HLVd destroying cannabis crops

iggy097

Well-Known Member
This was just posted recently - good watch, lots of information and lab tests- their studies show that it is way more possible than thought to spread through seeds -
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Drop That Sound

Well-Known Member
You guy's worry too much about crazy plant viroid epidemics. It spreads fear, and spells uncertainty of the future of cannabis (if it is even real, haha). Gives all the power away to the greedy corporations IMO. OK, i won't go there.. There is no reason to fear :)

Just go get a big clear plastic tub for ten 10-15 bucks to make a still air box to work in, and pickup a a few tissue cultured aquarium plants at petco for cheap. They're on sale, buy 3 get one free. They already did all the work, making & sterilizing the media, etc, for less than $10 lol. Plus you get extra plants with the deal, which you can pull out, or even probably just leave in.

It's like the uncle bens method of growing shrooms with microwaveable 90 second rice packs, which all the "real" mush cultivators seem to hate on, so don't tell anyone your doing it. Or that it was drop that sounds idea ;)

Then, get some 70% ethanol, and some bleach, and make up 3 jars. One with the ethenol, one with plain water, and one with 10% bleach water. In your SAB (study up on how to work in a still air box, I don't have time to slain), cut away the thin non-woody "meristem" sections from the tips of some small cuttings off whatever cultivar you think might be "infected", which you also should have sprayed/soaked in bleach water before bringing in the SAB. Jut bring in enough material to take the samples from, on its own clean dish. Use forceps\tweezers to dunk the sample piece you cut (with a clean razor) first into the ethanol solution, then the water to rinse that off, then finally into the bleach water, in that order.

Then, carefully open up the petco tissue culture plant container (you should have cleaned it up too with bleach water first) and stick the sample down into the gel, right next to the other plants you bought, lol. Don't go waving your hand all over it, be methodical in the way you work inside the box, trying not to contaminate the tissue culture media container, or stir up the air.

Put the lid back on. Wait for it to grow out. Something like that anyway.

Then, worry about how we faked the moon landing instead.

Take that monsa..er I mean HLVd virus!

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420 Garden

Well-Known Member
I can see the shifty folk out there saying " ima go to wallmart, gets me a microwscop. You get to workin on the web site thing. Come the weekend send me your leaf and i will check it for you. $12.95 ". venmo Paypal and cash.
 

Drop That Sound

Well-Known Member
I'm gonna build a clean room grade tissue culturing lab into an old RV\box truck, and drive around helping farms preserve all their genetics for cheap, and totally not make millions doing on site instant culturing. Work for donations and whatnot, but mostly for free, to help stop the evil corps from forcing their proprietary PGRs and modified genetics onto everyone to stop the epidemic.. Like a super hero. I'll even offer cleaning\IPM services, and fog out mom rooms, etc on the side. Deeefinitely not gonna get rich quick ;)
 

conor c

Well-Known Member
It’s already reported throughout the industry that a bleach solution of 10-20% kills the virus. So mother rooms need to stop being lazy and clean their shit appropriately.
Yeah man i had heard that bleach is more effective vs alcohol but still never hurts to double check
 

hotrodharley

Well-Known Member
Yeah man i had heard that bleach is more effective vs alcohol but still never hurts to double check
It’s not a virus eh? It’s viroid and the difference is vast.

“Although there is no chemical treatment available for controlling HLVd disease, methods such as meristem tip culture [32], thermotherapy [33] and cold treatment [34] have been found to reduce viroid titer significantly.Mar 4, 2023”

 

hotrodharley

Well-Known Member
You’re wasting supplies and efforts trying to kill this because it’s not alive in the sense of dying. It’s a single RNA strand. The bleach cleaning of equipment is advisable for preventing or controlling other pathogens but you’re cracking rat turds trying to mitigate this. WHY IT IS SO DANGEROUS TO THE INDUSTRY! Think these places collectively haven’t tried just about anything to get on top of it?
 

Boatguy

Well-Known Member
You’re wasting supplies and efforts trying to kill this because it’s not alive in the sense of dying. It’s a single RNA strand. The bleach cleaning of equipment is advisable for preventing or controlling other pathogens but you’re cracking rat turds trying to mitigate this. WHY IT IS SO DANGEROUS TO THE INDUSTRY! Think these places collectively haven’t tried just about anything to get on top of it?
"The removal of viroid-infected plants, and the sanitation of equipment, tools and of the vicinity of the viroid-infected plants are critical to checking the spread of the viroid in both fields and in indoor growth facilities. Heating blades at 160 °C for 10 min was found to be effective, while doing so at 140 °C was not. These measures have seen the virtual elimination of HSVd from the Kirin Brewery yards in Japan [10]. Among the different heat and chemical treatments tested for tool sanitation against viroids, the most reliable and widely used one is an aqueous solution of at least 5% household bleach (sodium hypochlorite, minimum 1% available chlorine)"

