Cloning Plants By Tissue Culture

alphawolf.hack

alphawolf.hack

New Member
you are right. im about to buy a kit i have done the math the costs to build your own are about the same as the kit kinda like aeroponics. but man its so off-putting knowing i cant get some of the chemicals/genes more easily.(yeah i stay up doing math and shit all night i spend most of my time designing systems i will never use or destroying old electronics for components)
 
PetFlora

PetFlora

Well-Known Member
pharmacoping said:
Get back to your "computer monitor clone warmer" or your xbox,or whatever you kids do while dreaming of producing marijuana for the masses.
are you culturing? please share some experiences, like wacky flowering times, or homemade additives, or something. nobody has time to cater to your negativity in this forum, until they get out of school, maybe at 3pm. everyone has read your posts thusfar, and must say, not so impressed by your information. . If you're so sure about these foundations of yours, why are you here taunting peolple with real life experiences.
If you are genuinely interested in my bio lumi mj plant, PLACE A BID NOW, public or private.
I will not give you these specific answers, as I might be here just to sell something, remember?

fyi, everyday, when each of us clones,or cultures, we are genetically modifying plant material, albeit near impossible on an Xbox warming plate, or a desk light for illumination.

PICS or GTFO wolfzen, really. we dont read words well, so if you feel the need to type more, just dont! and for the record, I Do think your mom is hot !
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OMG LMFAO! But it points out why I do journals, where I can delete idiots, but then I would not have seen your awesome reply

 
pharmacoping

pharmacoping

Active Member
PetFlora said:
OMG LMFAO! But it points out why I do journals, where I can delete idiots, but then I would not have seen your awesome reply
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wish I would have started a journal instead, but am a forum newby. wonder if it can be moved to a journal??
thanks for the yell

peace
 
alphawolf.hack

alphawolf.hack

New Member
okay im ordering the classroom kit with dvd for African violets (im gonna start there stupid to jump in with out an a good example) then im gonna fabricate my own media from what i learn. so in this process of making you plants glow it a cultured virus correct? could it where off as a plant grows? or has the virus literally changed the genetics of the plant? and in the second scenario how would one go about designing a virus to do this, or is there a virus that does this already?
 
WolfZen

WolfZen

Member
Making a plant glow requires genetic engineering methods, knowledge and equipment. Tissue culturing is not genetic engineering. Why do you keep implying otherwise?
 
pharmacoping

pharmacoping

Active Member
WolfZen said:
Making a plant glow requires genetic engineering methods, knowledge and equipment. Tissue culturing is not genetic engineering. Why do you keep implying otherwise?
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ok, to settle this for you;
humans have been tampering with genetics for centuries. selective harvesting,hand pollinating or cross pollinating, or inducing mutants, with mutated, genes, that may have been engineered by a plethora of bacteria/virus at many different stages in growth, engineered by combining its own dna, with the hosts' dna, creating recombinant dna. this is done with great success in my lab now, and many other home labs. yes, more knowledge than you are willing to accept, or posess, is necessary to perform these small miracles.

So, you see, just because we may not have created(engineered) genetic material, does not mean that we are not intentionally culturing it, harnessing it, selecting it, breeding it, and combining it with other dna, becoming the final engineer. Tissue culture is a tool for genetic engineering. genetic engineering takes place in tissue culture. genes may be altered during your sloppy clone cutting/warming practices.

I know this is rhetoric for you, since you've already read the book I've suggested, perhaps a reread is in order. This information is near the beginning, before the words get bigger.
 
alphawolf.hack

alphawolf.hack

New Member
okay got ya. about the meadia im not sure i understand. the meadia is agar with nutes and hormones added right? so i under stand how to get NAA and auxins but i have yet to see a conventional cytokin hormone (i have heard of coconuts milk but thats all i have found so far dont plan on using it mind you.) ii i understand what your saying is i will use agar as the media and my mixture of hormones and nutes will be what im fabricating.
 
alphawolf.hack

alphawolf.hack

New Member
all right well most of my preliminary ?'s have been answered im on-board and will get back with you guys when i get some stuff up and running. super helpful too. my bad again for kinda raggin on you. im just gonna sit back a pay attention to the info from here on out or at least till im in the middle of my process and go oh fuck.
 
TaoWolf

TaoWolf

Active Member
pharmacoping said:
ok, to settle this for you;
humans have been tampering with genetics for centuries. selective harvesting,hand pollinating or cross pollinating, or inducing mutants, with mutated, genes, that may have been engineered by a plethora of bacteria/virus at many different stages in growth, engineered by combining its own dna, with the hosts' dna, creating recombinant dna. this is done with great success in my lab now, and many other home labs. yes, more knowledge than you are willing to accept, or posess, is necessary to perform these small miracles.

So, you see, just because we may not have created(engineered) genetic material, does not mean that we are not intentionally culturing it, harnessing it, selecting it, breeding it, and combining it with other dna, becoming the final engineer. Tissue culture is a tool for genetic engineering. genetic engineering takes place in tissue culture. genes may be altered during your sloppy clone cutting/warming practices.

I know this is rhetoric for you, since you've already read the book I've suggested, perhaps a reread is in order. This information is near the beginning, before the words get bigger.
Click to expand...
You haven't had any success that you've shown nor do you have enough knowledge to discuss your claims without getting upset by direct questions. Again, tissue culturing doesn't alter DNA or manipulate DNA no matter how obtuse you want to get about it. Tissue culturing is simply culturing tissue in a media.

Trying to compare tissue culturing to genetic manipulation/engineering is like equating knowing how to fill a car's gas tank to being an automotive engineer. What's the point in misleading people like that unless you are selling gasoline?
 
alphawolf.hack

alphawolf.hack

New Member
why didnt you consult a dictionary b4 you came to this thread



Definition of GENETIC ENGINEERING merrium-webster

: the group of applied techniques of genetics and biotechnology used to cut up and join together genetic material and especially DNA from one or more species of organism and to introduce the result into an organism in order to change one or more of its characteristics
See genetic engineering defined for English-language learners »
See genetic engineering defined for kids »

so according to this definition grafting and tissue culture are genetic engineering.
 
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