Plant Tissue Culture

researchkitty

researchkitty

Well-Known Member
canndo said:
The think about mother plants is, unless you are outside, the things cost money to maitain. I had two main goals in trying to do this. The first was to eliminate my grove of mother plants that I saved just in case I wanted that strain again. The other was to collect more strains. How much do you figure your mother plants cost a month?
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My mothers cost less than a few dollars a month to maintain. I have 6 of them. Its just simple nutrients and water, what upkeep do you refer to? :) Clipping clones? At least they can flower, PTC dont until someone shows some proof?
 
canndo

canndo

Well-Known Member
Researchkitty. You are correct and I have not gotten to that point myself. I tend to take things rather slowly in order that I can truely be competent in each phase of anything I do. I have not even gotten a plant to grow in soil (my ultimate goal) from Tissue and so everything is theoretical. What I will say is that very few of my plants, in 9 months have died and they multiply quite rapidly. This, however I promise you. If there is any ongoing interaction on this site and this thread I will continue to post my progress with pictures. You are not the only one who claims that nothing has yet been demonstrated and honestly I don't actually know I can do it.

Let's see.

I'll post pics of the same set of culture evey few days. I will attempt to root them when they are large enough and if that is successful I will go on and transplant them to soil. I don't have the facilities currently to flower in my house. Is a 1 foot plant proof enough or is it necessary to get all the way to harvest?

DSCF0885.jpgDSCF0884.jpgDSCF0887.jpgDSCF0886.jpg

(the first two pics are from shoots from a previous culture, the other two are being established from a plant)
 
pointswest

pointswest

Active Member
researchkitty said:
Till someone shows me a grow started from plant tissue followed through in harvest, I declare everyone to be full of shit. Photos and proof =) These PTC threads are a dime a dozen on every pot site, and nobody has a successful grow to show from it. Nobody with real pictures showing a plant started in tissue and follow through to an actual harvest. All we ever see is a tiny picture and then the plants die and the poster never comes back.
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There are four stages to tissue culture before the plant is ready for transplanting into the final growing container. All these home trials are in the first stage and many perils await the growing process in the next three stages, especially in unsterilized growing areas. Some of these threads show vials of agar filled with mold, but the grower is claiming success, but in reality it is failure.

Can this be done? Yes, tissue culture is done on an everyday basis in the green industry, but it is in tissue culture labs that cost millions of dollars. The average home grower has very little chance of success with tissue culture methods. Cloning MJ is very easy to do with many different ways of propagation and people would be more successful if they would master one of these techniques. In only a few weeks you can root a cutting and have it ready to transplant, tissue culture would take many months to reach the same size. Stop wasting your time on extremly advanced propagation techniques and learn to clone.
 
canndo

canndo

Well-Known Member
No mold in my jars Pointswest. Four stages are establishment, multiplication, rooting and hardening off. I've accomplished establishment and multiplication. The establishment phase takes about 3 weeks and I've taken shoots from these and transplanted them without much trouble. I have pictures of all of it. It does work and so far it isn't as impossible as you make it out to be.

Does it take longer than taking a traditional cutting and rooting it? At first, yes but it doesn't take long at all before you have more plantlets than you know what to do with. I have what amounts to 50 moms now on one shelf using 20 watts of light, no watering, no feeding.

Will they root? we will see shortly. Will they flower? well, theoreticaly yes but we will see that as well. I see the only tough part being hardening off - it seems that managing humidity and light are the key issues and we all do that in every closed room grow.


DSCF0942.jpg This little plant is 2nd generation. There is still some apical dominance but a cytokinin tweek gave me better results as you can see in the next photo.

DSCF0944.jpg You can't see the shoots from here and unfortunately it is hard to photograph these things through all of the condensation but there are three shoots. I expect to be able to take these in about a week.

This one below is 3rd generation, under a week since they were transfered, these all came from a single in vitro plant.
DSCF0948.jpg

This is the establishment phase, this plantlet was cut and transplanted 6 days ago, there were no leaves when it was inserted into the media.

DSCF0950.jpg

I see where you are coming from but I must tell you that this is very possible - I have no extensive million dollar lab and my degree is not in botany or biology.
 

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pointswest

pointswest

Active Member
You have done well up to this stage. I am not talking about mold in your jars, but others who are trying this technique. Tissue culture works well for cloning hundreds of the same plant, but most growers only need a few plants which could easily be cloned in a few weeks. You have been very sterile in your procedures so far, I hope your success can continue in the later stages and grow to flowering. I am only trying to point out this is a technique for experimenting, but not too practical for the home grower unless you are selling clones in bulk to dispensaries. Clones propagated by conventional rooting methods are hardier and grow with more vigor.
 
canndo

canndo

Well-Known Member
Pointswest, I am led to believe that PTC starts are just as vigorous if not more so than more conventional cuttings. In my state there is a considerable market for branded clones both for growers in bulk and for the random consumer of only a few. Of course there are some who would like to keep viable libraries of assorted strains as well - we will see how that works out but I have my doubts. As I said, I don't see much of a problem rooting. I don't know if the roots will be effective however. If not, then I will go directly from shoots in vitro to soil. I believe I have come up with a method of hardening but we will see on that as well. You are welcome to follow my progress or failure as the case may be.
 
S

SWGreenThumb

Member
Did this thread die again? I just came across this thread on google. A friend recently brought by a TC kit he bought a few weeks ago.

I'm curious to try it out, but I am kind of wondering if anyone has had any sucess to report?
 
researchkitty

researchkitty

Well-Known Member
SWGreenThumb said:
Did this thread die again? I just came across this thread on google. A friend recently brought by a TC kit he bought a few weeks ago.

I'm curious to try it out, but I am kind of wondering if anyone has had any sucess to report?
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I've kept tabs on it and actively watch for it as a new subject, so far nothing still.......... How about you bite the bullet and buy a kit and photo document its progress? :) We'd all give ya big high fives!
 
canndo

canndo

Well-Known Member
I have been showing all sorts of "success" on my journal of the subject. The kit is not enough, TDZ is the key. I am halted due to heat concerns and am outfitting a refrigerator as a new incubator but there are plenty of pictures of my progress.
 
Hydrotech364

Hydrotech364

Well-Known Member
Living Tissue Only?Would be nice to be able to reanimate whenever you get a batch that you would like to grow yourself.Tell Me More....Plzzzz
 
researchkitty

researchkitty

Well-Known Member
Many more months have passed, many more have tried, and never are able to post a plant that is able to be flowered for harvesting............. They always die as kittens invitro! <G>
 
thump easy

thump easy

Well-Known Member
image.jpg image.jpg I'm gona get a hood flow and a real hepa space but hardening the plantlets is hard this shit takes to long
 
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