abso1utepain
Well-Known Member
ok so i've got a few questions for anyone that can answer
first one, i've colonized some BRF cakes i have 4 that are about 90% colonized, and 2 that i ran low on mix so they were only 2/3rds as full as the other jars(100% for almost a week and moved into fruiting chamber), no issues with sterility so far
BUT my 2 jars in the fruiting chamber won't fruit. i did the dunk and roll(after 3 8 hour soaks(submerge, refrigerate, rotate repeat), they are in the rubber made container with an inch of perlite on the bottom, the cakes are sitting on cds so they stay out of the perlite, and i have a 20 gallon aquarium air pump pushing fresh air into the perlite to introduce new air and increase humidity(might put it in a bowl of water see if that works better. i'm averageing 70% humidity, it has gotten down to 55% once. temp is between 68-80, fluctuates with the room temperature. any idea why i have no fruit after 2 weeks?
(strain was B+)
i also have 6 jars of a puerto rican strain, but i ran out of foil for the last jar, so it was steralized with just a lid and holes drilled. then once innoculated put in a ziplock bag....
oddly enough this jar has about 10 times the growth of the foal covered lids
innoc. points of foil lids have growth about the size of a dime(3 spots around the jar) the jar in a bag has 1.5 inches of growth down and has circled the top of the jar already.
any ideas?
thanx
first one, i've colonized some BRF cakes i have 4 that are about 90% colonized, and 2 that i ran low on mix so they were only 2/3rds as full as the other jars(100% for almost a week and moved into fruiting chamber), no issues with sterility so far
BUT my 2 jars in the fruiting chamber won't fruit. i did the dunk and roll(after 3 8 hour soaks(submerge, refrigerate, rotate repeat), they are in the rubber made container with an inch of perlite on the bottom, the cakes are sitting on cds so they stay out of the perlite, and i have a 20 gallon aquarium air pump pushing fresh air into the perlite to introduce new air and increase humidity(might put it in a bowl of water see if that works better. i'm averageing 70% humidity, it has gotten down to 55% once. temp is between 68-80, fluctuates with the room temperature. any idea why i have no fruit after 2 weeks?
(strain was B+)
i also have 6 jars of a puerto rican strain, but i ran out of foil for the last jar, so it was steralized with just a lid and holes drilled. then once innoculated put in a ziplock bag....
oddly enough this jar has about 10 times the growth of the foal covered lids
innoc. points of foil lids have growth about the size of a dime(3 spots around the jar) the jar in a bag has 1.5 inches of growth down and has circled the top of the jar already.
any ideas?
thanx