LOL it's free and I'm good at it.Whiner!
It snowed like fk here for about 5 hours this morning, fortunately it's warm enough not to stick.
LOL it's free and I'm good at it.Whiner!
It snowed like fk here for about 5 hours this morning, fortunately it's warm enough not to stick.
2x4 for cleats is code for a very good reasonI might use 2x4s. I have a bunch of little pieces laying around, not a lot of 2x2. My 1x4s are 10ft for roofing so I'm not sure if I want to cut those up.
Did you offer a respectful farewell to easter?Having a drink and taking care of the scrap from the barn.
View attachment 4323066
I've heard that tissue culture will clean up 'issues' with older clone generation. I honestly don't know but it's something to consider.Another harvest week, very busy. After a decade, I think I'm going to have to retire my Trainwreck strain. It's only producing half of what it used to. When I started growing about 10 years ago (it's weird to write that, that's a while) the current data suggested that cloned plants are identical to its parent. But current data is suggesting the opposite - Current Science Cloning Article
Out of about 15 or so phenos of strains that I've kept and cloned for commercial production, this is only the second one that has accumulated net negative characteristics through the mutations inherent in cloning. The other strain I lost was a gorgeous cut of Northern Lights #5. Everyone mourned that death. I started this TW at the same time as an OG Kush mom that, if anything, has actually gotten better through time (except that it has become a little more difficult to clone.) I have these fast flowering Durban fem seeds that I've yet to pop, if I find a good pheno I think this will replace the TW. Oh well. ♫ Nothing lasts forever, even cold November Rain ♫
Making new moms, so I took a bunch of extra cuts and filled the cloner before culling the old -
View attachment 4323131
New moms. Sorry about the fucked up lines...
View attachment 4323134
Planted these on 4/20, so they should turn out to be Super Moms...
If you could point me to some helpful links, I'd appreciate it. I'm no botanist, but I can't fathom how tissue culture can correct net negative mutations of a phenotype. Gotta look into it, though. I don't want to commit an argument from ignorance fallacyI've heard that tissue culture will clean up 'issues' with older clone generation. I honestly don't know but it's something to consider.
I admire how you name the fallacy you do not wish to fall foul of LOL Your mind always amazes me.If you could point me to some helpful links, I'd appreciate it. I'm no botanist, but I can't fathom how tissue culture can correct net negative mutations of a phenotype. Gotta look into it, though. I don't want to commit an argument from ignorance fallacy
So, in case you were wondering about @420God 's progress, I got some advance info on the slackerI'm putting stairs in my barn so I can use the hay mow for storage. It's a huge area not being used and was only accessible by ladder.
So, in case you were wondering about @420God 's progress, I got some advance info on the slacker
before (last night)View attachment 4323166
after (this morning)
Excellent set-up, kudos. Clean and professional, just like some research labs I've seen.Another harvest week, very busy. After a decade, I think I'm going to have to retire my Trainwreck strain. It's only producing half of what it used to. When I started growing about 10 years ago (it's weird to write that, that's a while) the current data suggested that cloned plants are identical to its parent. But current data is suggesting the opposite - Current Science Cloning Article
Out of about 15 or so phenos of strains that I've kept and cloned for commercial production, this is only the second one that has accumulated net negative characteristics through the mutations inherent in cloning. The other strain I lost was a gorgeous cut of Northern Lights #5. Everyone mourned that death. I started this TW at the same time as an OG Kush mom that, if anything, has actually gotten better through time (except that it has become a little more difficult to clone.) I have these fast flowering Durban fem seeds that I've yet to pop, if I find a good pheno I think this will replace the TW. Oh well. ♫ Nothing lasts forever, even cold November Rain ♫
Making new moms, so I took a bunch of extra cuts and filled the cloner before culling the old -
View attachment 4323131
New moms. Sorry about the fucked up lines...
View attachment 4323134
Planted these on 4/20, so they should turn out to be Super Moms...
Pronounced AOWE, and it is the thing unionist graduates say loudly every time one barks a shin.Lol. He must have attended the @tangerinegreen555 school of Advanced Obsessive Work Ethic. Most never make it out alive...
The cannabis genome isn't that well mapped out yet; one would need to know the specific sequence causing the negative phenotype and the specific sequence of the desired trait(s) before clipping. Arabidopsis is an old, well known and respected experimental system in botany, much like Drosophila is in genetics wherein the genome is well mapped. We aren't there yet with CannabisIf you could point me to some helpful links, I'd appreciate it. I'm no botanist, but I can't fathom how tissue culture can correct net negative mutations of a phenotype. Gotta look into it, though. I don't want to commit an argument from ignorance fallacy
Gotta love those quick-drying finishesSo, in case you were wondering about @420God 's progress, I got some advance info on the slacker
before (last night)View attachment 4323166
after (this morning)
My understanding is a meristem tissue culture is virus free. If causation is genetic drift over time then you need whole sequence genomics. That was my impaired line of thought.The cannabis genome isn't that well mapped out yet; one would need to know the specific sequence causing the negative phenotype and the specific sequence of the desired trait(s) before clipping. Arabidopsis is an old, well known and respected experimental system in botany, much like Drosophila is in genetics wherein the genome is well mapped. We aren't there yet with Cannabis
To expensive and not realistic yet, there are companies out there: http://www.segra-intl.com/cannabis-tissue-culture-production/# so someday.If you could point me to some helpful links, I'd appreciate it. I'm no botanist, but I can't fathom how tissue culture can correct net negative mutations of a phenotype. Gotta look into it, though. I don't want to commit an argument from ignorance fallacy
Exactly, as well to consider telomeric degradation which in Tyler's situation is likely plausibleMy understanding is a meristem tissue culture is virus free. If causation is genetic drift over time then you need whole sequence genomics. That was my impaired line of thought.
LOL Muller's Ratchet, one theory is it's because of that pesky mutant Y chromosomeExactly, as well to consider telomeric degradation which in Tyler's situation is likely plausible
FIFYExactly, as well to consider moral degradation which in Tyler's situation is likely plausible
The bdelloid rotifers seem to have found the button on the ratchet handleLOL Muller's Ratchet, one theory is it's because of that pesky mutant Y chromosome