trichome question

just bought a microscope at radioshack.,things nice..,but my question is Should I cut off a piece of bud to examine trichs?i tried looking at them but its hard without the plant shaking around..

You can cut a piece of or take an entire blade of a small leaf right by your buds or in the case of a cola with leave protruding from/through it you can take a piece of one and lay it on a table or something and check it.

They may not be at the exact same stage of development as the buds themselves but being that close they will at the very least be very close to being the same. Once the trich color on your test leaf piece is close to what you want overall then you can start to check the buds themselves even if that requires nipping a small piece of one.
　
While I have never done it myself I have I known of people who would use something like a piece of 1x1 or 2x2 or a wood rod like a piece of broomstick that was long enough to rest against a wall in their grow area and then hold the other end in one hand and then rest their scope/hand on it to steady themselves … sort of like how people who painted various things that required a steady hand have used.

You need to check trichs in several areas around the plant then sort of take an average. I've seen 50% amber in one place and mostly clear in another. Even on the same leaf it can vary wildly from location to location.

Illegal Smile said:

You need to check trichs in several areas around the plant then sort of take an average. I've seen 50% amber in one place and mostly clear in another. Even on the same leaf it can vary wildly from location to location.

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thanks .,and you were right.., on the same leaf i saw trichs that were clear.,foggy and a couple amber ones too...,im going to flush this week and see how it looks next week..the only thing that is confusing me is the pistils are still 60-70% white or more.., does that matter at all?

i would say they are a little too early then. There is more to consider when determining the overall maturity of the bud than just the trichome color.

yoitsslobo said:

thanks .,and you were right.., on the same leaf i saw trichs that were clear.,foggy and a couple amber ones too...,im going to flush this week and see how it looks next week..the only thing that is confusing me is the pistils are still 60-70% white or more.., does that matter at all?

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Not really. Go by your trichs.

Brick Top is right on.

I snip a 1/2 inch tip off of a trim leaf, (leaf on the bud) and hold it with clean tweezers, and lay it on a white dinner plate and look at it sitting down at the kitchen table. Its not easy at first, but after you learn to look at the edges first, to get it focused, then you can learn how to maneuver around and see it all, even both sides.

Illegal Smile said:

You need to check trichs in several areas around the plant then sort of take an average. I've seen 50% amber in one place and mostly clear in another. Even on the same leaf it can vary wildly from location to location.

Click to expand...

That is very true and is something I had intended to mention but in my normal verbose manner I went on just long enough to forget to add part of what I had intended to say.
　
Like was mentioned plants will mature at different rates, especially if there is inadequate and or unequal lighting.

The upper portion may have a trichome color that you most want and the middle and or lower portion may not be close to what is most desired.
　
So yes definitely check the trichomes at different levels but pay closest attention to the tops because if you pay more attention to the middle and or lower portions your tops will likely over mature.

Contributed by: Snaps_provolone
Submitted June 18, 2003

What are Trichomes?




capitate stalked trichome photo by: Eirik

Although cannabis resin glands called trichomes are structurally diverse, they come in three basic varieties:


Bulbous:
The bulbous type is the smallest (15-30 micron). From one to four cells make up the "foot" and "stalk," and one to four cells make up the "head" of the gland. Head cells secrete a resin - presumably cannabinoids, and related compounds which accumulate between the head cells and the cuticle. When the gland matures, a nipple-like protrusion may form on the membrane from the pressure of the accumulating resin. The bulbous glands are found scattered about the surfaces of the above-ground plant parts.

Capitate-Sessile:
The second type of gland is much larger & is more numerous than the bulbous glands. They are called capitate, which means having a globular-shaped head. On immature plants, the heads lie flush, appearing not to have a stalk and are called capitate sessile. They actually have a stalk that is one cell high, although it may not be visible beneath the globular head. The head is composed of usually eight, but up to 16 cells, that form a convex rosette. These cells secrete cannabinoids, and related compounds which accumulate between the rosette and it's outer membrane. This gives it a spherical shape. The gland measures from 25 to 100 micron across.

