Dark Side Of The Moon.....

anemones live beside volcanic vents miles below the ocean..pressure and heat cant kill everything,everytime..i allways have taken a little failure in the equation..if you want 5 jars,make 8..if they all are fine,choose the best 5..better than having less than you wanted...

It is a game of numbers, certain endospores can survive high, dry heat. Moist heat will kill a large percentage of them in a few minutes, a few more in more time, and so on. Further, just because your PC is up to pressure doesn't mean every thing is at 250. The denser the material, the tighter your PC is packed, the longer it takes to kill every endospore. when I do large bags of grain I go an hour and a half after normalization (no longer having to dick with the heat settings). You can also germamate the spores by soaking your Substrate for a day or so, making them more liable to be killed. Endospores are tough little suckers.

Oh i am seeing the light. dont run towards the light no oh its only my hps whew. joking. I am following you. if you dont have a sterile clean room you will get contamination no matter what so just make more and plan to loose a few.

canndo said:

It is a game of numbers, certain endospores can survive high, dry heat. Moist heat will kill a large percentage of them in a few minutes, a few more in more time, and so on. Further, just because your PC is up to pressure doesn't mean every thing is at 250. The denser the material, the tighter your PC is packed, the longer it takes to kill every endospore. when I do large bags of grain I go an hour and a half after normalization (no longer having to dick with the heat settings). You can also germamate the spores by soaking your Substrate for a day or so, making them more liable to be killed. Endospores are tough little suckers.

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and minimize your losses by having good sterile technique

DCobeen said:

I never trust when they say its sterile. i always run boiling water through mine when im gonna can. so that is best practice Dank. see germs and bacteria cant live above 180 degrees F. so if you boil water and submerge them in it them remove and sit upside down on paper towel then load and use it you will be allot better off.

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yeah, I have been using a pressure cooker. The new jars/cakes I made up using the WBS & PC I have yet to see any signs of contaminate period. All 4 jars/cakes are already showing signs of colonization (way before their pre sterile jars). So from now on I will be making my own. I started seeing growth out of both WBS cakes before the PC cakes. However now the PC cakes have took over and are spreading. 2 out of the six jars that I had received with the grow kit are looking contaminated. Thats another reason why I went ahead and made up the 4 extra jars. I have 2 of the pre sterile jars that's not showing any signs of being inoculated at all. So I will have to set those aside while the ones fully colonized are in the fruiting chamber. I received my reptile mister & air pump yesterday, so now all we are waiting on is the cakes to fully colonize.

The biggest pro lem wit substrate "concoctions" is that you can't shake them. Now all you can do is wait, if you were using a whole grain, you would be on to the next step already, and, your substrate would not be unevenly consumed.

On with the show. I just made up 3 new jars. 2 of the 3 I used BRF & V + H20 mix. The 3rd out of 3 I used the some new WBS. I just received in 3 new strains. More Golden teachers, Pesa, & Golf Coast. I will be inoculating those 3 cakes as soon as they cool from being sterilized. All the others have been colonizing slowly but surely. Looks like the popcorn & wild bird seed cakes are moving along faster then the previous ones inoculated before them. Mexican -> 24K -> 24 K -> Golden Teacher -> Mexican -> colonizing jars -> reptile fogger for terrarium when ready ->

again, thanks 4 following the grow..

sweet fogger wow. i love it.

sweet getter done dank, love this thread good job playa

YUP.. Thanks. I was thinking after reading what all Canado had wrote. That they need higher humidity, and its hard to get the humidity high having to take the lid off the box. So I was thinking to myself. lol If I where a Shroom, what and or where would I like to live. I was thinking about trying to create a "amazon" environment 4 them, went and purchased 2 big dark totes, got the fogger in, and also the air pump. Going to start working on the terrarium today.

DCobeen said:

sweet fogger wow. i love it.

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Thanks bro.. Sheww.. I'm trying. lol.

bigworm6969 said:

sweet getter done dank, love this thread good job playa

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~Dankster~420 said:

YUP.. Thanks. I was thinking after reading what all Canado had wrote. That they need higher humidity, and its hard to get the humidity high having to take the lid off the box. So I was thinking to myself. lol If I where a Shroom, what and or where would I like to live. I was thinking about trying to create a "amazon" environment 4 them, went and purchased 2 big dark totes, got the fogger in, and also the air pump. Going to start working on the terrarium today.

