bought spore syrenge, sterilized substrate/jars, and perlite for fruiting chamber. Will I need anything else like vermaculite and bandaids? Ive been trying to find info of a start to finish guide but all Im finding is scattered info.
-thanks for your time
You will need
Rubber gloves
Can of Lysol
rubbing alcohol
Face mask if you can find one
Cotton "rounds" (pads) or Cotton balls
a cigarette lighter. (no child safety if you can)
medical tape
permanent marker
A storage bin (to keep jars dark, and from drying out during incubation)
can of red bull (lol)
first set up a table in a small room (ive even used a large cutting board on a bathroom sink before)
close all air vents, turn off all fans
wipe down the surface with alcohol.
spray the shit out of the room with lysol, the entire ceiling, and let it float down.
close the door and let the room sit for 30 min or so.
while that is sitting, go take a good hot shower wash your hair real good.
Brush your teeth, and use mouth wash.
put on the gloves and face mask before re-entering the small room.
set aside a cotton round/ball wet with alcohol. Not too much, you dont want an alcohol pool. or you could accidentally catch your table on fire.
flame the needle of the syringe until its red hot.
then wipe with the alcohol cotton pad to cool.
then inject the cakes with the syringe. you want the needle to be pressed against the glass.
stare at the tip of the needle, and then apply pressure to the plunger.
you want just enough so some liquid comes out, and gets sucked up by the cake, or slightly dribbles down the glass.
if you inject 4 holes per cake, they will colonize twice as fast.
if you inject 2 holes per cake, you can innoculate twice as many cakes.
Do you want time? or yield?
place medical tape over needle holes. and mark with strain, and date.
Place in storage box, and if i were you, i wouldnt check on them for 10 days.
Why? because it is highly unlikely that they will finish in less then 10 days.
and it does no good to look at them.
so in 10 days, you might have really good growth. or you might have contaminates. or you might just be starting to show growth.
but regardless there is nothing you can do to intervene.
once the jars are 100% covered in white, mark them with the date that they finished. and then wait another 7 to 10 days.
there is usually a jar or two that are quicker then the rest. you could wait till the majority catches up. and then wait 7 to 10 days to birth them together.
there is also usually a couple jars that are slow. dont worry about those.
the reason for waiting, is just because the surface gets covered, dosnt mean that the jar is 100% colonized.
It will still take another week or so for the fungi to colonize the core.
if you birth early, the cakes will just sit in the fruiting chamber, and do nothing for 7 to 10 days anyway, while it colonizes the core.
and during that time you are going to be freaking out over why its just not fruiting.
what happens, is once the cake is 100% colonized, the fungi will realize that it is out of food. and freak out.
it will then switch from colonize mode, to fruit mode. in order to save itself. right before this happens you can sometimes even see little fingers poke out of the cake, reaching out, looking for food.
once your cakes are 100% and the 7 to 10 days has passed, pop the cakes out of the jars, and rinse them in the sink with gloved hands.
and then soak under water for 24 hours.
then roll them in dry verm. and place in the fruiting chamber.
the most important part of the fruiting chamber, is misting the walls. trying to get as many water droplets to stick as possible. make it a game.
then monitor the water drops, to determine the humidity level. if most of the droplets are there, your good.
if they start to disapear, then the humidity is dropping, and just like the humidity is leaving the walls, the humidity is also leaving your cakes.
make sure to mist your cakes directly, but dont blast them point blank. you want them moist.
i hate the perlite fruiting chamber. and the shotgun fruiting chamber.
my favorite fruiting chamber, is make two quarter size holes, on two ends of the chamber, and stuff with polyfill pillow stuffing.
make the holes about a quarter inch, to an inch off the floor of the chamber. just enough so that pooled water dosnt leak out.
to this you can add a air pump with a cup of water and air stone.
this will create positive pressure, and push co2 out of the polyfil holes. and bring in fresh o2.