Capitate-Stalked:
Cannabinoids are most abundant in the capitate-stalked gland which consists of a tier of secretory disc cells subtending a large non-cellular secretory cavity. During flowering the capitate glands that appear on the newly formed plant parts take on a third form. Some of the glands are raised to a height of 150 to 500 micron when their stalks elongate. These capitate-stalked glands appear during flowering and form their densest cover on the female flower bracts. They are also highly concentrated on the small leaves that accompany the flowers. The male flowers have stalked glands on the sepals, but they are smaller and less concentrated than on the female bracts. Male flowers form a row of very large capitate glands along the opposite sides of anthers.



photo by: Proof_of_the_pudding


photo by: Proof_of_the_pudding
The figures above denote capitate-stalked trichomes with green arrows, the bulbous trichomes with yellow arrows & the red arrows mark the capitate-sessile trichomes. Cyan arrows denote cystolith hairs.

Life inside a capitate-stalked trichome

image by: Snaps_Provolone
Disc cells, attached to leaf or bract by stipe cells (RED) & basal cells (GREEN), release fibrillar wall matrix into secretory cavity where it contributes to thickening of subcuticular wall during enlargement of secretory cavity. Plastids (ORANGE) in disc cells produce secretions called lipoplasts which synthesize quantities of lipophilic substances that accumulate outside the plasma membrane, migrating into the endoplasmic reticular cytoplasm and through the plasma membrane and cell wall into the secretory cavity where they form vesicles (BLUE) in the secretory cavity. Vesicles in contact with the subcuticular wall release contents that contribute to the growth of the cuticle during the enlargement of the secretory cavity. THC occurs in the walls, fibrillar matrix & other contents surrounding the vesicles, but not in the vesicles. Trace amounts of THC is present in the disc cells.


photo by: Eirik


When to harvest your trichomes
There are several schools of thought as to when it is the time to harvest. I shall attempt to explain how you can determine the harvesting time that will produce the most favorable psychoactive effect for your individual preferences.

We are most concerned with the capitate-stalked trichomes, as these contain the overwhelming majority of the psychoactive cannabinoids (THC, THCV, CBN). Different cannabinoids affect the high in a multifaceted manner.

THC:
delta-9-tetrahydrocannabinol & delta-8-tetrahydrocannabinol - THC mimics the action of anandamide, a neurotransmitter produced naturally in the body, which binds with the cannabinoid receptors in the brain to produce the ?high? associated with marijuana. THC possesses high UV-B (280-315 nm) absorption properties.

THCV:
tetrahydrocannabivarin - prevalent in certain South African and Southeast Asian strains of cannabis. It is said to produce a ?clearer high? & seems to possess many of the therapeutic properties of THC.

CBD:
cannabidiol - previously believed to be psychoactive, or to contribute to the high by interacting with other cannabinoids, conversely the most recent research indicates that CBD has negligible effect on the high, it is however a strong anti-inflammatory, and may take the edge off some THC effects, such as anxiety. CBD as a non-psychoactive cannabinoid appears to be helpful for many medical conditions. CBD biosynthesizes into cannabinol (CBN) & tetrahydrocannabinol (THC).

CBN:
cannabinol - a degradation product of THC, produces a depressant effect, ?fuzzy? forehead.

CBC:
cannabichromene - non-psychoactive , a precursor to THC.

CBG:
cannabigerol - non-psychoactive, hemp strains often posses elevated levels of CBG while possessing only trace amounts of THC.

Heavy trichome production is not necessarily an indication of a potent plant. Some hemp strains have moderate layers of trichomes yet pack only a strong headache. In a drug strain, a thick layer of trichomes is a symbol that it may well posses an elevated potency level, but it is certainly not a guarantee.

What defines a cannabis drug strain is the plant's ability to produce THC & THCV.

A small 25x or stronger pocket microscope, which can be picked up inexpensively at an electronics store like Radio Shack, works well for getting a closer peek at your trichome development. We are examining are the capitate stalked glandular trichomes, the coloration of these gland heads can vary between strains and maturity. Most strains start with clear or slightly amber heads which gradually become cloudy or opaque when THC levels have peaked and are beginning to degrade. Regardless of the initial color of the secretory cavity, with careful observation you should be able to see a change in coloration as maturity levels off.

