I wanted to ask the same question, when is the best time to run, lights on or off? and also i know that when you run your co2 you want all ventilation off (ducting air out of the room), or it will be wasted. If anyone can answer this also it would be appreciated, (im sure mrmo123) would want to know the same but . . . Once you run the co2, how long should you leave the ventilation off before its safe to turn it back on?