germination using colchicine solution

Loki- thank you for the heads up man...............

merry pranksters, huh?

good times..

i have never heard of anyone dying from smoking buds from plants treated with this substance.
but thats not saying much, as ive also never heard of anyone that has successfully used this technique for breeding purposes....
i can see how the plant could be toxic with such high levels of Colchicine present... but what i was wanting was to create Colchicine treated parents, breed them, and then cross the f1'a with an ibl, and see what happens. however, with no proper analyzing equipment to test the end f1 hybrid product for levels of contamination, so I fear that for me, this is quickly becoming a pipe dream....

also colchicine can be found in medicine used for the treatment of gout.
extracting it, isolating, and then administering the roper concentrations though, is an entirely different story...

I spoke with a grower with over 30 years experience. He said they did it in the 80's. He soaked the seeds in a solution, but only 10% would germinate. He said you can smoke those since the tiny amount they absorb will produce polyploidism but not effect the buds. If you were to treat the plant later it would be poisonous. He said many of the males were sterile. Sounds very interesting.
Daniels

thats kinda what i was wanting to do daniels...
kinda like induce the polyploid trait into dominance, then try to keep that trait apparent in the offspring.
make them ladies thick and bushy... multi- cola plant w/ no fim or topping involved...

He did say they were noticeably stronger, but of course it is a mutation so many were fucked up growth patterns. I think you gave me again for a testing experiment to look into. I'll look into some colchicine solution from my uncle a farmer/rancher. He may know a source & have credibility to get it. I'll just have to do 10 seeds at a time if I try it maybe less.
BTW how the Hell have you been? Did you get a vert. RDWC Heath style going?
Daniels

i was set back when i lost my winter run due to nematodes
so instead i punted and started growing out some moms.
ive got the rdwc designed, just got to build it.
when my moms get to where i can take 18 cuttings every 18 days im going to build it

Come down with the gout if you want to procure colchicine, but be very careful with the stuff. You can also crush the bulbs of the crocus flower to obtain it, but good luck with that. Also, you can't smoke the first generation when grown using this stuff, you have to wait for the clone generation, preferably the second clone generation.

I actually have gout, & they prescribed Indomethicin. But that link earlier is still active and it was $60. I will look for a local source or ask my uncle. Still a ways from even the research & planning stage. Plus I will need a few dozen seeds from a cross I do.
Daniels

I got a hold of some Colcemid...its a substance similar to colchicine, but less toxic. It is commonly used in cytology to prepare karyograms because it arrests exposed cells in metapahse (when the chromosomes are most easily distinguishable). Has anyone else used it for creating polyploid plants? Would it work?

If anyone is interested I have the book "The Connoisseur's Handbook Of Marijuana".

I'll make a torrent file for it and re-post in here again with the hash for the torrent and a link on where to find the torrent file.