Tissue Culture/Micro Propagation Grow from Scratch

budforever442200

budforever442200

Well-Known Member
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researchkitty said:
Let me know when you have a bud. Till then its sprout that just wont grow new tops. That's where everyone before you has failed. Now please dont take my comments negatively, I hope someone makes a success. Yours isnt yet, its just where everyone else left off after failing. :)
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But they are growing shoots, here is the same plant 1 day after culture, 5 days after culture, and 7 days. Sorry the pics are right to left.
 
researchkitty

researchkitty

Well-Known Member
I dont think anyone doubted they were yours, you've been a member here for nearly 4 years now, it'd be a weird turn to be faking it lol.............. :)
 
canndo

canndo

Well-Known Member
researchkitty said:
Let me know when you have a bud. Till then its sprout that just wont grow new tops. That's where everyone before you has failed. Now please dont take my comments negatively, I hope someone makes a success. Yours isnt yet, its just where everyone else left off after failing. :)
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This is not at all true, I posted dozens of pictures of plantlets that grew buds and transplanted buds that grew more buds. What I have failed to show you so far is rooting in vetro and hardening off back to a standard grow. You will see this come fall. Seems that I am not the only one now. My experiments are fine but until another person can duplicate the work it is still all bulshit
 
canndo

canndo

Well-Known Member
Budforever, you are not hijacking my thread, but it would be nice if you generaly describe your protocol.
 
S

Sandbagger

New Member
canndo said:
This is not at all true, I posted dozens of pictures of plantlets that grew buds and transplanted buds that grew more buds....
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Do you have a link to these. Is it in another thread ? I am new here and missed this. I have yet to see shoots grow out and believe me I have searched.
 
S

Sandbagger

New Member
I read this from page 1 and did see some shoots, but I did not see any rooted or flowered. I would love to see it happen though.
 
canndo

canndo

Well-Known Member
You will. I have rooted but could not show pics because the roots are obscured by the media. Eventually I will flower in vitro for kicks.
 
itsallinthewrist

itsallinthewrist

Active Member
this is very interesting i have never heard of it before but will check into it more it intrigues me that you can do such a think but anything is possible i believe (as gay as that sounds) you are a gentlemen and a scholar canndo
 
canndo

canndo

Well-Known Member
This is not an ended experiment, I contracted pneumonia and could do nothing but post on this site and sleep for several months. I have the new chambers ready, I have (well I will soon have) access to plenty of raw material - something that was stopping me before I got sick. Thankis itsall.
 
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