My Clone From Leaf Experiment

Bain

Bain

Well-Known Member
ultranyte said:
yeah I guess a little rooting hormone might help... but i dont see what a few vitamins is really gonna do to force rooting from a source that doesn't have genetic instructions to form roots.... lol
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To quote and/or paraphrase from Woomiester, who - if nothing else - knows more about botany than I do:

"Once the healthy Fan leaf is placed into its growing medium the blade continues to produce IAA(auxin)...it accumulates and forms a callus tissue...These cells have no determined fate and as such can differentiate in all directions...[until] a concentration is reached that roots are formed"

I removed some of the scientific names that can confuse, but the general idea is there that the leaf produces cells that can turn into anything, even roots.

I don't know much about this stuff but if nothing else I believe it can happen in some plants.
 
potroast

potroast

Uses the Rollitup profile
It may be possible to grow roots, but what will grow? A plant grows from the growing tip, called the meristem.

HTH :mrgreen:
 
MsMILFweed

MsMILFweed

Well-Known Member
Well 24 hours later and they're still alive. It's nice and humid in the dome.....

I'm kinda disappointed I haven't heard from Woo in this.. I presume I'm doing it right??
 

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MsMILFweed

MsMILFweed

Well-Known Member
TetraHyC said:
Well they're still alive, not wilted.

Heres a pic I took this morning.:roll:

Found a little more info on the subject.:confused:

Cutting (plant) - Wikipedia, the free encyclopedia
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Interesting link ..


Leaf cuttings, in which a leaf is placed on moist soil. These have to develop both new stems and new roots. Some leaves will produce one plant at the base of the leaf. In some species, multiple new plants can be produced at many places on one leaf, and these can be induced by cutting the leaf veins.


That seems to be the technique we're trying.
 
TetraHyC

TetraHyC

Well-Known Member
Only thing that photo shows roots coming out of the petiole.

I thought were trying to get roots to sprout from the veins.

It seems the veins and the stems have similar structure and function.

Hmmm.
 
primeralives

primeralives

Well-Known Member
Hi, glad to respond to someone who accepts the unknown is possible when you know how!requirements as follows:
  1. heated propagator
  2. growing medium( I use potting compost for this technique)
  3. A healthy fan leaf from as lower branch as possible(5 fingered and cut as close to the central blade as possible)
  4. rooting gel
  5. scalpel
  6. patience
Cut the selected fan leaf from a healthy plant with as short a petiole(stem) as possible; just long enough to remain in contact with the growing medium. With your scalpel very carefully cut half way through the veins of the seperate blades on the underside of the leaf(you can dip your scalpel in rooting gel beforehand if you wish) and rub rooting gel into the wounds. Place the leaf ( stem in medium) flat on the growing medium and devise a way of making sure the leaf veins remain in contact with the medium without blocking the shoots from emerging from the 'cut sites'.Keep in heated propagor at 70oC and mist daily. I use Cfls on 20/4 as I have found they are happier with a rest(arent we all!) This method creates five shoots from each leaf which in purists terms are actually five seperate plants; but can be trained as one. If you grow hydro then make sure you fill the end of one leg of a pair of womens tights with the compost(to make a large jiffy pellet almost) which can be transfered into expanded clay at a later date then the compost eventually washes away with the flushing cycles( I use Flood and drain). The rooting will take up to 2 weeks, so you need patience. The main advantage I believe is the negation of need for 'Mother' plants so optimising growing space. Good luck and any queries drop me a line.

that was a pm i got from woo b4 everyone scared him off the site by doubting and shitting on him

 
nowstopwhining

nowstopwhining

Too many brownies
MsMILFweed said:
Well I decided to start a new thread for this rather than clutter up Woomeisters already really long thread on this topic
https://www.rollitup.org/advanced-marijuana-cultivation/60994-cloning-fan-leaves.html

I'm not sure if I've done it right, so hopefully Woo will chime in and correct me.

Here's my details for the pics.

#1 - Got my fresh picked leaves (Northern Lights and American Dream), cloning gel (Rootech), some fresh sealed scalpel blades and a tray of medium (Fox Farm blue bag soil).

#2 - One of the leaves of American Dream close up (nice red/purple veins eh?)

#3 -Getting ready to cut the vein with gel on the blade - I cut through the main vein (trying to take a little wedge out on a couple of them), a couple of them I ended up cutting right through the leaf. Eeek!

#4, 5 & 6 - various shots of the cuts with gel

#7 - the leaves in the medium. - The leaf on the left is American Dream and the one on the right is Northern Lights.

They have then been placed in my humidity chamber in the cab.

Is this the correct way?
Do you think it's gonna work?
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*edit* sorry I had no idea this was serious.

I will be around waiting for the results.
 
O

oldskoolstyle

Active Member
Actually it is well known that growers can clone a fan leaf... in fact somebody did it in a thread around a year ago on this site....

The leaf will grow roots it just won't grow into a plant... or if it does eventually produce shoots it just takes too long and therefore becomes not worth the effort.

Although recently I have heard that it still might be possible if you use certain chemicals to induce the leaf to produce shoots.
 
TetraHyC

TetraHyC

Well-Known Member
I PMed Woo asked him to come back, I said we won't jump his shit anymore.
I think he's English, I saw some price he gave in pounds.
 
Woomeister

Woomeister

Well-Known Member
MsMILFweed said:
Well 24 hours later and they're still alive. It's nice and humid in the dome.....

I'm kinda disappointed I haven't heard from Woo in this.. I presume I'm doing it right??
Click to expand...
yes looks good , the only difference is I personally weight the leaves down with chicken wire to keep the leaves pressed aginst the soil.
For the non-believers out there who cant possibly believe the earth is round,the new shoots appear directly above the cut sites of the veins. Someone made a silly comment about leaves not having genetic makeup to grow into plants -WRONG! This method is also nothing like tissue culture it is a simple AVP method(artificial vegetative process) and has been used in my area for years. There are several methods and this is just the most successful one that I have shared.
 
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