Cloning Plants By Tissue Culture

alphawolf.hack

New Member
you are taking specific DNA and culturing it thus genetic engineering. there is no way this cutting would survive with your genetic intervention of adding hormones to alter DNA of cell to allow them propagate and create roots and shoots.
 

WolfZen

Member
This is a forum, wild claims will probably be questioned, whether for fun or not. That's the way forums are.

You could always sell your TC kits legitimately and pay for advertising if you don't want interaction with forum members?
 

WolfZen

Member
you are taking specific DNA and culturing it thus genetic engineering. there is no way this cutting would survive with your genetic intervention of adding hormones to alter DNA of cell to allow them propagate and create roots and shoots.
Would have been a good point except that you aren't altering or manipulating basic cellular DNA by using hormones. Even by layman's terms using hormones is not 'genetic engineering'.
 

WolfZen

Member
That is how much u know,,think this is a kit thing....uuuuuuuuuuubbbbbbbbmmmmmmmbtbtbtbtbtbtbtb..
Just growin plants son!!!!
pharmacoping said:
I do this very well, and assemble kits for sale too /prepared solutions/pre filled sterile containers/proper nutrients, and even genetic specimens, for observation purposes. Some samples include Thyme, Rosemary, Tahoe OG Kush,PlushBerry,Kandy Kush, and hundreds of other non plant dna samples. The study of these samples will can prepare you for the art of tissue cultivation/cloning. From a single spec thousands of master programmed clones can be taken monthly ! Imagine no more mother plants or clones using your plant spaces? How important is that to you? These are stored/divided as masses of tissue or unusable roots. Only in the last few days do they become a "plant". You can easily have roses with thc, or mj without leaves, or even glow in the dark, really, I've seen it ! It's not the future, it's now ! Patents are already being granted for different mj types grown this way. suicide genes,inability to clone/seed..are some of the programing in these super corpopharma plants. Instructions are available online, but the right mixes are almost impossible to find and invaluable to growing your own rare herbs successfully, and storing their dna forever. With these supplies you'll learn how to master this fast because the work is done !! Plan on spending a couple hundred bucks or less, depending on the equipment you have at home...jars/mixer/pressure cooker,etc.

I'll answer some q's here, but private message me for instructions for above.


Want kind of rice car do you have by the way?
 

PetFlora

Well-Known Member
FYI: A few decades back I worked a company named AccuDerm. They were 'famous' within the Dermatology for their version of agar colloid. I believe they are located in Fort Lauderdale, but not sure you can buy without being a dr.
 

pharmacoping

Active Member
You haven't had any success that you've shown nor do you have enough knowledge to discuss your claims without getting upset by direct questions. Again, tissue culturing doesn't alter DNA or manipulate DNA no matter how obtuse you want to get about it. Tissue culturing is simply culturing tissue in a media.

Trying to compare tissue culturing to genetic manipulation/engineering is like equating knowing how to fill a car's gas tank to being an automotive engineer. What's the point in misleading people like that unless you are selling gasoline?

ASSHOLES LISTEN UP !

John Kleyn-chapter one, tissue culture overview; phd cornell university, plants from test tubes, again.. this is why q's are not answered. first you must understand the fundamentals of culture, and it's practices, then a grasp of it's reality may share a glimpse

"Tissue culture is a clean and rapid way to grow material for identifying and -manipulating- genes, or to -tranfer individual characteristics from one plant to another"

you are wrong sir.


radiation causes genetic manipulation, uv light causes it to, electricity causes it also, so does a magnetic field....all of which may or may not be happening within our home labs.
if a plant is infected, it's genetics are changed, even if slightly, but if you purposely infect a plant, changing is dna, or mixing other foreign dna, you have become the genetic engineer of that creation, and furthermore, you would be hard pressed to locate a botanical genetic engineer that does not perform most of his lab duties in tissue culture.

dammit all, get over it !
 

Bonkleesha

Active Member
HEY, DUDE! i got my test tube from the microprop lab (the chuting one.. we transferred our new chutes into the rooting media). anyways theres like 2 inches of media in it. if i am careful and sterile, and i take about half an inch out where my miniature rose was and use it for cannabis?
 

alphawolf.hack

New Member
whats up pharm. hey man okay so i just finished book 3 on this subject got a couple a ?'s for you... do you sterilize the media nute hormone ect.? or just the container? isn't all that stuff supposed to com prepacked and sterilized or is this just a safety measure? also once you reach the stage where plantlet is cultured in hormone free media how long is shelf life? as far as degrading genetics does this only apply to callus cultured cells? damn i learned alot.

bankleesha you werent around he said togo4it.
 

pharmacoping

Active Member
FYI: A few decades back I worked a company named AccuDerm. They were 'famous' within the Dermatology for their version of agar colloid. I believe they are located in Fort Lauderdale, but not sure you can buy without being a dr.
They have this bad ass seed encapsulation system, for our artificial seeds. when we make these seeds, they cannot be stored except in agar, and AccuDerm solved that with a wrapper ! I use colloidal agar also, and am damn tempted to culture human cells, but I'm scared. I've done the glo on the animals found on on raw shrimp and its pretty wild bacteria. played with the glo in fireflies for a long time in vitro. I used colloidal agar but got huge results with a beef brine. bioluminescence was occupied my thoughts since childhood. it was the very first reason I cultured over 30 yrs ago, never stopped.

thanks for sharing this, it shed some light on my materials..haha, literally. heres a shot for you, recognize the agar ??
 

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