How are you going to decide exactly what material to weigh come harvest? Do you decide or a team of seasoned laboratory professionals and their assistants? What if disease or insect pressure, or some other cultural issues affects some but not others which will affect the outcome and skew the results? /QUOTE]
I am going to weigh, as state in the OP, the final trimmed buds at a uniform RH of 55%. Again, I have been a lab assistant as an undergrad and am paid to work in a lab as a grad student, so I am a lab professional. I wish I had assistants. I am technically familiar with precision. One does not prepare slides of material 2 microns thick without precision. If disease or some other event occurs, I will report on it and discuss it and its affects. If that happens, its ok because I will just repeat the experiment over and over.
Plant tissue moisture levels? You do have an accurate method/instruments to measure moisture levels in the tissue?
At what point in the plant's development will you say part or all of A or B is ready?
At least you have a control group to play with.QUOTE said:
I calculate the VPD using a formula. The leaves of the plant have adequate turgor. A and B may be ready at different times due to the defoliation. If so, it will be reported. However, I plan to harvest all plants within minutes of each other.
Nobody is saying your shouldn't do your experiment. We've pointed out that these "experiments" are a dime a dozen said:
I am not parroting anything seen on the internet. I am doing a simple experiment with a subject and a control in the same environment with the same nutrients. Basic? yes. Scientific? enough for me.
I have not seen anyone do a side by side with n=18. I have seen lots of posts saying "I wish someone would do a side by side defol experiment to see..."
I'm non partisan. I am not trying to prove that defol increases yield. As a trained scientist, I am letting the result speak for themselves. Period. I AM a non-partisan independent lab. I am not producing a study to hype any clients products or anything. This is a quest for EMPIRICAL KNOWLEDGE.
I would think that would be encouraged and supported here.