My first trip down the rabbit hole...

I am following your thread. What type of poo recipe are you pondering on? I know that you have the horsey doo-doo. But what, if any other components do you intend to use? Also. Are you going with just the monotub, or are you going to put a case on it?

Someguy15 said:

yea I am ready to spawn to poo. I know about g2g but have to be more sterile and syringes only last so long so I figured I'd just use them up. I will prob g2g any strains I want to keep around and then just print anything else for storage. A well stored print can last over 5-10 years.

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Someguy, that is easy to say but I have tried to revive 5 year old prints with only very slight sucess. When after 20 days I saw no germination I began to try different teqniques. I soaked the spores in water for a week and got a few germinations. I placed the spores in water AND a vaccum and got What I suppose might be a slight increase. When all was said and done, I abandoned the print and started with new ones. The spore steadily dehidrates and shrinks and it will not germinate unless it is hydrated. I know for a fact that old spores will germinate but it takes more doing than smply placing them back on agar. Now I have been reading about fully desicated mycelium - even desicated mushrooms being rehydrated and growing again but I have never confirmed this.

Thing is I have a shitload of pets, even useing a glovebox is no guarantee here...
So I can choose between less pets (and I have one of those fancy vacuums that filters through water, sound like a bong) or less contams.

Oh, I FAILED with just a glove box. A damn fine one, but still, not enough room, flames were kinda dangerous, fogs made it blind, on and on.

I mean take a 4 foot section of a closet wall. Put a rack of shelves there. Seal 100% around it, using vinyl on the front. Cut out / add in gloves pretty much everywhere that you need to. Make sure you can handle needles and move big things (full pressure cookers) between the shelves.

Then seal in that total area that you are standing in. I use large amounts of clear plastic and duck tape. Cut/adhere zipper doorways. Then do it again to the front of the closet.

You CAN keep 100% clean a small area. Just choose it and seal it up.

It's doable. One just needs the space for it. If not. Just keep doing what you've been doing. As long as you do. What you want to do.

yeah I'm going to do a glove box for now until I have the space for a hepa laminar flow hood. As long as I can get 75% success I'll b happy. WBS and jars r cheap enough.

Ok. Someguy. When are you going to do an LC (liquid culture)? This will cut down on your costs considerably.

DaSprout said:

Ok. Someguy. When are you going to do an LC (liquid culture)? This will cut down on your costs considerably.

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When I get some fruits? Dont you have to use fruit to make a LC? (ok so I'm still learning) Honestly, maybe growing has made me see things different, but the actual per round costs, even buying spores and waiting, is still so low I hardly care either way. Eventually yes, I would love to learn to do everything, but baby steps. I'm going to try for a first successful harvest before I worry about 'perpetual' or anything like that. If I can get a 1/2lb of shrooms this will be successful, and I will really have no need to do another run...at least not for 6 months or so. Just have to see how it works out, if it's fail back to drawing board... but I think learning to prep wbs and how to spawn to bulk will save me a ton of headaches vs the PF cake tek. Only part I can see going wrong now is if things fail at grain transfer. But I'm assuming since it doesn't require a sterile environment it can't be too hard. Key seems to b making sure your grain is well covered as any uncolonized grain is an open invite for a contam.

Just checked on the spawn jars. 6/7 have activity! Both Penis envys are going great. As are the hawaiians and TC. Only been about 91 hours since inoculated, so fucking stoked right now. This put the rye berry bags to SHAME already, can't wait to see what happens in a week. Pics as soon as I get my camera back...shit maybe I should just go buy one lol

Sounds good. And all you need is the last cc's of your syringes to make a liquid culture. When you use a small piece of actual fruit, usually from the inner stem, that is a clone culture.

Well the latter seems more useful, its an isolation right? so not MS?

No an LC can still be multispore, but it will be maybe two or 3 variants, yeay they are weird, they grow into each other. and even useing a wedge from well inside the stem is no gaurantee of an isolate. That I'm afraid can only done with certainty on agar plates. Haveing said that, L/C's are realy easy to make, and a good way of storeing DNA for a while. I posted a recipe useing coffee rinds and sugar...

Since you're only going to do this for a run or two. You probably wouldn't want to waste any time on an LC.

DaSprout said:

Since you're only going to do this for a run or two. You probably wouldn't want to waste any time on an LC.

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That would depend upon how many jars/bags one was innoculating and how heavy the intended innoculation.

ANC said:

No an LC can still be multispore, but it will be maybe two or 3 variants, yeay they are weird, they grow into each other. and even useing a wedge from well inside the stem is no gaurantee of an isolate. That I'm afraid can only done with certainty on agar plates. Haveing said that, L/C's are realy easy to make, and a good way of storeing DNA for a while. I posted a recipe useing coffee rinds and sugar...

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wow... I cannot believe that taking a tissue sample from a single fruit is not an isolation. That's hard to wrap my head around. Ok so say you have 3 "phenos" (spores) since the mycelium can connect as a single network and then fruit, it's possible for the fruit to contain genetic material from multiple "phenos" or spores? Fuckin crazy.

And liquid culture is cool, but beyond my needs right now. Only made 7 quarts and 2 3lb bags and I think I'm already gonna have too much spawn.

Also this may seem like a dumb question, but under a scope could one physically manipulate the spores to isolate a single spore for inoculation? My intiution tells me they are too small to manipulate but shit they can fertilize eggs by injecting them and crazy shit so who knows.

And picked this up to expand my knowledge, great book so far.

Good stuff going on man. I used to have that stamets book. I think it was the mushroom cultivator or something. I was reading it like crazy. But that was over 10 years ago I believe. I love this mushroom thing. I'm getting back the fever. I'm even thinking of a green grow some time in the near future. Probably months from now though.

Someguy15 said:

wow... I cannot believe that taking a tissue sample from a single fruit is not an isolation. That's hard to wrap my head around. Ok so say you have 3 "phenos" (spores) since the mycelium can connect as a single network and then fruit, it's possible for the fruit to contain genetic material from multiple "phenos" or spores? Fuckin crazy.

And liquid culture is cool, but beyond my needs right now. Only made 7 quarts and 2 3lb bags and I think I'm already gonna have too much spawn.

Also this may seem like a dumb question, but under a scope could one physically manipulate the spores to isolate a single spore for inoculation? My intiution tells me they are too small to manipulate but shit they can fertilize eggs by injecting them and crazy shit so who knows.

And picked this up to expand my knowledge, great book so far.
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You needn't do it that way at all. Dilute your spore solution, then dilute it again, then squirt a cc or so on a plate, you will find only a few points of germination, you can isolate each germination point onto a new dish and you will have a single entity mycelial mass, then you can do what you like with each one. BTW - great book, but get his first one as well - they are companion pieces - the second book is mostly about domesticating various species - species by species.

TC:


PE:


GT:


Hawaiian:

Cool man. Looks like everythings moving along. I'm just waiting for my casing to be ready to fruit. Maybe a few more days for me.

I would give them another 2 or 3 days before shaking them up, should be ready by sunday. And Congrats on 7/7!