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Do it. There were no ready made bags when this was written.

Consider casing.

A month? Shouldn't be any more than 10 days. I appreciate the work you put in here...but. No need for gas exchange so long as you use half jar. Be sure you carve out the out side of each plate before you "pizza" it. The region next to the edge of your plate is the most likely to be...

W Wrong. But the odds of your actually getting the results of a single spore are pretty slim unless that is what you are intending. I have to dilute solutions to hundreds to one in order to maybe have that happen. And they DO have sexes, in other words, two random spores may not deliver...

While ryzomorphic mycelium may or may not signify robust features, one can be relatively assured that the ends of those strands in a sector on a plate is a single culture. The cultures tend to organize themselves and the ropey parts are likely pure where the more tomatose samples may be mixed.

It's not. It colonizes where spores land.

Ok If you are indeed growing single spores, they will never fruit. Isolating from single spores won't ever fruit either. It is also unlikely that they will get rizomorphic.

It's fine It will likely repair itself anyway.

Please be careful with those old pressure cookers. Expansion and contraction cycles put tiny cracks in the metal And be sure your fail safes are new. I had a failure once. My wife was seconds from being scalded as a cooker blew as she was getting in to check it. It steamed all the paint...

I met kesey several times. He didn't much like me.

Take a bit of the inside of a fruit you like. Place it on agar. Wait You now have a monoculture. Or you can grab a strand of mycelium at the furthest portion from the center. Place that in a new dish Odds are you have a monoculture. If not do the same thing on your new dish.

Probably, but I have surprised at how I could spray insecticide in another room and still get rosecomb

Either genetics or exposure to hydrocarbons.

What are we looking at? Looks like a pure spot of the sort of mycelium I presume you want. Am I missing something?

Easy way to isolate a single culture...if you hadn't already done so. Yes ganjuh it is, with a few fruit on top.

As many cc as you can afford but not so many as floods your substrate.

Yes. But it takes a bit. They say lc can be caramelized and that inhibits growth. I'm never sure. Nor do I know if a "mere" 212 is enough to do that.

Ok, pe was not genetically engineered in the DNA sense. Nor was it any more "grown in the lab" as any other land race. Isolation of desirable expressions by agar sectoring is a good thing but it isn't normally meant to "clean" the mycelium. One does that when attempting to separate your...

I'm very sorry, there seems to be no such thing as a safe, AND continously effective anxiolytic. I think it's some kind of cosmic law.

Doesn't look quite right. But I always have trouble identifying the damn things.