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Im telling ya man, you can save so much money by building a SGFC if your just doing cakes. Its also good for bulk trays. I am currently running 2 SGFCs and they are a constant 99% humidity . No foggers, air pumps, etc etc etc needed.

JJ05 said:

Im telling ya man, you can save so much money by building a SGFC if your just doing cakes. Its also good for bulk trays. I am currently running 2 SGFCs and they are a constant 99% humidity . No foggers, air pumps, etc etc etc needed.

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but you give up control of your fruiting space. And what if you want 95 percent? And how do you manage air exchange? His fogger is akin to the plenum I was talking about and is far superior to other humidity approaches.

canndo said:

but you give up control of your fruiting space. And what if you want 95 percent? And how do you manage air exchange? His fogger is akin to the plenum I was talking about and is far superior to other humidity approaches.

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I manage air control by fanning 4-5 times a day. I can let my humidity drop into the 90-95% region if I dont mist the perlite as frequently as I do. Heres a pic of a terrarium setup I seen and boy oh boy...its fucking SCARY!









I would be afraid to open that terrarium! Id literally throw the entire thing away lol! WTF is that stuff!?

cobweb mold. I read something (not primary lit.) saying that it is/ could be a parasitic mold that uses the intended culture as a host since the person said they (and I) have never seen pics of cobweb on grains and that have not been inoculated with anything else (the person tried to noc them up with tissue transfers).

Ok, I didn't even get past page 2 before the questions started forming in my melon ..... so I apologize if this has been covered already, but would mushrooms do well in a marijuana grow environment? With the CO2 that they put off it seems like a great companion to grow along side herb.

I'd like to try shrooms again. I only did them one time, and ate an 1/8'th by myself per the advice of my buddy ..... and holy shit was I messed up. I couldn't even move after a while. Trippy and amazing, but too overwhelming for me. I'm thinking if I were to try them again a more modest amount might give me an all together different experience (maybe?).

JJ05 said:

I manage air control by fanning 4-5 times a day. I can let my humidity drop into the 90-95% region if I dont mist the perlite as frequently as I do. Heres a pic of a terrarium setup I seen and boy oh boy...its fucking SCARY!

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I would be afraid to open that terrarium! Id literally throw the entire thing away lol! WTF is that stuff!?

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the point of a plenum is consistancy and control. The op can manage co2 levels to a precise degree and still have small, smooth airflow over the entire growing surface, he can also reduce levels of contamination by placing a filter on the plenum intake and keeping a slight positive pressure in the fruiting chamber. All this makes for optimal yield in the shortest time possible. He can even go away for several days at a time.

and, we agree. That grow should never again be opened if the grower has any intention of growing in his space or house again.

technical dan said:

cobweb mold. I read something (not primary lit.) saying that it is/ could be a parasitic mold that uses the intended culture as a host since the person said they (and I) have never seen pics of cobweb on grains and that have not been inoculated with anything else (the person tried to noc them up with tissue transfers).

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I have never seen cobweb grow on anything but overly wet casing, and then, as you suspect, on fruit.

st0wandgrow said:

Ok, I didn't even get past page 2 before the questions started forming in my melon ..... so I apologize if this has been covered already, but would mushrooms do well in a marijuana grow environment? With the CO2 that they put off it seems like a great companion to grow along side herb.

I'd like to try shrooms again. I only did them one time, and ate an 1/8'th by myself per the advice of my buddy ..... and holy shit was I messed up. I couldn't even move after a while. Trippy and amazing, but too overwhelming for me. I'm thinking if I were to try them again a more modest amount might give me an all together different experience (maybe?).

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the problem with growing in plant space is that in order to help induce fruiting, those high levels of co2 must be brought to low levels
. I have seen and experimented with wood loving mold in vented bags in order to raise co2 levels in the room. My problem was a steep curve. We got noticeable increases in co2 for a week or so, then a quick drop as the substrate was consumed. I think it would have worked better using a more coarse substrate.

canndo said:

the problem with growing in plant space is that in order to help induce fruiting, those high levels of co2 must be brought to low levels
. I have seen and experimented with wood loving mold in vented bags in order to raise co2 levels in the room. My problem was a steep curve. We got noticeable increases in co2 for a week or so, then a quick drop as the substrate was consumed. I think it would have worked better using a more coarse substrate.

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I see. Just wondering since those "Exhale" CO2 bags are just mushrooms ...... so I figured why not grow some special mushrooms if the concept worked.

Thanks for the response