Directly quoted from the paper you linked. You should read it
 

hotrodharley

Well-Known Member
"The removal of viroid-infected plants, and the sanitation of equipment, tools and of the vicinity of the viroid-infected plants are critical to checking the spread of the viroid in both fields and in indoor growth facilities. Heating blades at 160 °C for 10 min was found to be effective, while doing so at 140 °C was not. These measures have seen the virtual elimination of HSVd from the Kirin Brewery yards in Japan [10]. Among the different heat and chemical treatments tested for tool sanitation against viroids, the most reliable and widely used one is an aqueous solution of at least 5% household bleach (sodium hypochlorite, minimum 1% available chlorine)"

Directly quoted from the paper you linked. You should read it

  • Sterilization: Bleach and hypochlorous acid also seem to work. For large grows, expensive water electrolysis machinery can be used to spray plants twice a week. Small operators can buy relatively inexpensive jugs of these disinfection mixes and literally spray, soak, agitate and brush plants individually to prevent spread.
Still, Jones cautions, none of these methods are failproof. Plants can test clean, then wind up with HLVd down the road. “It can be latent in the plant, then you can stress the plant out and it comes back again,” Jones says. “Just because you’re free of the virus doesn’t mean you’re free of the viral load.””

Start spraying or soaking your plants in bleach or hydrochlorous acid then. Even then it’s very possible to re-emerge down the road.

Again I seriously doubt the industry would be so taken over if simple bleach was effective.

 

jimihendrix1

Well-Known Member
Theres also another unnamed/unidentified plague to me, and many others thats affecting weed.

For one thing. We have been growing weed since 1978. And in reality I grew my 1st plant in 1972. But got serious interest when they started using HID for indoor gardening, in 1977-78.

But we, especially my buddy, he was the most outdoor cultivator, and me more indoors.

But in about the past 10 years, we, and others from Hawaii-South Africa have noted a purple/black type of disease, for any other realy name for whatever it is.

And NO. Its not genetic. Its not because of cold weather, to much water on leaves, phosphorus deficiency, PH.

My guess after dony some reserch is that it is a Phytoplasma, transmitted by bugs, mainly Leafhoppers. While leafhoppers have always been around, Ive never seen so many leafhoppers as that Ive seen the last 10 years.

The first sign usually starts right before flowering, though it can start earlier, but the general observation by my buddy is it comes on right before they switch to flowering.
It starts out a a slight purple tinge, in the growing tips, and spreads to the leaves, and stems/stalk. It starts out as light purple, and at its worst, turns into a deep purple black, which pften spreads to the whole plant, killing it. Though sometimes they grow out of it, but usually compromised, though some are ok..
This had been noted over the past several years on this forum, and a few other forums. THCFarmer probably having the most onfo and photos and experiences with the disease.

Like I said, I theorize its a bacterial/viral infection/phytoplasma, carried mainly by leafhoppers, but can also be transmitted by other chewing bugs.

Over the last 10 years my buddy has lost at least 2000 plants. Maybe more. It never happens inside using the same genetic material. It also happens in plots 20 miles apart. And as has been noted, from Hawaii, to South Africa.

Heres some photos.

1685897613602.png1685897672503.png1685897721318.png1685897757632.png
 

secretmicrogrow420

Well-Known Member
Theres also another unnamed/unidentified plague to me, and many others thats affecting weed.

For one thing. We have been growing weed since 1978. And in reality I grew my 1st plant in 1972. But got serious interest when they started using HID for indoor gardening, in 1977-78.

But we, especially my buddy, he was the most outdoor cultivator, and me more indoors.

But in about the past 10 years, we, and others from Hawaii-South Africa have noted a purple/black type of disease, for any other realy name for whatever it is.

And NO. Its not genetic. Its not because of cold weather, to much water on leaves, phosphorus deficiency, PH.

My guess after dony some reserch is that it is a Phytoplasma, transmitted by bugs, mainly Leafhoppers. While leafhoppers have always been around, Ive never seen so many leafhoppers as that Ive seen the last 10 years.

The first sign usually starts right before flowering, though it can start earlier, but the general observation by my buddy is it comes on right before they switch to flowering.
It starts out a a slight purple tinge, in the growing tips, and spreads to the leaves, and stems/stalk. It starts out as light purple, and at its worst, turns into a deep purple black, which pften spreads to the whole plant, killing it. Though sometimes they grow out of it, but usually compromised, though some are ok..
This had been noted over the past several years on this forum, and a few other forums. THCFarmer probably having the most onfo and photos and experiences with the disease.

Like I said, I theorize its a bacterial/viral infection/phytoplasma, carried mainly by leafhoppers, but can also be transmitted by other chewing bugs.

Over the last 10 years my buddy has lost at least 2000 plants. Maybe more. It never happens inside using the same genetic material. It also happens in plots 20 miles apart. And as has been noted, from Hawaii, to South Africa.

Heres some photos.

View attachment 5296745View attachment 5296747View attachment 5296748View attachment 5296750
holy smokes i was about too come on here and say MY CLONE HAS HLVD! because its not frosty like my other plants and the main top nug looks sick but after seeing your photos i realize i have nothing wrong with my plants you sir have a real problem on your hands!
 
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