Marijuana Tissue Culture Success!

In Michigan your heating/cooling bills will negate a full time grow. Culturing in an atmosphere between 70-100%RH, and temps ranging 40-130F will be challenging for sure. There are millions of spores outside that don't exist indoors, especially in an unused closet. Mites love greenhouses too, and these will find your succulent cultures, and bring mold with them. take some pics please, I love greenhouse grows. I miserable failed at a clear covered 30x40 pole barn in our state. I could not heat it properly in the winter, and cooling the magnified sun rays was impossible, for me. I buried manure, ran solar fans/filters, used solar heating, passive and active solar hot water heating, and even 1300 gallon tilapia tanks in a recirculating ebb/flow system. The building now has a steel roof, and is a true living bio dome all year, cooled/heated for under 500 month.

pharmacoping,
Can you help me put together a good medium to start?

and how you add pictures here...?

never mind I just figure it out... lol

420SC said:

pharmacoping,
Can you help me put together a good medium to start?

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no he will not, hes stuck up, just kidding

but luckily for you i have some thing for you to try
i just purchased "marijuana from test tubes" off of amazon.com
i dont recommend purchaseing it. its $25 and the only exclusive info
are the two recipes im about to give you for free.

here are some 1 liter formulas outlined in the book

Conclusions: For the home hobbyist, the most reliable protocol is as follows:
Stage I from cuttings/ Stage II divisions:
1 MS with vitamins
1 ml PPM
.5 ml IBA at 1mg/ ml
1 ml Kinetin at 1mg/ ml
9 g/ l agar
30 g/ l sucrose (household sugar)

Stage III Rooting:
1 MS with vitamins
1 ml PPM
1 ml IBA at 1 mg/ ml
1 ml TDZ at 0.1mg/ ml
9 g/ l agar
30g/ l sucrose (household sugar)
0.5g/l activated charcoal (0.7/ l = ¼ teaspoon for home measurement purposes).

we should be culture buddys. i recently moved into a house and have a whole room dedicated to sterile transfers(just mushroom culture at this point). so i will be free to experiment soon.

it the literature it mentioned that a ph of 5.6 was ideal

heres a weird one, hey pharmacoping, do you get shoots with seed leaves from your callus cultures?

heres an article on synthetic seeds that im sure few have read

http://link.springer.com/article/10.1007/s12298-009-0008-8

what are "seed leaves" ?

Unfortunately neither of those recipes will ever allow full healthy expression . Surely you can see the importance of that book you purchased, especially for people never knowing of this? Those recipes are readily available for free on google, and actually pretty standard in the industry. Even the book itself is free for anyone knowing the google game. I love that book, and if I would have had it 30 yrs ago, my plants would be talking by now !

oxanaca said:

no he will not, hes stuck up, just kidding

but luckily for you i have some thing for you to try
i just purchased "marijuana from test tubes" off of amazon.com
i dont recommend purchaseing it. its $25 and the only exclusive info
are the two recipes im about to give you for free.

here are some 1 liter formulas outlined in the book

Conclusions: For the home hobbyist, the most reliable protocol is as follows:
Stage I from cuttings/ Stage II divisions:
1 MS with vitamins
1 ml PPM
.5 ml IBA at 1mg/ ml
1 ml Kinetin at 1mg/ ml
9 g/ l agar
30 g/ l sucrose (household sugar)

Stage III Rooting:
1 MS with vitamins
1 ml PPM
1 ml IBA at 1 mg/ ml
1 ml TDZ at 0.1mg/ ml
9 g/ l agar
30g/ l sucrose (household sugar)
0.5g/l activated charcoal (0.7/ l = ¼ teaspoon for home measurement purposes).

Click to expand...

mine are lighted for 24 hours with a small 100 watt flouro t5 unit that fill the whole culture tent with good light.

pharmacoping said:

what are "seed leaves" ?

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oops Cotyledon. i call them seed leaves. you know the first two round leaves that form after you germinate a cannabis seed. they usually dry up after the plant grows a few nodes.

cant you see them in the picture. there clearly showing in the shoot protruding from the piece of callus. here ill post it again in case your computer didnt show it.

pharmacoping said:

mine are lighted for 24 hours with a small 100 watt flouro t5 unit that fill the whole culture tent with good light.

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what color temperature do you use. how many umoles of ppfd do you recommend i expose my culture vessels to, this study has information on the subject. although im not sure whether they got there readings on the inside or outside of their culture vessels

http://link.springer.com/article/10.1007/s11627-008-9167-5

you dont think they need a rest period

pharmacoping said:

Unfortunately neither of those recipes will ever allow full healthy expression . Surely you can see the importance of that book you purchased, especially for people never knowing of this? Those recipes are readily available for free on google, and actually pretty standard in the industry. Even the book itself is free for anyone knowing the google game. I love that book, and if I would have had it 30 yrs ago, my plants would be talking by now !

Click to expand...

i dont think were on the same page. im refering to marijuana from test tubes not "Plants from test tubes". it came out the last day of last year

have you tried either recipe, which part of the formulation do you have a problem with.

http://www.amazon.com/Marijuana-Test-Tubes-Micropropagating-Cannabis-ebook/dp/B00FK133XU

ive owned plants from test tubes for about 3 years. and i definitely recommend buying that its an excellent introduction to micro propagation.

last post for now i swear. i looked for a micropropagation formulation for carrots and was unable to find one. you wouldnt mind posting this would you.

I've tried something similar to this

brick20 said:

I've tried something similar to this

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and the result was?

just thought id drop in and share some more worthwhile links.

this place has some very interesting info including info on synthetic "clone" seeds
http://skunkpharmresearch.com/tissue-culture/
+ the info he used
http://home.olemiss.edu/~suman/Directshootorganogenesis.pdf

this guy managed to produced some of the prettiest invitro shoots ive seen so far. he even gives away his recipes, something a lot of groweres wont do.
http://www.shroomery.org/forums/showflat.php/Number/10803981#10803981

Is dr tissue culture still around or anybody know the company website?

icdog said:

Is dr tissue culture still around or anybody know the company website?

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Just throwing this out there, but if you want to buy some lab equipment for tissue culturing there's plenty of less-shady companies online you can find through a Google search - even per-assembled tissue culturing kits, hormones, etc. if you don't know where to start. But good luck getting from differentiated cultured tissue (not hard) to an actually viable plant growing on it's own when it comes to cannabis and a home lab.