Some cultivators wait for about half of the secretory cavities to go opaque before harvesting, to ensure maximum THC levels in the finished product. Of course nothing tells the truth more than your own perception, so try samples at various stages to see what is best for you & the phenotype your are growing. While you may be increasing the total THC level in the bud by allowing half of the glands to go opaque, the bud will also have a larger percentage of THC breakdown products such as CBN, which is why some people choose to harvest earlier while most of the secretory cavities are still clear.

Indica varieties will usually have a 10-15 day harvest window to work with. Sativas and Indica/Sativa hybrids often have an extended period to work with.


photo by: Eirik


photo by: Proof_of_the_pudding
The figures above denotes clear trichomes with green arrows, the cloudy trichomes with yellow arrows & the red arrows mark the amber trichomes.

Why did trichomes evolve in nature?
Cannabis has evolved trichomes for a multitude of uses in nature, some of these require THC & other cannabinoids to be effective, and others that do not.

Insect Protection:
Many insects find the thick coating of trichomes unpleasant, this offers a level of protection for the developing seeds.

Animals:
The layer of trichomes and cystolith hairs makes cannabis less palatable to many herbivores & omnivores.

Desiccation:
The layer of trichomes helps to 'insulate' the pistilate (female) flower from low humidity levels and high wind.

UV-B Light:
UV-B light is harmful to living things, THC has very high UV-B adsorption properties, thus cannabis evolution may have favored the evolution of genotypes that produced these THC laden capitate-stalked trichomes as a built in 'sun-screen' for protection against UV-B light rays.

Fungal Protection:
Some of the compounds present in the trichomes actually inhibit the growths of some types of fungus.

Quite possibly, the most important reason for the evolution of the THC laden capitate-stalked trichomes is the intercession of man in the natural selection process, favoring genotypes that produce copious amounts of THC laden trichomes.

New growers are in far more danger of harvesting too early than too late, so when in doubt - give it another week.

A little more of the same but said somewhat different and with possibly a few different details:



What are Trichomes? Trichome 101
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Harvesting is, without a doubt, the most exciting part of the wonderful journey that is cannabis cultivation. After months of watching, waiting and tending to your plants’ every need, the time is approaching when you must cut them down to harvest their resin-coated buds and all of the leftover trim that can be used to make fresh potent hash.

You know that you need to harvest your plants in the coming weeks, but when exactly is the best time to do it? Every strain of marijuana has its own life cycle, so it’s not as simple as a specific number of weeks after flowering has begun. It’s even more complicated since you can choose when to harvest your crop for bud that provides either a head high or a body stone. Luckily, most if not all strains of commercially grown cannabis provide visual clues on when to harvest, and what type of high you are likely to achieve.

In this article, we’re going to explore the final phase of your plants’ growth cycle: the flowering or budding phase. Specifically, we want to look at the last two weeks of the flowering phase in what is known as the “window of peak maturity”. The window of peak maturity is when trichome development and the level of THC production in your plants have reached their maximum point, which is when you would harvest your plants. By being patient and paying close attention to certain indicators, you can reap fantastic buds that provide you with precisely the type of high that suits you best.

THC and Trichomes
Almost everyone who enjoys marijuana knows that, for whatever reason, THC is the psychoactive component in the bud they ingest.

As a cannabis cultivator, it helps to know just a little bit more than what THC is—such as how it develops over time and how chemical changes in trichomes contribute to different psychoactive effects.

The primary goal of any plant is to create and nurture seeds to be viable for future growth. Trichomes help prevent seed damage from insects, animals, light degradation and fungal disease. In the case of cannabis, trichomes also contain THC, the chemical compound that provides a psychedelic high to humans. THC stands for Tetrahydrocannabinol (C21H30O2). Near the end of the flowering cycle, THC begins to break down and turns into another chemical compound called Cannabinol, or CBN (C21H26O2), in a process known as oxidization. Higher levels of CBN tend to provide a more narcotic or “stoned” feeling, while THC delivers a more euphoric, upbeat “high”. Knowing this, you can examine the trichomes and the pistils of the plant, then choose when you harvest to get the psychoactive effects you desire.

