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ndangerspecimen101

ndangerspecimen101

Well-Known Member
Sorry for the intermittance in this thread :lol:

But I'm about to inoculate the rest of the seven jars with a half cc.

I have a theory why the other seven might of not flourished. I think I might of over heated the needle when sterilizing, because my needle was shaking when I was doing this... could the needle become so hot that the actually suspension inside become hot.... causing the spores to die?
 
estesj

estesj

Well-Known Member
ndangerspecimen101 said:
Sorry for the intermittance in this thread :lol:

But I'm about to inoculate the rest of the seven jars with a half cc.

I have a theory why the other seven might of not flourished. I think I might of over heated the needle when sterilizing, because my needle was shaking when I was doing this... could the needle become so hot that the actually suspension inside become hot.... causing the spores to die?
Click to expand...
My needle was shaking and spitten out water it was so hot and I had no probs.
 
ndangerspecimen101

ndangerspecimen101

Well-Known Member
estesj said:
Just dident shake good enough maby so you may have injected strait water I guess.
Click to expand...
Odd enough, I did do a good tap and shake... I'm familiar with giving intramuscular and subcutaneous shots, so I definitely know how to work a syringe. All I do hope, is that this injection puts those jars into deep fruiting ;)
 
ANC

ANC

Well-Known Member
basicaly yes, I think your spores were clumped in a few balls... if you could see em especialy... My spore injections just look like water...

but it is important to make sure to try and get the solution to run down the glass around the sides of your jar, as it is hard to see colonisation starting from the inside out.
 
ndangerspecimen101

ndangerspecimen101

Well-Known Member
ANC said:
basicaly yes, I think your spores were clumped in a few balls... if you could see em especialy... My spore injections just look like water...

but it is important to make sure to try and get the solution to run down the glass around the sides of your jar, as it is hard to see colonisation starting from the inside out.
Click to expand...
My spore syringe was clumped with big spores, very noticeable! But some of the jars I inoculated I didn't run the needle by the sides... I injected towards the middle of the substrate. They could be growing inside, but will they ever make a clean visible break?

Anywho, will be inoculating in a few hours from now!
 
ANC

ANC

Well-Known Member
yea see, there are no big cube spores, they are microscopic, anything you saw was clumps of thousands of spores.

With grains yes, with pf tek maybe... think of the mycelial network as a transport system, when it works from the outside it has access to gass exchnage and can feed mycelium diving deeper into the substrate.
Starting in the middle of a wet fudge pf cake might obstruct this.
 
ndangerspecimen101

ndangerspecimen101

Well-Known Member
What do you recommend ANC...

If my spore syringes have large clumps... what is the best way to disperse them evenly in the suspension before inoculating?
 
ndangerspecimen101

ndangerspecimen101

Well-Known Member
Okay, I did the the little top on the sole of a shoe to disperse the mycelium some... however, some of the dry vermiculite on top fell down over some of the mycelium. Would this pose a contamination problem or stunt of the growth of the mycelium in any way?
 
ANC

ANC

Well-Known Member
Don't stress little brother, just watch nature do its thing.... and yeah clumped syringes are bad news, the best way to make syringes is to have a little soap in the water, just to break the surface tension.
 
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