Let there be white: mc130p's horticultural adventure continues

The Dawg

Well-Known Member
honestly, i don't think i'll get a pounder but let's let dawg worry. also, i've never grown this strain before, i don't know anything about it haha.
Really now am I the only one rooting for ya? You know us cob growers has to stick together :hump: :clap: bongsmilie
 

mc130p

Well-Known Member
Kinda bored. Here's an overlay of three color fluorescence microscopy of human stem cells. By using a cocktail of growth factors, I can differentiate these cells into glutamatergic neurons...only takes about a week for them to completely change their shape. Action potential firing is observable after about 2 weeks. The ER is labeled with antibodies for Sec61b and a chimeric Sec61b protein that I designed. The nucleus shows fluorescence from nuclear localized mNeonGreen. Scale bar is 50 micrometers.

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Here's another image of the endoplasmic reticulum in stem cells. This time I used antibodies against another protein I made and the protein called Vesicle Associated Membrane Protein Associated Protein B, VAPB
1617792135163.png

Here's an image of mitochondria in stem cells labeled with antibodies against my chimeric construct and the outer mitochondrial membrane protein TOMM20.
1617791837687.png
 
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