Cannabis plants produce THC and CBN on their stems, leaves, and vegetation surrounding the buds and are developed in trichomes, which emerge on the surface of most of the plant’s parts. On the stems and the early fan leaves, the trichomes are small and hug the surface. As the flowering phase continues, the glands develop on the more mature parts of the plant, including the smaller leaves and the first calyxes (which exist to develop and nurture seeds if male pollen fertilizes the female plant). The trichomes that develop on calyxes no longer hug the plant’s surface, but are on stalks like mushrooms with bulbous caps. During this time, more and more trichome-covered calyxes develop and create densely packed clusters, called ‘bud’. As your plants enter the final stages of their life cycle, the calyxes begin to swell and ripen, while more and more resin glands develop on the surface.

Another indication of plant maturity is the color of the pistils, the little hairs that grow from inside the calyxes. Their purpose is to collect male pollen to fertilize the ovum inside the calyx, creating a seed. When no male pollen exists calyxes grow dense without any seeds, resulting in sinsemilla cannabis. Near the end of the flowering phase, pistils change color, entering the window of peak maturity. The ratio of white pistils to red pistils determines the type of effects your plants are likely to produce: a high or stone. In the final few weeks of the flowering phase, the pistils change from bright white to a rusty orange or brown, signifying the end of the plant’s life cycle.

Examining Trichomes

As the amount of trichomes covering your plant increases, you should take a closer look at them, maintaining a light touch to avoid crushing the resin glands. Your best bet is to purchase an inexpensive 25x microscope (available at most electronics stores), and most pocket microscopes have a small light to help you get an illuminated peek at your trichome development.

When you cut small buds from your plant to test them, you want to concentrate on the stalked glandular trichomes. The coloration of the gland heads can vary with different strains and maturity, but most start with clear or slightly amber heads that gradually become cloudy or opaque when THC levels have peaked and are beginning to degrade. Regardless of the initial color of the trichomes, with careful observation you should be able to see a change in coloration as maturity levels off. Some cultivators wait for about half of the trichomes to go opaque before harvesting to ensure maximum THC levels in the finished product. However, you will also want to try samples at various stages to see what is best for you. While you may be increasing the total THC level in the cannabis by allowing half of the glands to go opaque, there will also be a larger percentage of CBN, which is why some people choose to harvest earlier while most of the trichomes are still clear.

Now you understand how trichomes develop on your plants and how to examine them as they turn from clear to opaque, indicating THC breakdown.

Early and Late Harvesting

Every strain has its own unique window of peak maturity, typically one to two weeks long. However, there is a difference between har vesting early or late within that window, depending on whether you want a head high or a body high.

As cannabis matures the chemistry of the plant changes, as does the type of effects. While these differences in chemical nature still require a lot of research to fully understand, you can utilize them to produce different types of highs for different needs.

By harvesting earlier in the window your buds tend to produce more of a cerebral head high, an effect much more pronounced in Sativas than Indicas. A head high is more psychedelic in nature, providing you with a pleasant mental “up” state, often ideally suited for social situations when you still need to have your wits and plenty of energy.

If you choose to harvest later in the window, your buds produce more of a body high, which is conversely more pronounced in Indicas than in Sativas. A body high is similar to a narcotic “down” effect, usually associated with being “stoned”. A down type of high is often desired in the late evening to calm nerves and drift into sleep. A late-harvest yield is often sought by medicinal cannabis users to ease pain and increase appetite. If you choose to harvest somewhere in the middle of the peak window of maturity, you don’t necessarily get the best of both worlds, but more of a happy medium. If you aren’t sure what type of high you like or want, aim for the middle and you likely won’t be disappointed.

As you continue to examine your plants, you will be tempted, even compelled, to start pulling buds off of your plants to test them out. There is a right way and a wrong way to try out your buds before they are ready, so let’s take a look at your options.

Testing Your Buds

Most new growers start picking at their crop only a few weeks after budding starts. Usually this is because inexperienced growers get too excited and they don’t know that these small, immature buds still have a way to go before they hit peak maturity and reach the highest trichome development. But there is definitely nothing wrong with being excited about your ladies! Even veteran growers still get giddy seeing their plants start to sag under the weight of so much resin.

