulfate – Plant Tissue Culture Protocol
Prepare a 0.1 M Sodium Thiosulfate (STS) stock solution by dissolving 1.58 g of Sodium Thiosulfate (Product No. S7026) into 100 mL of water. Prepare a 0.1 M Silver Nitrate stock solution by dissolving 1.7 g of Silver Nitrate (Product No. S7276) into 100 mL of water. Store the stock solutions in the dark until needed to prepare the STS.
In general, the STS is prepared with a molar ratio between silver and thiosulfate of 1:4, respectively. Nearly all of the silver present in the solution is in the form of [Ag(S2O3)2]3-, the active complex for ethylene effect inhibition.
Prepare a 0.02 M STS by slowly pouring 20 mL of 0.1 M silver nitrate stock solution into 80 mL of 0.1 M sodium thiosulfate stock solution. The STS can be stored in the refrigerator for up to a month. However, preparation of the STS just prior to use is recommended.
I've used it numerous times. Best time to spray is just as you switch the plants to flower, using a fine misty ensuring coverage, and once again 7 days later. In my experience this is all that is necessary. Using too much and overly soaking the plant material will kill the tissues. Less is more.
It. Works. Every. Time.
You can get the shelf life of the component chemicals from the Google's. Store them in the fridge unmixed for months. I've used the combined mixture for up to 2 months(refrigerated).
If a mod could pin this, that'd be cool too <3
