Test tube growing

i got my plant tissue culture almost all set up to begin a trial. i've been reading for 2 weeks. bought a book on it and read some more. i found lots of info at the library website for my city so i'm going to get some books on plant biology and advanced cultivation techniques. i got the jars to incubate and grow my tissues in, i have the agar and glucose and distilled water to make the growing medium gel. i have the indole-3 butyric acid and naphthalene acetic acid for the auxins which are the plant growth hormone for rooting. i just need to get the cytokinin for rapid cell division and shooting. once i have that i can take my meristem samples out of being in suspended animation and introduce the cytokinin to start cell division and induce shoot growth. once that happens just put into new medium with the auxins iba and naa to grow roots from the shoot. i'm so excited. i have all the sterile stuff i need and the incubator and sterile environment. keeping everything clean is going to be my biggest challenge. just one tiny mold spore or any other cell lands on my shit then it will replicate way faster than the tissue sample and it won't grow. i haven't much money invested in this project, i'd say about $30 tops.one big benefit i am convinced on why this method is the best way to reproduce a plant asexually. traditional cloning methods cause the clones to become weaker and weaker the more you veer from the mother. plant tissue rebuilds the individual cells. and rebuilds it making it more resilient and hardier and better yielding plants.check it:

whoa...someone needs to goto college for botany

YES!!!! I am looking everywhere around my area in so cal.. i do so much reading. i love plants in general. just everything they do for us and how we have no idea how complex they are.

dude seriously that some advanced shit im expecting one or two people to know about this stuff on here and one is now you! lol

thats cool as hell.
"traditional cloning methods cause the clones to become weaker and weaker the more you veer from the mother"
can you show me where you found that? I've heard it before but never saw any proof at all and in fact seen people with perpetual grows who do clones of clones each generation for frequent harvests who say its a myth. I've got an open mind on the subject I'd like to read more about it.

thanks!! i wish i knew more! there is so much more to learn. i never liked school back in the day but now i want to go back. im only 23 i still can. just need to find a place who has what im looking for. there are greenhouses all over so cal that have their own tissue labs that do their own cultures. be kick ass to do something like that. but need a degree first uuuugh but im more motivated about this than anything. i already done all my car stuff i wanted to do. thought that would be a good job but eh.

darkdestruction420 said:

thats cool as hell.
"traditional cloning methods cause the clones to become weaker and weaker the more you veer from the mother"
can you show me where you found that? I've heard it before but never saw any proof at all and in fact seen people with perpetual grows who do clones of clones each generation for frequent harvests who say its a myth. I've got an open mind on the subject I'd like to read more about it.

Click to expand...

directly from my book my friend...
As long as you can keep a clean area you can tissue culture. TC (tissue culturing) helps regenerate the plant cells by multiplying them. When you clone with traditional methods you harm the plant and over time the plant is no longer able to produce fruit as well as it use to. With tissue culturing it rebuilds the plants cell structures to make it stronger and healthier to produce a better fruit. Compared to a mother plant they are mini versions of adult plants and grow to it environment. Unlike cloning you use less water, less light and less maintenance. It does take a little more time to do the actual TC but there is hardly any maintenance once you are done. In the end the plants yield more and are fuller greener and healthier.

that book seems biased towards tissue culture and promoting it. I was hoping for something more substantial more focused on the clones degrading over a few years time when taken the normal way and yeild and quality suffering.

well im sure there is more info. im just starting to learn. this only the beginning, i seriously hope. i have a friend who graduated from cal poly pomona with a degree in advanced botany. we dont talk much but maybe we can now. never had much in common until now i guess.

just got another book. its called plant propagator's bible. and plant propagation with tissue culture. i read a whole 200 page book in about a day. that is how serious i am. heheh i got my list of supplies left i need. i figure about one more trip to a few places.got my jars,got my tray, forceps,scalpel,got my clean transfer chamber ready to transfer and prepare tissue samples in. have many many literature on it. just need some more money. hah..



also i learned you can tissue culture a seed. you sprout seed like normal and once it has its tap root you take that and put into a jar and medium containing hormone to grow shoots and the stem cells on the tap root will grow shoots and branches like a clone. all before u even grow the plant. FUCK! how COOL! u can grow take one seed and grow a few shoots from it then cut them off and grow roots in another test tube. then flower normal to determine sex all before the plant is even grown. then if its a female you can continue with culturing it and making thousands of plants.