I don’t recommend taking any buds from your plants until they are approaching their window of harvest, and you should only take a small bud every day or two. You can test your first bud when approximately 10% of the pistils have turned reddishbrown; take it from the middle area of the plant. Once you’ve cut your self a small bud to try out, you need to dry it, but drying and curing traditionally takes longer than the entire window of peak maturity, so you have to quick dry it. Quick drying is a sub-optimal way to turn a lush, wet bud into something you can smoke, but it’s the best way to quickly test your bud every other day as it matures.

There are lots of different ways to quick dry your bud, but one of the best ways is to use your lighting ballasts in the grow room. First, cut up your fresh bud and spread it out evenly in an envelope. Close the envelope and place it on top of your ballast, then leave it there for two to three hours while the light is on. After the buds are dried out, put them in an airtight container and allow the last of the moisture to move from the stem into the bud. Considering this is a quick dry method, the taste is usually good and the potency is acceptable. Also, the use of a vaporizer will allow you to concentrate on your high rather than being distracted by the odd taste of quick-dried weed smoke.

By now you know what trichomes are and how the chemicals in them evolve from producing a head high to a body high over a couple of weeks. You also know that the pistils on your buds change color from white to reddish-brown as the window of harvest opens and closes, as well as what type of high to expect depending on the percentage of new and mature pistils. Throughout the window of harvest, you took small buds from the middle of the plant, quick dried them, then tested them out to determine the type of high you want your crop to give you. Now, you can harvest your plants with confidence in knowing that they will be loaded with resin that produces just the right kind of high you were looking for. Enjoy your harvest!

Good thread - there can't be enough said about what may arguably be the most important stage of growing, the harvest.

"Buy a scope" isn't the best advice that can be given in this community, and we've all seen outdated and inaccurate information cut and pasted... most of which only leaves most - newbies - scratching their heads. The available guides on harvest just aren't that good.

I'm continue to learn as my harvest approaches, but here are few things I have figured out, with the help of others here, and researching on the internet.

The majority of breeders, experienced growers, and coffee-shop connoisseurs generally look for 30 - 40% amber trichomes. Others like to harvest a little earlier or later, depending on their personal preferences, but most home growers continue to harvest too early. As long as patience is a vertue, I expect this trend to continue.

I have heard complains about weed that has been harvested too early, but I have never heard anyone say "I let it go too long", and that says a lot.

There may be some difference in the amount of time it takes for one strain to ripen over another, and the breeders are known to exaggerate... Sativa's are notoriously slow to ripen under artificial lights.

The pistol argument: It's valid! I don't know of any strain that matures with 50% white pistols still showing. Many strains aren't finished until 90% of the pistols turn and curl-up into the false seedpod, they emerged from, before they are finished! Next time, I'll leave the scope in the drawer until I see the pistols turn...

More on withering pistols:
"Many times you'll read from new marijuana growers, "My hairs are 50% changed to brown or red, looks like its ready to come down." Calyx hairs turning color is one thing, calyx hairs withering, is another. When they have withered, they are for the most part, as ripe as they will get.

But just turning red/brown does not necessarily mean the weed plant is ready.Calyx hairs are just an indicator that the marijuana plant is ripening , not necessarily ripe yet. I have taken full Indica strains with 60% hairs turned, and sativas when 90% hairs have turned, and they were all at peak ripeness. The trichome color is the only true indicator!" source the amsterdam seedbank

I've noticed the tops of my plants tend to finish first, but I see little discussion about staggered harvests - taking the tops of the plant first, and then allowing the lower buds to ripen with the additional available light.

Next time you look in the scope check the upper leaves, the tops, and the lower buds and you'll see the indoor MJ plant doesn't usually ripen all at once. There really is no rush to harvest; if the tops look done, most likely the lower buds aren't even close, and you're better off waiting at least another week.

All comments and discussion welcome

thanks for all the help people..much rep+
ill post pics in about a week

yoitsslobo said:

thanks for all the help people..much rep+
ill post pics in about a week

Click to expand...

this thread is very informative and put together for only 2 pages...

Green Cross you mentioned having a staggered harvest when the tops are much riper that the bottom. However you didn't really say whether it was a good or bad thing. Whats your opinion?