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Dude, yes, they are all pretty much the same, however, they do not all fruit in perfect sync. Had you just used the expression that put up all those primordial you might have had a HUGE flush. Multiple expressions will fruit in succession but the earliest will hog all the nutrients. Your best...

That a multi culture? Those primordia all over the sides there. They should be coming up.

Check my recounting from years ago on this site. I managed it, with pictures no less. https://rollitup.org/t/tissue-culture-micro-propagation-grow-from-scratch.409965/page-6#post-5548948

This is an old series of pictures I posted nearly 10 years ago. This is simply to show how well casing layers can work. The result of orchestrated fruiting on casing.

Ah! Everyone has a system and most of them work. Why? Because cubs and oysters fruit in spite of the grower. This is not to diminish your efforts here, I am just trying to have a conversation which may benefit others reading along. I have always approached my endeavors here as being...

If we simply lay more substrate on colonized substrate all we get are delays and possible contamination. And no signal. A true casing layer (the term is badly misused, I have even seen the term "casings" as though that means anything at all. They may even be perverting "castings" which is...

Bestcoca Or cocaseeds Dot com.

No. Casing is different than substrate. Casing is a non nutritive, high water absorbent topping that signals the mycelium as a single organism that it is out of food while it imparts water to that mycelial mass. Coir is nutrient rich.

What are you using as casing again?

Dude. ASK YOUR DOCTOR. Not some unknown pot heads on the internet.the

On "dunking". I have weighed fully colonized grain before and after soaking for several hours. I never saw much weight change. I can't dispute that *something * happens afterward but I don't think it has much to do with water absorption. Mycelium tends to repel moisture. Sort of.

Not sure your breakthrough is evidence of excess co2. Nutrient in casing? Casing too thin? Shift to fruit too late? But yeah, you are close to overlay. BTW, thanks for the over all tour.

Like the poor, assholes will always be with us. This ONE thread in all the thousands was erected to be anti pf. (Maybe do a syringe of half pints first) doesn't look like an advance to "bulk". Yet again. Pf tek was designed to rip you off. It was designed to sell syringes and nothing...

Grafting is not just for slow growing plants. All my tomatoes are heirlooms grafted to strong root stock. I don't have any problems with soil born pathogens or nematodes. Micro grafting can have sucess rates of 90 percent. Mine are commonly in the 70's. I can't see where this technique...

Why would you post this on a thread that is specifically anti pf?

It's not "too sciency" at all. Take a stem from as non woody a place as you can. Make a cut in another non woody place Align the cambrium as much as possible. Keep the cutting from drying out as it heals. Two weeks should be fine. And that's it.

Prions are not spores. In fact, they are not organisms. Nor, I believe, will you have problems in mycology with such misfolded proteins

Shake the jar, that's what whole grain is for

Sterilization is a relatively process. You could sterilize In five pounds of pressure if you want to keep it there for long enough. What is the load of the substrate? Is it heavily contaminated? What IS the substrate? Clear water water will become sterile sooner than heavily packed flour...

Put it back together, tamp it down and give it a few days at colonizing temp and humidity. Then recase. It will be fine.